MAPK6 (NM_002748) Human Untagged Clone

CAT#: SC323360

MAPK6 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase 6 (MAPK6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPK6
• Synonyms: ERK3; HsT17250; p97MAPK; PRKM6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323360 sequence for NM_002748 edited (data generated by NextGen Sequencing)
  ATGGCAGAGAAATTTGAAAGTCTCATGAACATTCATGGTTTTGATCTGGGTTCTAGGTAT
  ATGGACTTAAAACCATTGGGTTGTGGAGGCAATGGCTTGGTTTTTTCTGCTGTAGACAAT
  GACTGTGACAAAAGAGTAGCCATCATGAAAATTGTCCTTACTGATCCCCAGAGTGTCAAA
  CATGCTCTACGTGAAATCAAAATTATTAGAAGACTTGACCATGATAACATTGTGAAAGTG
  TTTGAGATTCTTGGTCCCAGTGGAAGCCAATTAACAGACGATGTGGGCTCTCTTACGGAA
  CTGAACAGTGTTTACATTGTTCAGGAGTACATGGAGACAGACTTGGCTAATGTGCTGGAG
  CAGGGCCCTTTACTGGAAGAGCATGCCAGGCTTTTCATGTATCAGCTGCTACGGGGGCTC
  AAGTATATTCACTCTGCAAATGTACTGCACAGAGATCTCAAACCAGCTAATCTTTTCATT
  AATACGGAAGACTTGGTGCTGAAGATAGGTGACTTTGGTCTTGCACGGATCATGGATCCT
  CATTATTCCCATAAGGGTCATCTTTCTGAAGGATTGGTTACTAAATGGTACAGATCTCCA
  CGTCTTTTACTTTCTCCTAATAATTATACTAAAGCCATTGACATGTGGGCTGCAGGCTGC
  ATCTTTGCTGAAATGCTGACTGGTAAAACCCTTTTTGCAGGTGCACATGAACTTGAACAG
  ATGCAGCTGATTTTAGAATCTATTCCTGTTGTACATGAGGAAGATCGTCAGGAGCTTCTC
  AGCGTAATTCCAGTTTACATTAGAAATGACATGACTGAGCCACACAAACCTTTAACTCAG
  CTGCTTCCAGGAATTAGTCGAGAAGCAGTGGATTTCCTGGAACAAATTTTGACATTTAGC
  CCCATGGATCGGTTAACAGCAGAAGAAGCACTCTCCCATCCTTACATGAGCATATATTCT
  TTTCCAATGGATGAGCCAATTTCAAGCCATCCTTTTCATATTGAAGATGAAGTTGATGAT
  ATTTTGCTTATGGATGAAACTCACAGTCACATTTATAACTGGGAAAGGTATCATGATTGT
  CAGTTTTCAGAGCATGATTGGCCTGTACATAACAACTTTGATATTGATGAAGTTCAGCTT
  GATCCAAGAGCTCTGTCCGATGTCACTGATGAAGAAGAAGTACAAGTTGATCCCCGAAAA
  TATTTGGATGGAGATCGGGAAAAGTATCTGGAGGATCCTGCTTTTGATACCAATTACTCT
  ACTGAGCCTTGTTGGCAATACTCAGATCATCATGAAAACAAATATTGTGATCTGGAGTGT
  AGCCATACTTGTAACTACAAAACGAGGTCATCATCATATTTAGATAACTTAGTTTGGAGA
  GAGAGTGAAGTTAACCATTACTATGAACCCAAGCTTATTATAGATCTTTCCAATTGGAAA
  GAACAAAGCAAAGAAAAATCTGATAAGAAAGGCAAATCAAAATGTGAAAGGAATGGATTG
  GTTAAAGCCCAGATAGCGCTAGAGGAAGCATCACAGCAACTGGCTGGAAAAGAAAGGGAA
  AAGAATCAGGGATTTGATTTTGATTCCTTTATTGCAGGAACTATTCAGCTTAGTTCCCAG
  CATGAGCCTACTGATGTTGTTGATAAATTAAATGACTTGAATAGCTCAGTGTCCCAACTA
  GAATTGAAAAGTTTGATATCAAAGTCAGTAAGCCAAGAAAAACAGGAAAAAGGAATGGCA
  AATCTGGCTCAATTAGAAGCCTTGTACCAGTCTTCTTGGGACAGCCAGTTTGTGAGTGGT
  GGGGAGGACTGTTTTTTCATAAATCAGTTTTGTGAGGTAAGGAAGGATGAACAAGTTGAG
  AAGGAAAACACTTACACTAGTTACTTGGACAAGTTCTTTAGCAGGAAAGAAGATACTGAA
  ATGCTAGAAACTGAGCCAGTAGAGGATGGGAAGCTTGGGGAGAGAGGACATGAGGAAGGA
  TTTCTGAACAACAGTGGGGAGTTCCTCTTTAACAAGCAGCTCGAGTCCATAGGCATCCCA
  CAGTTTCACAGTCCAGTTGGGTCACCACTTAAGTCAATACAGGCCACATTAACACCTTCT
  GCTATGAAATCTTCCCCTCAAATTCCTCATCAAACATACAGCAGCATTCTGAAACATCTG
  AACTAA
  
