Casein Kinase 1 delta (CSNK1D) (NM_139062) Human Untagged Clone

CAT#: SC323403

CSNK1D (untagged)-Kinase deficient mutant (K38M) of Human casein kinase 1, delta (CSNK1D), transcript variant 2


  "NM_139062" in other vectors (7)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CSNK1D
Synonyms ASPS; CKI-delta; CKId; CKIdelta; FASPS2; HCKID
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323403 sequence for NM_139062 edited (data generated by NextGen Sequencing)
ATGGAGCTGAGAGTCGGGAACAGGTACCGGCTGGGCCGGAAGATCGGCAGCGGCTCCTTC
GGAGACATCTATCTCGGTACGGACATTGCTGCAGGAGAAGAGGTTGCCATCATGCTTGAA
TGTGTCAAAACCAAACACCCTCAGCTCCACATTGAGAGCAAAATCTACAAGATGATGCAG
GGAGGAGTGGGCATCCCCACCATCAGATGGTGCGGGGCAGAGGGGGACTACAACGTCATG
GTGATGGAGCTGCTGGGGCCAAGCCTGGAGGACCTCTTCAACTTCTGCTCCAGGAAATTC
AGCCTCAAAACCGTCCTGCTGCTTGCTGACCAAATGATCAGTCGCATCGAATACATTCAT
TCAAAGAACTTCATCCACCGGGATGTGAAGCCAGACAACTTCCTCATGGGCCTGGGGAAG
AAGGGCAACCTGGTGTACATCATCGACTTCGGGCTGGCCAAGAAGTACCGGGATGCACGC
ACCCACCAGCACATCCCCTATCGTGAGAACAAGAACCTCACGGGGACGGCGCGGTACGCC
TCCATCAACACGCACCTTGGAATTGAACAATCCCGAAGAGATGACTTGGAGTCTCTGGGC
TACGTGCTAATGTACTTCAACCTGGGCTCTCTCCCCTGGCAGGGGCTGAAGGCTGCCACC
AAGAGACAGAAATACGAAAGGATTAGCGAGAAGAAAATGTCCACCCCCATCGAAGTGTTG
TGTAAAGGCTACCCTTCCGAATTTGCCACATACCTGAATTTCTGCCGTTCCTTGCGTTTT
GACGACAAGCCTGACTACTCGTACCTGCGGCAGCTTTTCCGGAATCTGTTCCATCGCCAG
GGCTTCTCCTATGACTACGTGTTCGACTGGAACATGCTCAAATTTGGTGCCAGCCGGGCC
GCCGATGACGCCGAGCGGGAGCGCAGGGACCGAGAGGAGCGGCTGAGACACTCGCGGAAC
CCGGCTACCCGCGGCCTCCCTTCCACAGCCTCCGGCCGCCTGCGGGGGACGCAGGAAGTG
GCTCCCCCCACACCCCTCACCCCTACCTCACACACGGCTAACACCTCCCCCCGGCCCGTC
TCCGGCATGGAGAGAGAGCGGAAAGTGAGTATGCGGCTGCACCGCGGGGCCCCCGTCAAC
ATCTCCTCGTCCGACCTCACAGGCCGACAAGATACCTCTCGCATGTCCACCTCACAGAAT
AGCATTCCTTTCGAACACCACGGCAAGTAG

Clone variation with respect to NM_139062.2
113 a=>t
>OriGene 5' read for mutant NM_139062 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTCCTTTAGGC
AGCTGAAAGGGGATTTAGGCCCGGAAGATCCGAGTCCATCCGCGGCGGGGAGAGGGCAAGCGGGACCGGT
AGGGGCCGGAGCAGCGGCGGCGGCGCTCGGACTGTCCCATCCGCCCCGTATTGAGGCGCTGGGAGCGGCG
GGGCGACAGGAAAGCGATGGTGAAAGCGGGGCCGTGAGGGGGGGCGGGAGCCGGGGAGCCGGGACCTGTA
GTATCGGCAGCAGCGGCGCCCGCCTCCCCAGAAGTTCAGAACCCAGGAGCCGGCCGGGGAGGGGAGGGAG
CGGAATCCGGGCCGCCGCCCGCCTGGGAGCCTTAAAATTCGGGACAAGGAACCGGCGGGGCCGGAGAAAT
CGGCACCGGTTCCTTCGGAGCATTCTTATCTCGGTACGACATTGGCTGCAGGAAAAAAGGTTGCATCATG
CTGGATGGGTGTCAAAACCAAAACCTCTGGGCTCCACTTGGAGCGCAAATTCACAATGTAGTGCGGGGGG
GGTGTGGGACTCCCACTAACAAAGGGCGGGGCGCAAGGGGGATCACCCTGTTAGGGGAGGGGCTCGGTGG
GGACACTCTGAAAGCC
>SC323403 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSAATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTCCTTTAGGCAGCT
GAAAGGGGATTTAGGCCCGGAAGATCCGAGTCCATCCGCGGCGGGGAGAGGGCAAGCGGGACCGGTAGGG
GCCGGAGCAGCGGCGGCGGCGCTCGGACTGTCCCATCCGCCCCGT
Restriction Sites Please inquire     
ACCN NM_139062
Insert Size 2500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_139062.1, NP_620693.1
RefSeq Size 2106 bp
RefSeq ORF 1230 bp
Locus ID 1453
Cytogenetics 17q25.3
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Circadian rhythm - mammal, Gap junction, Hedgehog signaling pathway
Gene Summary 'This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]'
Transcript Variant: This variant (2) has an additional exon at the 3' end compared to transcript variant 1. This results in a shorter isoform (2) with a different C-terminus compared to isoform 1.

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