TSSK1 (TSSK1B) (NM_032028) Human Untagged Clone

CAT#: SC323342

TSSK1B (untagged)-Kinase deficient mutant (K41M) of Human testis-specific serine kinase 1B (TSSK1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSSK1B
• Synonyms: FKSG81; SPOGA4; STK22D; TSK1; TSSK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032028, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATGACGCTGCTGTCCTCAAGCGACGAGGCTACCTCCTGGGGATAAATTTAGGAGAGGGCTCCTATG
  CAAAAGTAAAATCTGCTTACTCTGAGCGCCTGAAGTTCAATGTGGCGATCAAGATCATCGACCGCAAGAA
  GGCCCCCGCAGACTTCTTGGAGAAATTCCTTCCCCGGGAAATTGAGATTCTGGCCATGTTAAACCACTGC
  TCCATCATTAAGACCTACGAGATCTTTGAGACATCACATGGCAAGGTCTACATCGTCATGGAGCTCGCGG
  TCCAGGGCGACCTCCTCGAGTTAATCAAAACCCGGGGAGCCCTGCATGAGGACGAAGCTCGCAAGAAGTT
  CCACCAGCTTTCCTTGGCCATCAAGTACTGCCACGACCTGGACGTCGTCCACCGGGACCTCAAGTGTGAC
  AACCTTCTCCTTGACAAGGACTTCAACATCAAGCTGTCCGACTTCAGCTTCTCCAAGCGCTGCCTGCGGG
  ATGACAGTGGTCGAATGGCATTAAGCAAGACCTTCTGTGGGTCACCAGCGTATGCGGCCCCAGAGGTGCT
  GCAGGGCATTCCCTACCAGCCCAAGGTGTACGACATCTGGAGCCTAGGCGTGATCCTCTACATCATGGTC
  TGCGGCTCCATGCCCTACGACGACTCCAACATCAAGAAGATGCTGCGTATCCAGAAGGAGCACCGCGTCA
  ACTTCCCACGCTCCAAGCACCTGACAGGCGAGTGCAAGGACCTCATCTACCACATGCTGCAGCCCGACGT
  CAACCGGCGGCTCCACATCGACGAGATCCTCAGCCACTGCTGGATGCAGCCCAAGGCACGGGGATCTCCC
  TCTGTGGCCATCAACAAGGAGGGGGAGAGTTCCCGGGGAACTGAACCCTTGTGGACCCCCGAACCTGGCT
  CTGACAAGAAGTCTGCCACCAAGCTGGAGCCTGAGGGAGAGGCACAGCCCCAGGCACAGCCTGAGACAAA
  ACCCGAGGGGACAGCAATGCAAATGTCCAGGCAGTCGGAGATCCTGGGTTTCCCCAGCAAGCCGTCGACT
  ATGGAGACAGAGGAAGGGCCCCCCCAACAGCCTCCAGAGACGCGGGCCCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_032028 unedited
  CCCGCCCGTTCTCAGCAAAGGGCGGTAGGCGATGTACGGATGTGTGAGGCTCTATATAAGCAGAGCTCGT
  TTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACAT
  TATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGGGGGCAGGAACAG
  CAGGCCTGGCCAGCCCAAAGGACTCTCTATCCAGGATGTAAATGAGCACACTGCTGGCCCATGCGCCTCG
  GGGCTGTAGAGGCAGCCTCAGAGCACTGGGCATTCCTGGCACCATGGATGACGCTGCTGGTCCCTCAGGC
  CGAGCGAATGCCTACCTTCCTGGGGGATAAATTTTAGGAGAAGGGCTCCTATGGCAAAAGTAAATTCTGC
  TTACTCGGGACGGCCCGGAAGTTTCATGGGGGGGGATCAGGGATCATCGACCCGCAAAAGGGCCCCCCGC
  GAACTTCTTTGGAAAAATTTCCTTTCCCGGGGAAATTGAAATTTTTGGCCCGGTTAAACCCCGGGCCCCT
  CCTTTAAAAACTACAAAATTTTTTGAAAACTTCAATGGGAGGGGTCAAATTCCCCCAGGGACCCCCGGGC
  CCAGGGCAACCCCCCTAAATTTAACCAAAACCGGGGGGACCCCCGGGAAGAGGAAAAACCCCCAAAAAAT
  TTCCCCCCTTTTTTTTTGTCCCCAAATATTTTCGCCCACCCCCGGAGTCTTTCCCCCCGGGGCCCCATTT
  TGGAAACCCTTTTTTTTTTGGGGGGGTTTTCATCAAAGGTTGGAATATTTTTTTTTTTAAACCCCCCTGC
  GGGAGGAAAAAGTGGGAAAAGGGTTATAAAAAAATTCTTTGGTTGCCCCCCATGTCGCCCCCCACGGGGG
  GTTCGGGGGTTTCTTCCCTCCCCCCGGTGTGGGACACTCGTGGCACGGCTGAGTGCTCTCTATTGGTGGG
  GGGGCGGCGCCCCCGCACAAGACACTACAAAGAAAGAGCGTATGATGAGAGAAGGAGAGAGCGACTTCTT
  TCCTGTATCGTACACT
  
  Kinase domain sequence

  >SC323342 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSATGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATG
  GATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGGGGGCAGGAACAGCAGGCCTGGCCAGC
  CCAAAGGACTCTCTATCCAGGATGTAAATGAGCACACTGCTGGCC
  
• Restriction Sites: Please inquire
• ACCN: NM_032028
• ORF Size: 1104 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_032028.2, NP_114417.1
• RefSeq Size: 2012
• RefSeq ORF: 1104
• Locus ID: 83942
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: TSSK1 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]). [supplied by OMIM, Mar 2008]