  Clone variation with respect to NM_002748.3
  146 a=>t;868 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002748 unedited
  AAGGAAGAGGGCGAGAAGACGACGTGGGGGAGGGAGGGCAACAGCAGGCAGGCAGCGAAAGTAGACAACG
  ACAGAAACAAAGAGGGAGGAGATGGACGAGGCGACGGGAAGCGAGAGCCCGGGAAGCAGAGCACCGCAAG
  CAAAGAACAGAGTAAAACAGAAGAGCCAGAAGCTTAGAAACACACGAAGAGGACGGGGAAGACCGAAGGA
  CAAAGGAAGAAGGGAGGGAGCCCCGACAGGGAAAGGGGGGGGGCGGGGGCCACCAGGACACCAAACAAAG
  AAAACCCACGGCAAACCAAACAGCCGGCAGGGGCCCCCGGCGAAAAAAAAAAAAAACCACCAAGGGAAAA
  AAAGAAACCAAAACGGAAAAAGCGGCCAACAGCCCCTCCAGAAAGGAGGCCGCCTCAGGACCCGTTCCAC
  ACTGGATGGTTTTTGCACTGTTGAGGAAAAAGGATATTAGAAGCCTGGTTGGTGTGCATATTTTATTTCA
  CATTTTTTGTTAAATGTTAAATCGTTTTAGCACGGTAATCTGAGTTGCACAGTATGTCATTTCATTTCCG
  TTTTGAGTTTTCTTTGTTTTCGTTAAATGTCTGCAGAGTTGCTGCCCCTTTCTTGAACTATGAGTACTGC
  AATCTTTTTAATTCTCAATATGAATAGAGCTTTTTGAGCTTTAAATCTAAGGGGAACTCGACAGGCCTGT
  TTGGCATATGCAATGAACATCAAGAAACCATCTTGCTGTGGAAGCATAATTATTTTTCTTCTCCTTTTGA
  AGATCTTTCCTTTGATGCCAGTTTTCTTCTTGTTACACCAAGTTCATTTGAAAGAAAAGCATAGTAAGTT
  TCAAATGCAGAGAATTTGAAGTCTCATGACATCATGTTTGATCTGGTTCTAGTATATGACTAAACATTGG
  TTGTGGAGGCATTGCCT
  
  Kinase domain sequence

  >SC323360 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AGAATTAACATTGGGTTGTGGAGGCATGGCTTGGTTTTTTCTGCTGTAGACAATGACTGTGACAAAAGAG
  TAGCCATCATGAAAATTGTCCTTACTGATCCCCAGAGTGTCAAACATGCTCTACGTGAAATCAAAATTAT
  TAGAAGACTTGACCATGATAACATTGTGAAAGTGTTTGAGATTCTTGGTCCCAGTGGAAGCCAATTAACA
  GACGATGTGGGCTCTCTTACGGAACTGAACAGTGTTTACATTGTT
  
• Restriction Sites: Please inquire
• ACCN: NM_002748
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002748.2, NP_002739.1
• RefSeq Size: 4193 bp
• RefSeq ORF: 2166 bp
• Locus ID: 5597
• Cytogenetics: 15q21.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Gene Summary: 'The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]'