ROBO2 (NM_001128929) Human Untagged Clone

CAT#: SC323277

ROBO2 (untagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ROBO2
• Synonyms: SAX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001128929, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAGAAGACATGAACGTGTCACTAGAAGGATGTGGACATGGGCTCCGGGACTGTTGATGATGACTG
  TGGTGTTTTGGGGTCATCAGGGGAATGGACAAGGCCAAGGATCGCGTCTTCGCCAGGAGGACTTTCCCCC
  GCGGATTGTGGAGCATCCTTCCGATGTCATCGTCTCTAAGGGCGAGCCCACGACTCTGAACTGCAAGGCG
  GAGGGCCGGCCAACGCCCACCATTGAGTGGTACAAAGATGGGGAGCGAGTGGAGACTGACAAGGACGATC
  CCCGGTCCCACAGGATGCTTCTGCCCAGCGGATCCTTATTCTTCTTGCGCATCGTGCACGGGCGCAGGAG
  TAAACCTGATGAAGGAAGCTACGTTTGTGTTGCGAGGAACTATCTTGGTGAAGCAGTGAGTCGAAATGCG
  TCTCTGGAAGTGGCATTGTTACGAGATGACTTCCGACAAAACCCCACAGATGTTGTAGTGGCAGCTGGAG
  AGCCTGCAATCCTGGAGTGCCAGCCTCCCCGGGGACACCCAGAACCCACCATCTACTGGAAAAAAGACAA
  AGTTCGAATTGATGACAAGGAAGAAAGAATAAGTATCCGTGGTGGAAAACTGATGATCTCCAATACCAGG
  AAAAGTGATGCAGGGATGTATACTTGTGTTGGTACCAATATGGTGGGAGAAAGGGACAGTGACCCAGCAG
  AGCTGACTGTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACCAGGTGGTACTGGAGGAAGAAGC
  TGTAGAATTTCGTTGTCAAGTCCAAGGAGATCCTCAACCAACTGTGAGGTGGAAAAAGGATGATGCAGAC
  TTGCCAAGAGGAAGGTATGACATCAAAGACGATTACACACTAAGAATTAAAAAGACCATGAGTACAGATG
  AAGGCACCTATATGTGTATTGCTGAGAATCGGGTTGGAAAAATGGAAGCCTCTGCTACACTCACCGTCCG
  AGCTCCCCCACAGTTTGTGGTTCGGCCAAGAGATCAGATTGTTGCTCAAGGTCGAACAGTGACATTTCCC
  TGTGAAACTAAAGGAAACCCACAGCCAGCTGTTTTTTGGCAGAAAGAAGGCAGCCAGAACCTACTTTTCC
  CAAACCAACCCCAGCAGCCCAACAGTAGATGCTCAGTGTCACCAACTGGAGACCTCACAATCACCAACAT
  TCAACGTTCCGACGCGGGTTACTACATCTGCCAGGCTTTAACTGTGGCAGGAAGCATTTTAGCAAAAGCT
  CAACTGGAGGTTACTGATGTTTTGACAGATAGACCTCCACCTATAATTCTACAAGGCCCAGCCAACCAAA
  CGCTGGCAGTGGATGGTACAGCGTTACTGAAATGTAAAGCCACTGGTGATCCTCTTCCTGTAATTAGCTG
  GTTAAAGGAGGGATTTACTTTTCCGGGTAGAGATCCAAGAGCAACAATTCAAGAGCAAGGCACACTGCAG
  ATTAAGAATTTACGGATTTCTGATACTGGCACTTATACTTGTGTGGCTACAAGTTCAAGTGGAGAGACTT
  CCTGGAGTGCAGTGCTGGATGTGACAGAGTCTGGAGCAACAATCAGTAAAAACTATGATTTAAGTGACCT
  GCCAGGGCCACCATCCAAACCGCAGGTCACTGATGTTACTAAGAACAGTGTCACCTTGTCCTGGCAGCCA
  GGTACCCCTGGAACCCTTCCAGCAAGTGCATATATCATTGAGGCTTTCAGCCAATCAGTGAGCAACAGCT
  GGCAGACCGTGGCAAACCATGTAAAGACCACCCTCTATACTGTAAGAGGACTGCGGCCCAATACAATCTA
  CTTATTCATGGTCAGAGCGATCAACCCCCAAGGTCTCAGTGACCCAAGTCCCATGTCAGATCCTGTGCGC
  ACACAAGATATCAGCCCACCAGCACAAGGAGTGGACCACAGGCAAGTGCAGAAAGAGCTAGGAGATGTCC
  TTGTCCGTCTTCATAATCCAGTTGTGCTGACTCCCACCACGGTTCAGGTCACATGGACGGTTGATCGCCA
  ACCCCAGTTTATCCAAGGCTACCGAGTGATGTATCGTCAGACTTCAGGTCTGCAGGCGACATCTTCGTGG
  CAGAATTTAGATGCCAAAGTCCCGACTGAACGAAGTGCTGTCTTAGTCAACCTGAAAAAGGGGGTGACTT
  ATGAAATTAAAGTACGGCCATATTTTAATGAGTTCCAAGGAATGGATAGTGAATCTAAAACGGTTCGTAC
  TACTGAAGAAGCCCCAAGTGCCCCACCACAGTCTGTCACTGTACTGACAGTTGGAAGCTACAATAGCACA
