CAMK2D (NM_172115) Human Untagged Clone

CAT#: SC323377

CAMK2D (untagged)-Kinase deficient mutant (K43M) of Human calcium/calmodulin-dependent protein kinase II delta (CAMK2D), transcript variant 4


  "NM_172115" in other vectors (5)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CAMK2D
Synonyms CAMKD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_172115, the custom clone sequence may differ by one or more nucleotides


ATGGCTTCGACCACAACCTGCACCAGGTTCACGGACGAGTATCAGCTTTTCGAGGAGCTTGGAAAGGGGG
CATTCTCAGTGGTGAGAAGATGTATGAAAATTCCTACTGGACAAGAATATGCTGCCAAAATTATCAACAC
CAAAAAGCTTTCTGCTAGGGATCATCAGAAACTAGAAAGAGAAGCTAGAATCTGCCGTCTTTTGAAGCAC
CCTAATATTGTGCGACTTCATGATAGCATATCAGAAGAGGGCTTTCACTACTTGGTGTTTGATTTAGTTA
CTGGAGGTGAACTGTTTGAAGACATAGTGGCAAGAGAATACTACAGTGAAGCTGATGCCAGTCATTGTAT
ACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAG
AATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAG
TTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACG
TAAAGATCCTTATGGAAAGCCAGTGGATATGTGGGCATGTGGTGTCATTCTCTATATTCTACTTGTGGGG
TATCCACCCTTCTGGGATGAAGACCAACACAGACTCTATCAGCAGATCAAGGCTGGAGCTTATGATTTTC
CATCACCAGAATGGGACACGGTGACTCCTGAAGCCAAAGACCTCATCAATAAAATGCTTACTATCAACCC
TGCCAAACGCATCACAGCCTCAGAGGCACTGAAGCACCCATGGATCTGTCAACGTTCTACTGTTGCTTCC
ATGATGCACAGACAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCA
TCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGT
AAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATT
ATCAAAGTCACTGAACAACTGATCGAAGCTATCAACAATGGGGACTTTGAAGCCTACACAAAAATCTGTG
ACCCAGGCCTTACTGCTTTTGAACCTGAAGCTTTGGGTAATTTAGTGGAAGGGATGGATTTTCACCGATT
CTACTTTGAAAATGCTTTGTCCAAAAGCAATAAACCAATCCACACTATTATTCTAAACCCTCATGTACAT
CTGGTAGGGGATGATGCCGCCTGCATAGCATATATTAGGCTCACACAGTACATGGATGGCAGTGGAATGC
CAAAGACAATGCAGTCAGAAGAGACTCGTGTGTGGCACCGCCGGGATGGAAAGTGGCAGAATGTTCATTT
TCATCGCTCGGGGTCACCAACAGTACCCATCAAGTAA


>OriGene 5' read for mutant NM_172115 unedited
CCCGCCGTCTCAGCAATGGGCGGTAGGCGTGTACGGATGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGCCGCGCGTCTGCT
CGCTTCTGGTCCGTCGCGCTCCCAGCCAGGGCACAGCCCGGACCGAGGATGGCTTCGACCACAACCTGCA
CCAGGTTCACGGACGAGTATCAGCTTTTCGAGGAGCTTGGAAAGGGGGCATTCTCAGTGGTGAGAAGATG
TATGAAAATTCCTACTGGACAAGAATATGCTGCCATGATTATCAACACCAAAAAGCTTTTCTGGTGGGGT
GATCGGTCAGAAAACTAGAAAAGAGAAAGCTAGAAATCTTGCCGTCTTTTTGAACAACCCCTAAAATTGT
GCCGAACGTTCATGGATAGCATATCGAAGAAGGGCTTTCACTACTTGGTGGTTTGGATTTAGTTCCGGGA
GGTGAACGGTTTGAAAAATTGTGGGGCAGAAAAATACTACGGGGAAGCTGAGGCCAGCCTTGGTAACAGC
GAATTCTAGAAGTTGTTAATATTGGTCACCTATGGGCAAAGTTCCAAGGGACCTAGAGCTGGGAAATTTT
GTTTAGCTATCCAAATCAAGGGGACCCGCGTGTAATTGGGCCACACTTGGGCTATCCCATAGATTTCAGG
GAGCCCCCGCGTGTGTTGTGTTTGTCGGGCCCCGGAATCTTCTCTCCAAATTTTCCTAGATCTCTTGAAC
CCCGTGTATTGTGCACGTGGTGACATCAAAAACATGTGGGGATACACTCTGGGAAAACACAATAATCACC
GCTATGCGAGGAGTCGAGGTGTCATACGAAGACGAGCGTATGCATGAGCAC
>SC323377 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGCCGCGCGTCTGCTCGCTTC
TGGTCCGTCGCGCTCCCAGCCAGGGCACAGCCCGGACCGAGGATGGCTTCGACCACAACCTGCACCAGGT
TCACGGACGAGTATCAGCTTTTCGAGGAGCTTGGAAAGGGGGCAT
Restriction Sites Please inquire     
ACCN NM_172115
Insert Size 4500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172115.1, NP_742113.1
RefSeq Size 2151 bp
RefSeq ORF 1437 bp
Locus ID 817
Cytogenetics 4q26
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway
Gene Summary 'The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.[provided by RefSeq, Nov 2008]'
Transcript Variant: This variant (4) has multiple differences in the 3' coding region, compared to variant 3. The resulting isoform (1) has a shorter C-terminus, compared to isoform 3. Isoform 1 is encoded by transcript variants 1 and 4.

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