STK16 (NM_003691) Human Untagged Clone

CAT#: SC323397

STK16 (untagged)-Kinase deficient mutant (K49M) of Human serine/threonine kinase 16 (STK16), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK16
• Synonyms: FLJ39635; KRCT; KRCT, MPSK, TSF1, PKL12, FLJ39635; MPSK; PKL12; protein kinase expressed in day 12 fetal liver; serine/threonine kinase 16; TSF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323397 sequence for NM_003691 edited (data generated by NextGen Sequencing)
  ATGGGCCACGCGCTGTGTGTCTGCTCTCGGGGAACTGTCATCATTGACAATAAGCGCTAC
  CTCTTCATCCAGAAACTGGGGGAGGGTGGGTTCAGCTATGTGGACCTAGTGGAAGGGTTA
  CATGATGGACACTTCTACGCCCTGATGCGAATCCTGTGTCACGAGCAGCAGGACCGGGAG
  GAGGCCCAGCGAGAAGCCGACATGCATCGCCTCTTCAATCACCCCAACATCCTTCGCCTC
  GTGGCTTACTGTCTGAGGGAACGGGGTGCTAAGCATGAGGCCTGGCTGCTGCTACCATTC
  TTCAAGAGAGGTACGCTGTGGAATGAGATAGAAAGGCTGAAGGACAAAGGCAACTTCCTG
  ACCGAGGATCAAATCCTTTGGCTGCTGCTGGGGATCTGCAGAGGCCTTGAGGCCATTCAT
  GCCAAGGGTTATGCCCACAGAGACTTGAAGCCCACCAATATATTGCTTGGAGATGAGGGG
  CAGCCAGTTTTAATGGACTTGGGTTCCATGAATCAAGCATGCATCCATGTGGAGGGCTCC
  CGCCAGGCTCTGACCCTGCAGGACTGGGCAGCCCAGCGGTGCACCATCTCCTACCGAGCC
  CCAGAGCTCTTCTCTGTGCAGAGTCACTGTGTCATCGATGAGCGGACTGATGTCTGGTCC
  CTAGGCTGCGTGCTATATGCCATGATGTTTGGGGAAGGCCCTTATGACATGGTGTTCCAA
  AAGGGTGACAGTGTGGCCCTTGCTGTGCAGAACCAACTCAGCATCCCACAAAGCCCCAGG
  CATTCTTCAGCATTGTGGCAGCTCCTGAACTCGATGATGACCGTGGACCCGCATCAGCGT
  CCTCACATTCCTCTCCTCCTCAGTCAGCTGGAGGCGCTGCAGCCCCCAGCTCCTGGCCAA
  CATACTACCCAAATCTGA
  
  Clone variation with respect to NM_003691.2
  146 a=>t;796 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003691 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGCGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTCTCTCTGGCCC
  GAGCCAGCATGATCCGCTGGGCCCCCAGCGCATCTCCTGGAAGAGCCCACTCACCCTGGACGAGCTCTTC
  GGTAGCCTCAGACCGTCCTTGAAGAGGATGACTGAGACATTATGGGCCACGCGCTGTGTGTCTGCTCTCG
  GGGAACTGTCATCATTGACAATAAGCGCTACCTCTTCATCCAGAAACTGGGGGGAGGGTGGGGTTTCTGC
  TATTGTGGACCTAGTGGAAGGGTTACATGATGGGACACTTTCTACCGCCCCTGATGCGAATCTGGTGTCC
  ACGAAGCAGCAGGACCCGGGAGGAAGGCCCAGCGAAGAAGCCGACATGGCATCCGCCCTCTTCATTCACC
  CCAACCATCCTTCGCCCCTGGTGGCCTTACTGTCTAGAGGGAACGGGGTGCTAAGCATGAGGCCTGCCTG
  CTGCTCCCATTCTTAAAAAAAGGTACCCGGGGGAATGAGATAAAAAGGGCTAAAGAGACAAAGGCCATTT
  CCTGACGAGGGATCAAACTCTTTGGTCTGGCGTGTGGGGATTCGCAGAGCCCTGTGAGGCCATCTCGCCC
  AGGGTATATGCCACAGACATGTAGACCCACACTATATTTTGCTGGATATGAGGCGCCACCTTTATAGGGC
  ACGTGGTCTCGAGATACACTCTGCATCGTGTAGAGGCTCCCACGTCTACACCTGATGTGAACACAGTGTG
  CACTATCCTAGACCAGAGCTTTTGTGACACTCGTGTCCATAGACGAGTTGTGCCACATGCGTCTCTACCA
  GTTGGAAGC
  
  Kinase domain sequence

  >SC323397 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSACGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTCTCTCTGGCCCGAGCC
  AGCATGATCCGCTGGGCCCCCAGCGCATCTCCTGGAAGAGCCCACTCACCCTGGACGAGCTCTTCGGTAG
  CCTCAGACCGTCCTTGAAGAGGATGACTGAGACATTATGGGCCAC
  
• Restriction Sites: Please inquire
• ACCN: NM_003691
• ORF Size: 918 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003691.2, NP_003682.2
• RefSeq Size: 1682
• RefSeq ORF: 918
• Locus ID: 8576
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase