CKII alpha (CSNK2A1) (NM_001895) Human Untagged Clone

CAT#: SC323395

CSNK2A1 (untagged)-Kinase deficient mutant (K68M) of Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2


  "NM_001895" in other vectors (7)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CSNK2A1
Synonyms CK2A1; Cka1; Cka2; CKII; OCNDS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323395 sequence for NM_001895 edited (data generated by NextGen Sequencing)
ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCT
CGAGAATACTGGGATTACGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAG
CTGGTTCGAAAATTAGGCCGAGGTAAATACAGTGAAGTATTTGAAGCCATCAACATCACA
AATAATGAAAAAGTTGTTGTTATGATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGT
GAAATAAAGATTTTGGAGAATTTGAGAGGAGGTCCCAACATCATCACACTGGCAGACATT
GTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTTTTTGAACACGTAAACAACACAGAC
TTCAAGCAATTGTACCAGACGTTAACAGACTATGATATTCGATTTTACATGTATGAGATT
CTGAAGGCCCTGGATTATTGTCACAGCATGGGAATTATGCACAGAGATGTCAAGCCCCAT
AATGTCATGATTGATCATGAGCACAGAAAGCTACGACTAATAGACTGGGGTTTGGCTGAG
TTTTATCATCCTGGCCAAGAATATAATGTCCGAGTTGCTTCCCGATACTTCAAAGGTCCT
GAGCTACTTGTAGACTATCAGATGTACGATTATAGTTTGGATATGTGGAGTTTGGGTTGT
ATGCTGGCAAGTATGATCTTTCGGAAGGAGCCATTTTTCCATGGACATGACAATTATGAT
CAGTTGGTGAGGATAGCCAAGGTTCTGGGGACAGAAGATTTATATGACTATATTGACAAA
TACAACATTGAATTAGATCCACGTTTCAATGATATCTTGGGCAGACACTCTCGAAAGCGA
TGGGAACGCTTTGTCCACAGTGAAAATCAGCACCTTGTCAGCCCTGAGGCCTTGGATTTC
CTGGACAAACTGCTGCGATATGACCACCAGTCACGGCTTACTGCAAGAGAGGCAATGGAG
CACCCCTATTTCTACACTGTTGTGAAGGACCAGGCTCGAATGGGTTCATCTAGCATGCCA
GGGGGCAGTACGCCCGTCAGCAGCGCCAATATGATGTCAGGGATTTCTTTAGTGCCAACC
CCTTCACCCCTTGGACCTCTGGCAGGCTCACCAGTGATTGCTGCTGCCAACCCCCTTGGG
ATGCCTGTTCCAGCTGCCGCTGGCGCTCAGCAGTAA

Clone variation with respect to NM_001895.3
203 a=>t;204 a=>g;1070 c=>t
>OriGene 5' read for mutant NM_001895 unedited
CCGCCCGTCCAGCAACGGGCGGTAGGCGCGTACGGCGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGAGCGGCCGCC
GCCGCTGCCGCTTCCACCACAGTTTGAAGAAAACAGGTCTGAAACAAGGTCTTACCCCCAGCTGCTTCTG
AACACAGTGACTGCCAGATCTCCAAACATCAAGTCCAGCTTTGTCCGCCAACCTGTCTGACATGTCGGGA
CCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCTCGAGAATACTGGTGATTAC
TGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCCAGCTGGTTCGAAAATTAGGCCGAGGTGA
ATTACAGTTGAAGTATTTGAAGCCCATCACATCACAAATAATGAAAAAGTTGTTGGTTATGATTCTCAGC
CAGTAAAAAAGAAGAAAATTAGCGTGAAATAAGATTTGGAGATTTGGAAGGAGGTCCCAACTCATCACCT
GCAGGACATTGAAAAGACCTGGGTAAGAACCCCCGCCTGGTTTTTGAACCGTAACCACCGATTCAGCATT
GTCCAACTTACGACATGGATTCGATTTACGGTAAATCGAGCCTGATATGCCAACTGGATTAGCAGAATTC
AGCCTATGTCGATGACTGGCCGGAGACCACTAGACTGGTGCGATTAAACTGCCAATATGCAAGTTCGGAC
TCAGTCGACATGACTGATGCAATTGATTGATGGGCGGCATTTT
>SC323395 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CKCTCGAATAGGCCGAGGTAATACAGTGAAGTATTTGAAGCCATCAACATCACAAATAATGAAAAAGTTG
TTGTTATGATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTGAG
AGGAGGTCCCAACATCATCACACTGGCAGACATTGTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTT
TTTGAACACGTAAACAACACAGACTTCAAGCAATTGTACCAGACG
Restriction Sites Please inquire     
ACCN NM_001895
Insert Size 2680 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001895.3, NP_001886.1
RefSeq Size 2732 bp
RefSeq ORF 1176 bp
Locus ID 1457
Cytogenetics 20p13
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
Protein Pathways Adherens junction, Tight junction, Wnt signaling pathway
Gene Summary 'Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Apr 2018]'
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 4 and 5 encode the same protein.

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