AREB6 (ZEB1) (NM_001128128) Human Untagged Clone

CAT#: SC323255

ZEB1 (untagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZEB1
• Synonyms: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001128128 edited
  ATGAAAGTTACAAATTATAATACTGTGGTAGAAACAAATTCAGATTCAGATGATGAAGAC
  AAACTGCATATTGTGGAAGAAGAAAGTGTTACAGATGCAGCTGACTGTGAAGGTGTACCA
  GAGGATGACCTGCCAACAGACCAGACAGTGTTACCAGGGAGGAGCAGTGAAAGAGAAGGG
  AATGCTAAGAACTGCTGGGAGGATGACACAGGAAAGGAAGGGCAAGAAATCCTGGGGCCT
  GAAGCTCAGGCAGATGAAGCAGGATGTACAGTAAAAGATGATGAATGCGAGTCAGATGCA
  GAAAATGAGCAAAACCATGATCCTAATGTTGAAGAGTTTCTACAACAACAAGACACTGCT
  GTCATTTTTCCTGAGGCACCTGAAGAGGACCAGAGGCAGGGCACACCAGAAGCCAGTGGT
  CATGATGAAAATGGAACACCAGATGCATTTTCACAATTACTCACCTGTCCATATTGTGAT
  AGAGGCTATAAACGCTTTACCTCTCTGAAAGAACACATTAAATATCGTCATGAAAAGAAT
  GAAGATAACTTTAGTTGCTCCCTGTGCAGTTACACCTTTGCATACAGAACCCAACTTGAA
  CGTCACATGACATCACATAAATCAGGAAGAGATCAAAGACATGTGACGCAGTCTGGGTGT
  AATCGTAAATTCAAATGCACTGAGTGTGGAAAAGCTTTCAAATACAAACATCACCTAAAA
  GAGCACTTAAGAATTCACAGTGGAGAGAAGCCATATGAATGCCCAAACTGCAAGAAACGC
  TTTTCCCATTCTGGCTCCTATAGCTCACACATAAGCAGTAAGAAATGTATCAGCTTGATA
  CCTGTGAATGGGCGACCAAGAACAGGACTCAAGACATCTCAGTGTTCTTCACCGTCTCTT
  TCAGCATCACCAGGCAGTCCCACACGACCACAGATACGGCAAAAGATAGAGAATAAACCC
  CTTCAAGAACAACTTTCTGTTAACCAAATTAAAACTGAACCTGTGGATTATGAATTCAAA
  CCCATAGTGGTTGCTTCAGGAATCAACTGTTCAACCCCTTTACAAAATGGGGTTTTCACT
  GGTGGTGGCCCATTACAGGCAACCAGTTCTCCTCAGGGCATGGTGCAAGCTGTTGTTCTG
  CCAACAGTTGGTTTGGTGTCTCCCATAAGTATCAATTTAAGTGATATTCAGAATGTACTT
  AAAGTGGCGGTAGATGGTAATGTAATAAGGCAAGTGTTGGAGAATAATCAAGCCAATCTT
  GCATCCAAAGAACAAGAAACAATCAATGCTTCACCCATACAACAAGGTGGCCATTCTGTT
  ATTTCAGCCATCAGTCTTCCTTTGGTTGATCAAGATGGAACAACCAAAATTATCATCAAC
  TACAGTCTTGAGCAGCCTAGCCAACTTCAAGTTGTTCCTCAAAATTTAAAAAAAGAAAAT
  CCAGTCGCTACAAACAGTTGTAAAAGTGAAAAGTTACCAGAAGATCTTACTGTTAAGTCT
  GAGAAGGACAAAAGCTTTGAAGGGGGGGTGAATGATAGCACTTGTCTTCTGTGTGATGAT
  TGTCCAGGAGATATTAATGCACTTCCAGAATTAAAGCACTATGACCTAAAGCAGCCTACT
  CAGCCTCCTCCACTCCCTGCAGCAGAAGCTGAGAAGCCTGAGTCCTCTGTTTCATCAGCT
