PRAK (MAPKAPK5) (NM_003668) Human Untagged Clone

CAT#: SC323359

MAPKAPK5 (untagged)-Kinase deficient mutant (K45M) of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPKAPK5
• Synonyms: MAPKAP-K5; MK-5; MK5; PRAK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323359 sequence for NM_003668 edited (data generated by NextGen Sequencing)
  ATGTCGGAGGAGAGCGACATGGACAAAGCCATCAAGGAAACTTCCATTTTAGAAGAATAC
  AGTATCAATTGGACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGTTAGAGTCTGTGTA
  AAGAAATCTACTCAAGAACGGTTTGCGCTGAWRATTCTTCTTGATCGTCCAAAAGCTAGA
  AATGAGGTACGTCTGCACATGATGTGTGCCACACACCCAAACATAGTTCAGATTATTGAA
  GTGTTTGCTAACAGTGTCCAGTTTCCCCATGAGTCCAGCCCTAGGGCCCGACTCTTAATT
  GTAATGGAGATGATGGAAGGGGGAGAGCTATTTCACAGAATCAGCCAGCACCGGCACTTT
  ACAGAGAAGCAAGCCAGCCAAGTAACAAAGCAGATAGCTTTGGCTCTGCGGCACTGTCAC
  TTGTTAAACATTGCGCACAGAGACCTCAAGCCTGAAAATCTGCTTTTTAAGGATAACTCT
  TTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTG
  ATGACACCCCAGTTCACCCCTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGG
  CATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGACGCCCTACACTTACAACAAG
  AGCTGTGACTTGTGGTCCCTAGGGGTGATTATCTATGTGATGCTGTGCGGATACCCTCCT
  TTTTACTCCAAACACCACAGCCGGACTATCCCAAAGGATATGCGAAGAAAGATCATGACA
  GGCAGTTTTGAGTTCCCAGAGGAAGAGTGGAGTCAGATCTCAGAGATGGCCAAAGATGTT
  GTGAGGAAGCTCCTGAAGGTCAAACCGGAGGAGAGACTCACCATCGAGGGAGTGCTGGAC
  CACCCCTGGCTCAATTCCACCGAGGCCCTGGATAATGTGCTGCCTTCTGCTCAGCTGATG
  ATGGACAAGGCAGTGGTTGCAGGAATCCAGCAGGCTCACGCGGAACAGTTGGCCAACATG
  AGAATCCAGGATCTGAAAGTCAGCCTCAAACCCCTGCACTCAGTGAACAACCCCATTCTG
  CGGAAGAGGAAGTTACTTGGCACCAAGCCAAAGGACAGTGTCTATATCCACGACCATGAG
  AATGGAGCCGAGGATTCCAATGTTGCCTTGGAAAAACTCCGAGATGTGATTGCTCAGTGT
  ATTCTCCCCCAGGCTGGAGAGAATGAAGATGAGAAACTGAATGAAGTAATGCAGGAGGCT
  TGGAAGTATAACCGGGAATGCAAACTCCTAAGAGATACTCTGCAGAGCTTCAGCTGGAAT
  GGTCGTGGATTCACAGATAAAGTAGATCGACTAAAACTGGCAGAAATTGTGAAGCAGGTG
  ATAGAAGAGCAAACCACGTCCCACGAATCCCAATAA
  
  Clone variation with respect to NM_003668.2
  152 a=>w;153 a=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003668 unedited
  TCGGCACAAAGGAACAACGAGCGGAGGCGCGCACGGGAGAGCCAACACAAGCAGAGCACGACAAGCGAAC
  CGACAGAATACGCAACACGACACACCACAGGGCGGCCGCGAACCCGGCACGAGGGCCGAGCCCCCCGCCC
  CCCCGGCCGCGCGGGGACAGGGCTGCTGAGCAGCCTCCGCCTCTCCCGGCTGTGGGGGCCCCACTGAGTA
  TGTCGGAGGAGAGCGACATGGACAAAGCCATCAAGGAAACTTCCATTTTAGAAGAATACAGTATCAATTG
  GACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGTTAGAGTCTGTGTAAAGAAATCTACTCAAGAACGG
  TTTGCGCTGATGATTCTTCTTGATCGTCCAAAAGCTAGAACGGTTTGCGCTGATGATTCTTCTTGATCGT
  CCAAAAGCTAGAACGGTTTGCGCTGATGATTCTTCTTGATCGTCCAAAGCTAGAACGGTTTGCGCTGATG
  ATTCTCTGATCGTCCAAAGCTAGACGGTTTGCGCTGATGATCTCTGATCGTCCAAAGCTAGACGGTTGCG
  CTGATGATCTCTGATCGTCAAAGCTAAACGGTTTGCGCTGATGATTCTTCCTGATCGTCAAAGCTAAACG
  GTTGCGCCTGATGATCCTTCTGATTCGGTCAAGCTAAATGAGGTACGTCTGGCATGAAGTGTGGCCCACA
  CCAACTAGTTCAATATATGGAATGTTTGGCTACGTGCAGTTTCCATGAGTCAGCCTTAGGCGATCTTATT
  GTATTGGATGATTGAGAGGGGGAGCAATTCATGATCTGACGCGGGCTTACGTAAGCAGCACCGCGTATCA
  ACGATAGCTTGTCTGCGCAGTCTGCACTTGTTAC
  
  Kinase domain sequence

  >SC323359 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AWATTTTAGAGWAAGTATCAATTGGACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGTTAGAGTCTGT
  GTAAAGAAATCTACTCAAGAACGGTTTGCGCTGATGATTCTTCTTGATCGTCCAAAAGCTAGAACGGTTT
  GCGCTGATGATTCTTCTTGATCGTCCAAAAGCTAGAACGGTTTGCGCTGATGATTCTTCTTGATCGTCCA
  AAAGCTAGAACGGTTTGCGCTGATGATTCTTCTTGATCGTCCAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_003668
• ORF Size: 1416 bp
• Insert Size: 1900
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003668.2, NP_003659.2
• RefSeq Size: 2060
• RefSeq ORF: 1416
• Locus ID: 8550
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (1) is the more frequently occurring transcript.