  AGTATTAGTGTTTCCTGGGATCCTCCTCCTCCAGATCACCAGAATGGAATTATCCAAGAATACAAGATCT
  GGTGTCTAGGAAATGAAACGCGATTCCATATCAACAAAACTGTGGATGCAGCCATTCGGTCCGTAATAAT
  TGGTGGATTATTCCCAGGTATTCAATACCGGGTAGAGGTTGCAGCTAGTACCAGTGCAGGGGTTGGAGTA
  AAGAGTGAGCCACAGCCAATAATAATCGGGAGACGCAATGAAGTTGTCATTACTGAAAACAATAACAGCA
  TAACTGAGCAAATCACTGATGTGGTGAAGCAACCAGCCTTTATAGCTGGTATTGGTGGTGCCTGCTGGGT
  AATTCTGATGGGTTTTAGCATATGGTTGTATTGGCGAAGAAAGAAGAGGAAGGGACTCAGTAATTATGCT
  GTTACGTTTCAAAGAGGAGATGGAGGACTAATGAGCAATGGAAGCCGTCCAGGTCTTCTCAATGCTGGTG
  ATCCCAGCTATCCATGGCTTGCTGATTCTTGGCCAGCCACGAGCTTGCCAGTAAATAATAGCAACAGTGG
  CCCAAATGAGATTGGAAATTTTGGCCGTGGAGATGTGCTGCCACCAGTTCCAGGCCAAGGGGATAAAACA
  GCAACGATGCTCTCAGATGGAGCCATTTATAGTAGCATTGACTTCACTACCAAAACCAGTTACAACAGTT
  CCAGCCAAATAACACAGGCTACCCCATATGCCACGACACAGATCTTGCATTCCAACAGCATACATGAATT
  GGCTGTCGATCTGCCTGATCCACAATGGAAAAGCTCAATTCAGCAAAAAACAGATCTGATGGGATTTGGT
  TATTCTCTACCTGATCAGAACAAAGGTAACAATGGTGGGAAAGGTGGAAAAAAGAAGAAAAATAAAAACT
  CTTCTAAACCACAGAAAAACAATGGATCCACTTGGGCCAATGTCCCTCTACCTCCCCCCCCAGTCCAGCC
  CCTTCCTGGCACGGAGCTGGAACACTATGCAGTGGAACAACAAGAAAATGGCTATGACAGTGATAGCTGG
  TGCCCACCATTGCCAGTACAAACTTACTTACACCAAGGTCTGGAAGATGAACTGGAAGAAGATGATGATA
  GGGTCCCAACACCTCCTGTTCGAGGCGTGGCTTCTTCTCCTGCTATCTCCTTTGGACAGCAGTCCACTGC
  AACTCTTACTCCATCCCCACGGGAAGAGATGCAACCCATGCTGCAGGCTCACCTGGATGAGTTGACAAGA
  GCCTATCAGTTTGATATAGCAAAACAAACATGGCACATTCAAAGCAATAATCAACCTCCACAGCCTCCAG
  TTCCACCGTTAGGTTATGTGTCTGGAGCCTTGATTTCTGATTTGGAAACGGATGTTGCAGATGATGATGC
  CGACGACGAAGAGGAAGCTTTAGAAATCCCCAGGCCCCTGAGAGCACTGGACCAGACTCCTGGATCCAGC
  ATGGACAATCTAGACAGCTCTGTGACAGGAAAAGCCTTTACCTCCTCTCAAAGACCTCGACCTACCAGCC
  CATTTTCTACTGACAGTAACACCAGTGCAGCCCTGAGTCAAAGTCAGAGGCCTCGGCCCACTAAAAAACA
  CAAGGGAGGGCGGATGGACCAACAACCAGCATTGCCTCATCGAAGGGAAGGAATGACAGATGAGGAGGCC
  TTGGTGCCCTATAGCAAGCCCAGTTTCCCATCTCCAGGTGGCCACAGCTCATCAGGAACAGCTTCTTCTA
  AGGGATCCACTGGACCTAGGAAAACCGAGGTGTTGAGAGCAGGCCACCAGCGCAATGCCAGCGACCTTCT
  TGACATAGGATATATGGGCTCCAACAGTCAAGGACAGTTTACAGGTGAATTATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001128929
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128929.3, NP_001122401.1
• RefSeq Size: 8393 bp
• RefSeq ORF: 4185 bp
• Locus ID: 6092
• Cytogenetics: 3p12.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: 'The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (1) represents the use of an alternate promoter and has multiple differences compared to variant 4. These differences result in a distinct 5' UTR and the use of an alternate start codon compared to variant 4. The encoded protein (isoform ROBO2a; PMID 16829019) has a distinct N-terminus and is shorter than isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.