  ACTGGAGATGGCAATTTGTCTCCTAGTCAGCCACCTTTAAAGAACCTCTTGTCTCTCCTA
  AAAGCATATTATGCTTTGAATGCACAACCAAGTGCAGAAGAGCTCTCAAAAATTGCTGAT
  TCAGTAAACCTACCACTGGATGTAGTAAAAAAGTGGTTTGAAAAGATGCAAGCTGGACAG
  ATTTCAGTGCAGTCTTCTGAACCATCTTCTCCTGAACCAGGCAAAGTAAATATCCCTGCC
  AAGAACAATGATCAGCCTCAATCTGCAAATGCAAATGAACCCCAGGACAGCACAGTAAAT
  CTACAAAGTCCTTTGAAGATGACTAACTCCCCAGTTTTACCAGTGGGATCAACCACCAAT
  GGTTCCAGAAGTAGTACACCATCCCCATCACCTCTAAACCTTTCCTCATCCAGAAATACA
  CAGGGTTACTTGTACACAGCTGAGGGTGCACAAGAAGAGCCACAAGTAGAACCTCTTGAT
  CTTTCACTACCAAAGCAACAGGGAGAATTATTAGAAAGGTCAACTATCACTAGTGTTTAC
  CAGAACAGTGTTTATTCTGTCCAGGAAGAACCCTTGAACTTGTCTTGCGCAAAAAAGGAG
  CCACAAAAGGACAGTTGTGTTACAGACTCAGAACCAGTTGTAAATGTAATCCCACCAAGT
  GCCAACCCCATAAATATCGCTATACCTACAGTCACTGCCCAGTTACCCACAATCGTGGCC
  ATTGCTGACCAGAACAGTGTTCCATGCTTAAGAGCGCTAGCTGCCAATAAGCAAACGATT
  CTGATTCCCCAGGTGGCATACACCTACTCAACTACGGTCAGCCCTGCAGTCCAAGAACCA
  CCCTTGAAAGTGATCCAGCCAAATGGAAATCAGGATGAAAGACAAGATACTAGCTCAGAA
  GGAGTATCAAATGTAGAGGATCAGAATGACTCTGATTCTACACCGCCCAAAAAGAAAATG
  CGGAAGACAGAAAATGGAATGTATGCTTGTGATTTGTGTGACAAGATATTCCAAAAGAGT
  AGTTCATTATTGAGACATAAATATGAACACACAGGTAAAAGACCTCATGAGTGTGGAATC
  TGTAAAAAGGCATTTAAACACAAACATCATTTGATTGAACACATGCGATTACATTCTGGA
  GAAAAGCCCTATCAATGTGACAAATGTGGAAAGCGCTTCTCACACTCTGGGTCTTATTCT
  CAACACATGAATCATCGCTACTCCTACTGTAAGAGAGAAGCGGAAGAACGTGACAGCACA
  GAGCAGGAAGAGGCAGGGCCTGAAATCCTCTCGAATGAGCACGTGGGTGCCAGGGCGTCT
  CCCTCACAGGGCGACTCGGACGAGAGAGAGAGTTTGACAAGGGAAGAGGATGAAGACAGT
  GAAAAAGAGGAAGAGGAGGAGGATAAAGAGATGGAAGAATTGCAGGAAGAAAAAGAATGT
  GAAAAACCACAAGGGGATGAGGAAGAGGAGGAGGAGGAGGAAGAAGTGGAAGAAGAAGAG
  GTAGAAGAGGCAGAGAATGAGGGAGAAGAAGCAAAAACTGAAGGTCTGATGAAGGATGAC
  AGGGCTGAAAGTCAAGCAAGCAGCTTAGGACAAAAAGTAGGCGAGAGTAGTGAGCAAGTG
  TCTGAAGAAAAGACAAATGAAGCCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001128128
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128128.1, NP_001121600.1
• RefSeq Size: 5667 bp
• RefSeq ORF: 3327 bp
• Locus ID: 6935
• Cytogenetics: 10p11.22
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (1) encodes isoform a.