STK23 (SRPK3) (NM_014370) Human Untagged Clone

CAT#: SC323379

SRPK3 (untagged)-Kinase deficient mutant (K108M) of Human SFRS protein kinase 3 (SRPK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRPK3
• Synonyms: MSSK-1; MSSK1; STK23
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014370, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGCGGCAGTAGCAGCAGCTCACAGGCCT
  CCTGCGGGCCCGAGTCCTCGGGCTCCGAACTAGCCCTGGCCACACCGGTGCCTCAGATGCTGCAGGGCCT
  TCTGGGCTCCGACGACGAGGAACAGGAAGACCCCAAAGACTACTGCAAGGGCGGCTACCACCCTGTGAAG
  ATCGGCGACGTGTTCAATGGGCGGTACCACGTGGTGCGCAAACTGGGCTGGGGCCACTTCTCCACCGTCT
  GGCTCTGCTGGGACATCCAGCGCAAGCGCTTTGTGGCCCTCAAAGTGGTGAAGAGTGCGGGGCATTACAC
  GGAGACAGCTGTGGATGAGATCAAGCTCCTGAAATGTGTCCGGGACAGCGACCCCAGTGACCCCAAAAGA
  GAGACCATTGTCCAGCTCATTGATGACTTCAGGATCTCAGGAGTCAATGGAGTCCATGTGTGCATGGTGC
  TGGAGGTGCTGGGCCACCAGCTCCTCAAATGGATCATCAAGTCCAACTACCAGGGCCTGCCCGTGCCCTG
  CGTGAAGAGCATCGTGAGGCAGGTGCTGCACGGCCTGGACTACCTCCACACCAAGTGCAAGATCATCCAC
  ACGGACATCAAGCCCGAGAACATCTTGCTGTGTGTGGGGGACGCTTACATCAGGCGCCTGGCTGCCGAGG
  CCACGGAGTGGCAACAGGCAGGGGCGCCGCCCCCCTCCCGCTCCATAGTCAGCACTGCCCCCCAGGAGGT
  CTTGCAGACCGGTAAGCTGTCCAAAAACAAGAGGAAGAAGATGAGGCGCAAACGGAAACAGCAGAAGCGG
  CTGCTGGAGGAGCGGCTGCGGGACCTGCAGAGGCTGGAGGCCATGGAGGCTGCCACCCAGGCTGAGGACT
  CTGGCTTGAGACTAGACGGGGGCAGCGGCTCCACATCCTCTTCAGGCTGTCACCCCGGGGGCGCCAGAGC
  AGGTCCCTCCCCAGCCTCTTCCTCCCCCGCCCCAGGGGGCGGCCGTAGCCTCAGCGCGGGCTCACAGACC
  TCAGGCTTCTCCGGCTCCCTCTTCTCTCCTGCCTCCTGCTCCATCCTCTCCGGCTCGTCCAATCAGCGAG
  AGACCGGGGGCCTCCTGTCGCCTAGCACACCATTCGGTGCCTCGAACCTCCTGGTGAACCCCCTGGAGCC
  CCAAAATGCAGATAAGATCAAGATCAAGATCGCAGACCTGGGCAACGCCTGCTGGGTGCACAAGCACTTC
  ACGGAAGACATCCAGACTCGGCAGTACCGGGCCGTCGAGGTGCTGATCGGCGCCGAATACGGCCCCCCGG
  CAGACATCTGGAGCACAGCCTGCATGGCCTTCGAGCTGGCCACTGGTGACTACCTGTTCGAGCCGCATTC
  TGGAGAAGACTACAGTCGTGATGAGGACCACATCGCTCACATAGTGGAGCTTCTGGGGGACATCCCCCCA
  GCCTTCGCCCTCTCAGGCCGCTATTCCCGGGAGTTCTTCAACCGGAGAGGAGAGCTGCGGCACATCCACA
  ATCTCAAGCACTGGGGCCTGTACGAGGTACTCATGGAAAAGTACGAGTGGCCCCTAGAGCAGGCCACACA
  GTTCAGCGCCTTTCTGCTGCCCATGATGGAGTACATCCCCGAAAAGCGGGCCAGTGCCGCTGACTGCCTC
  CAGCACCCCTGGCTCAACCCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014370 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGCCCGGGGCAC
  CGGAGCTGCGGGCTGCGTGGCCGGGATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGC
  GGCAGTAGCAGCAGCTCACAGGCCTCCTGCGGGCCCGAGTCCTCGGGCTCCGAACTAGCCCTGGCCACAC
  CGGTGCCTCAGATGCTGCAGGGGCCTTTCTGGGCTCCGACGACGAGGGAACAGGGAAAGACCCCAAAGAA
  CTACTGCAAGGGCGGGCTACCCACCCCTGTTGAAAGAATCGGGCGACGTGGTTCATGGGGGCGGGTCCCA
  CGTGGGTGGGGGCAAAACTGGGCCTGGGGCCATTTTCTCCACCCGTCCTGGCTTGGCCTGGGACATCCCA
  CCGCCAGGCCCCTTTGGGGCCCCTCATTTTGTTAAAAAATTCCGGGCCATTTACACGGAAAAGGTTTGGG
  GAAAAAAATTAACCCCCCGAAAATTGGTCCCGGAAACCCGAACCCCCTGGGCCCCAAAAAAAAAAAAACA
  TTTGGCCCATCTATTTGGGAAATTTTCGGGTTTCCCGGGTTTTAAGGGGGCCCCGGGGGGCCCAGGGCCC
  CGAAAATCTCCTCCCCCCCCCCCCCCTAAAGGGGTATTATAGTTGCCACTTCAACAGGGCCCTTCCCTTC
  CCCCGGGTAGAAAAATACTCAGGGGGCGAGGGTGCTCGACGCTCTGGAACTCCCCCACCCCATTTGTGCG
  ATATTTTCTCCCGCGGAGGTATTCGCCACGAGAAACTTGTTGAGGGTGGGGGGGATCATAATATAGGGAC
  GCGTGATAGTGGCGACCGCAGATGATACAGAGAGGCGGGAGCCCCTCTCACATGATGCTAGACTCATTCT
  CTGCAAGGGAGATTG
  
  Kinase domain sequence

  >SC323379 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGCCCGGGGCACCGGAGC
  TGCGGGCTGCGTGGCCGGGATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGCGGCAGT
  AGCAGCAGCTCACAGGCCTCCTGCGGGCCCGAGTCCTCGGGCTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_014370
• ORF Size: 1704 bp
• Insert Size: 2300
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_014370.2, NP_055185.2
• RefSeq Size: 2040
• RefSeq ORF: 1704
• Locus ID: 26576
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).