PFTK1 (CDK14) (NM_012395) Human Untagged Clone

CAT#: SC323368

CDK14 (untagged)-Kinase deficient mutant (K164M) of Human PFTAIRE protein kinase 1 (PFTK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK14
• Synonyms: PFTAIRE1; PFTK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012395, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCACGGTTACTTTGGCTGCAATGCTGCTGCAGAGCCCGGTTACTCTGCCTTCGTGGGAACTCCACAGA
  TATGTGTCACAAAGATGTCTACACGGAACTGCCAGGGAATGGACTCAGTGATCAAACCCCTGGACACAAT
  TCCTGAGGATAAAAAAGTCAGAGTTCAGAGGACACAGAGCACTTTTGACCCATTTGAGAAACCAGCTAAT
  CAAGTAAAGAGGGTGCATTCTGAGAACAATGCTTGCATTAACTTTAAGACCTCCTCCACTGGCAAAGAGT
  CACCTAAAGTTAGGCGGCACTCCAGCCCCAGCTCGCCAACAAGTCCCAAATTTGGAAAAGCTGACTCATA
  TGAAAAGCTGGAAAAACTAGGGGAAGGATCTTATGCTACAGTATACAAAGGGAAAAGCAAGGTAAATGGG
  AAGTTGGTAGCTCTGAAGGTGATCAGGCTGCAGGAAGAAGAAGGGACACCTTTCACAGCTATCAGGGAAG
  CTTCTCTTTTAAAAGGACTAAAACATGCTAACATAGTGCTACTTCATGACATCATCCATACCAAGGAGAC
  GCTGACACTTGTGTTTGAATATGTGCACACTGATTTATGTCAGTACATGGACAAGCACCCTGGGGGGCTG
  CATCCAGATAATGTGAAGTTGTTTTTATTTCAGTTGCTGCGAGGTCTGTCTTACATCCACCAGCGTTATA
  TTTTGCACAGAGACCTGAAACCACAGAACCTTCTGATCAGTGACACGGGGGAGTTAAAGCTGGCAGATTT
  CGGTCTTGCAAGAGCAAAATCCGTCCCTAGCCACACATACTCCAACGAAGTGGTTACCTTGTGGTACAGA
  CCTCCAGATGTCCTTCTAGGCTCAACAGAATATTCCACCTGCCTTGACATGTGGGGAGTAGGTTGCATCT
  TTGTTGAAATGATCCAAGGAGTTGCTGCTTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGAAT
  ATTTCTGGTTCTTGGAACACCAAATGAGGACACATGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCA
  GAACGCTTTACCCTGTACAGCTCTAAAAACCTTAGACAAGCATGGAATAAGCTCAGCTATGTGAACCATG
  CAGAGGACCTGGCCTCCAAGCTCCTACAATGTTCCCCAAAGAACAGACTGTCGGCACAGGCTGCCTTGAG
  CCACGAGTATTTTAGTGACCTGCCGCCACGGCTATGGGAACTCACCGACATGTCTTCTATTTTTACTGTC
  CCAAATGTGAGATTGCAACCAGAAGCTGGAGAAAGCATGCGGGCCTTTGGGAAAAACAATAGTTATGGCA
  AAAGTCTATCAAACAGCAAGCACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_012395 unedited
  CCCGCCCGTCTCAGCAATGGGCGGTAGGCGTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGGTTGTAG
  TACGGCCGTACCATTTCAGCTTGCTAGTGCAGAAAGATGTGAATTCAGTTGCTGTATGAGCCTGGCCTGG
  TGCAAACACATTGCTAGAGACATGTTAAAGAGTTCCAGGTGAATCAAGCCTGAGGGAGACAACAGCAAAA
  CGATATGTGTCACAAAGATGTCTACACGGAACTGCCAGGGGAATGGACTCAGTGATCAAACCCCTGGACA
  CAATTCCTGAGGATTAAAAAAAGTCAGAGTTTCAGAGGACACAGAGCCACTTTTTGACCCATTGAAGAAA
  CCCAGCTTAATCAAGTTAAAGAGGGTGGCATTCGAAAAACAAGGCTTGGCATTACCTTTAGAACCTCCCC
  AACTGCCAAGAAGTCACCTAAGGTTAGGCGGACTTCAGCCCCAGCTCGGCCACAGGGTCCCAATTGGAAA
  AGCGCTGACTATATAGAAAAAGCTGGGAAAACTTAGGGGGAGGGATTATGCCCACGTATACAAAAGGAAA
  AAAACAGTGAATGGGAGATTTTGTAGTCTGAGGTGATATCGCGCTGCGAGAAGAAAGGACACTTTTACAC
  ACTATCGGGGACTCTCTTTAAAGAGCAACACGCTAACATGGCATCTTTGAACTCTCTACCAGGGACGTGA
  CCTTGTGTGGAATGTGCCACCTGATATTGCTACCTGACAACCCTGGGCCGACTCAGAATTGGAGTGCTAT
  CATTGCGCAAGCTGCCTATACCACGCTATATGCGAACTGACCAGGCTGTCATAGCAGGGGATAAGCGCG
  
  Kinase domain sequence

  >SC323368 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ACKATGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGGTTGTAGTACGGCCG
  TACCATTTCAGCTTGCTAGTGCAGAAAGATGTGAATTCAGTTGCTGTATGAGCCTGGCCTGGTGCAAACA
  CATTGCTAGAGACATGTTAAAGAGTTCCAGGTGAATCAAGCCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_012395
• ORF Size: 1356 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_012395.2, NP_036527.1
• RefSeq Size: 4953
• RefSeq ORF: 1356
• Locus ID: 5218
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: PFTK1 is a member of the CDC2 (MIM 116940)-related protein kinase family (Yang and Chen, 2001 [PubMed 11313143]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a.