TBK1 (NM_013254) Human Untagged Clone

CAT#: SC323338

TBK1 (untagged)-Kinase deficient mutant (K38M) of Human TANK-binding kinase 1 (TBK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBK1
• Synonyms: FTDALS4; IIAE8; NAK; T2K
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323338 sequence for NM_013254 edited (data generated by NextGen Sequencing)
  ATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTTAGGCCAAGGAGCTACT
  GCAAATGTCTTTCGTGGAAGACATAAGAAAACTGGTGATTTATTTGCTATCATGGTATTT
  AATAACATAAGCTTCCTTCGTCCAGTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAA
  AAACTCAATCACAAAAATATTGTCAAATTATTTGCTATTGAAGAGGAGACAACAACAAGA
  CATAAAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTATACACTGTTTTAGAAGAA
  CCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGTTTTGCGAGATGTGGTG
  GGTGGAATGAATCATCTACGAGAGAATGGTATAGTGCACCGTGATATCAAGCCAGGAAAT
  ATCATGCGTGTTATAGGGGAAGATGGACAGTCTGTGTACAAACTCACAGATTTTGGTGCA
  GCTAGAGAATTAGAAGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTG
  CACCCTGATATGTATGAGAGAGCAGTGCTAAGAAAAGATCATCAGAAGAAATATGGAGCA
  ACAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGCTACTGGATCACTGCCA
  TTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAGAAGTGATGTATAAAATAATTACAGGA
  AAGCCTTCTGGTGCAATATCTGGAGTACAGAAAGCAGAAAATGGACCAATTGACTGGAGT
  GGAGACATGCCTGTTTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTT
  CTTGCAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTTTTTGCA
  GAAACTAGTGATATACTTCACCGAATGGTAATTCATGTTTTTTCGCTACAACAAATGACA
  GCTCATAAGATTTATATTCATAGCTATAATACTGCTACTATATTTCATGAACTGGTATAT
  AAACAAACCAAAATTATTTCTTCAAATCAAGAACTTATCTACGAAGGGCGACGCTTAGTC
  TTAGAACCTGGAAGGCTGGCACAACATTTCCCTAAAACTACTGAGGAAAACCCTATATTT
  GTAGTAAGCCGGGAACCTCTGAATACCATAGGATTAATATATGAAAAAATTTCCCTCCCT
  AAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGCTAAGGCAATAACAGGG
  GTTGTGTGTTATGCCTGCAGAATTGCCAGTACCTTACTGCTTTATCAGGAATTAATGCGA
  AAGGGGATACGATGGCTGATTGAATTAATTAAAGATGATTACAATGAAACTGTTCACAAA
  AAGACAGAAGTTGTGATCACATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAA
  GTATATGAAAAGTTGATGAAGATCAACCTGGAAGCGGCAGAGTTAGGTGAAATTTCAGAC
  ATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAACAATAGAAACCAGTCTTCAG
  GATATCGACAGCAGATTATCTCCAGGTGGATCACTGGCAGACGCATGGGCACATCAAGAA
  GGCACTCATCCGAAAGACAGAAATGTAGAAAAACTACAAGTCCTGTTAAATTGCATGACA
  GAGATTTACTATCAGTTCAAAAAAGACAAAGCAGAACGTAGATTAGCTTATAATGAAGAA
  CAAATCCACAAATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATGACGCAC
  TTTACAGATGAATGTGTTAAAAAGTATGAGGCATTTTTGAATAAGTCAGAAGAATGGATA
  AGAAAGATGCTTCATCTTAGGAAACAGTTATTATCGCTGACTAATCAGTGTTTTGATATT
  GAAGAAGAAGTATCAAAATATCAAGAATATACTAATGAGTTACAAGAAACTCTGCCTCAG
  AAAATGTTTACAGCTTCCAGTGGAATCAAACATACCATGACCCCAATTTATCCAAGTTCT
  AACACATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAAGGGGTG
  GTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGGCTCTTTAACCATGGAT
  GGTGGCCTTCGCAACGTTGACTGTCTTTAG
  
  Clone variation with respect to NM_013254.3
  113 a=>t;114 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_013254 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATT
  CGGCACGAGGCCCGCCGGCGGTGGCGCGGCGGAGACCCGGCTGGTATAACAAGAGGATTGCCTGATCCAG
  CCAAGATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTTAGGCCAAGGAGCTACTGCAAA
  TGTCTTTCGTGGAAGACATAAGAAAACTGGTGATTTATTTGCTATCATGGTATTTAATAACATAAAGCTT
  CCTTCGTCCAGTGGATGTTCAAATGAGAAGATTTTGAAGTGTTGAAAAAACCTTCAATCCACAAAAATTA
  TTGTCCAAATTATTTTGCTATTTGAAGAAGGGAAACCAACACCAAGAACTTTAAGGTACCTATATTATGG
  AATTTTTGTCCATGGGGGAATTTAAACCCCCGGTTTTTAAAAAAACCCTTTTATGGCTTTATGAACCTAC
  CAGAATCCGGATTTCCTATAATGGTTTTTGCAGGAATGTGGGGGTGGGAAATTGAATCAATTCTCAAAAA
  AAAGGGGTAGATGGCCCCGGGTTCTAACGCCCGAGATATTCCGCGCCGTTTTAAGGGGGAAAAAGGGCCT
  GCTCTGTTTACAAACCTCACAATTTTGTGGTGCCCCTGGAATTTTAAGAAGATGAAGCCCTTTTTTTTTC
  TGTTGTGGGCCCAAGAAAATTTTTCGCCCCCCCTGTGTTTTATAAAAACACCTGTTCCCAGAGATCTTCT
  CTCAAAAATATTTTTGGGACCCCCTGTTATTCTTGTGACACTCTGGGAGACACTTTTATACGCCGCCTCC
  CCGCTGCTCGGCGCTCTATCACCCGCTCTAGGGGCGCGCTCTGGATGATTACGTAGTGATGATATCATTA
  TCTGACAGGACCCTCTCGTGCGCACTCGTGCGAGCAGAACGCACACTAGTGAGCACTAATCGGTCGTG
  
  Kinase domain sequence

  >SC323338 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCCTGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCAC
  GAGGCCCGCCGGCGGTGGCGCGGCGGAGACCCGGCTGGTATAACAAGAGGATTGCCTGATCCAGCCAAGA
  TGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_013254
• ORF Size: 2190 bp
• Insert Size: 2880
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_013254.2, NP_037386.1
• RefSeq Size: 2982
• RefSeq ORF: 2190
• Locus ID: 29110
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]