CaMKI (CAMK1) (NM_003656) Human Untagged Clone

CAT#: SC323415

CAMK1 (untagged)-Kinase deficient mutant (K49M) of Human calcium/calmodulin-dependent protein kinase I (CAMK1)


  "NM_003656" in other vectors (7)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CAMK1
Synonyms CAMKI
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323415 sequence for NM_003656 edited (data generated by NextGen Sequencing)
ATGCTGGGGGCAGTGGAAGGCCCCAGGTGGAAGCAGGCGGAGGACATTAGAGACATCTAC
GACTTCCGAGATGTTCTGGGCACGGGGGCCTTCTCGGAGGTGATCCTGGCAGAAGATAAG
AGGACGCAGAAGCTGGTGGCCATCATGTGCATTGCCAAGGAGGCCCTGGAGGGCAAGGAA
GGCAGCATGGAGAATGAGATTGCTGTCCTGCACAAGATCAAGCACCCCAACATTGTAGCC
CTGGATGACATCTATGAGAGTGGGGGCCACCTCTACCTCATCATGCAGCTGGTGTCGGGT
GGGGAGCTCTTTGACCGTATTGTGGAAAAAGGCTTCTACACGGAGCGGGACGCCAGCCGC
CTCATCTTCCAGGTGCTGGATGCTGTGAAATACCTGCATGACCTGGGCATTGTACACCGG
GATCTCAAGCCAGAGAATCTGCTGTACTACAGCCTGGATGAAGACTCCAAAATCATGATC
TCCGACTTTGGCCTCTCCAAGATGGAGGACCCGGGCAGTGTGCTCTCCACCGCCTGTGGA
ACTCCGGGATACGTGGCCCCTGAAGTCCTGGCCCAGAAGCCCTACAGCAAGGCTGTGGAT
TGCTGGTCCATAGGTGTCATCGCCTACATCTTGCTCTGCGGTTACCCTCCCTTCTATGAC
GAGAATGATGCCAAACTCTTTGAACAGATTTTGAAGGCCGAGTACGAGTTTGACTCTCCT
TACTGGGACGACATCTCTGACTCTGCCAAAGATTTCATCCGGCACTTGATGGAGAAGGAC
CCAGAGAAAAGATTCACCTGTGAGCAGGCCTTGCAGCACCCATGGATTGCAGGAGATACA
GCTCTAGATAAGAATATCCACCAGTCGGTGAGTGAGCAGATCAAGAAGAACTTTGCCAAG
AGCAAGTGGAAGCAAGCCTTCAATGCCACGGCTGTGGTGCGGCACATGAGGAAACTGCAG
CTGGGCACCAGCCAGGAGGGGCAGGGGCAGACGGCGAGCCATGGGGAGCTGCTGACACCA
GTGGCTGGGGGGCCGGCAGCTGGCTGTTGCTGTCGAGACTGCTGCGTGGAGCCGGGCACA
GAACTGTCCCCCACACTGCCCCACCAGCTCTAG

Clone variation with respect to NM_003656.4
146 a=>t;147 a=>g
>OriGene 5' read for mutant NM_003656 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCAGGCGGGCGG
AGAGAGCCGCCGAGCCGAGCCGAGCCCCAGCTCCAGCAAGAGCGCGGGCGGGTGGCCCAGGCACGCAGCG
GTGAGGACCGCGGCCACAGCTCGGCGCCAACCACCGCGGGCCTCCCAGCCAGCCCCGCGGCGGGGCAGCC
GCAGGGAGCCCTGGCTGTGGGTCGGGGGGGCAGTGGGGCCATGCTGGGGGGCAGTGGAAAGGCCCCGGTT
GGGTTCTTGGCGGAGGACTTTTTAGACATCTCCAACTTCGAAAATGTTCCTGGGCACGGGGGCGTTGGGG
AGGGTGATCCTGGCAAAGATAAAAGGACCCCAAACCGGTGGGCCTTAAGTGCAATGGCCAAGGAGGCCTG
GAGGGCCAGAAAGCCC
>SC323415 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSAAGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCAGGCGGGCGGAGAGA
GCCGCCGAGCCGAGCCGAGCCCCAGCTCCAGCAAGAGCGCGGGCGGGTGGCCCAGGCACGCAGCGGTGAG
GACCGCGGCCACAGCTCGGCGCCAACCACCGCGGGCCTCCCAGCC
Restriction Sites Please inquire     
ACCN NM_003656
ORF Size 1113 bp
Insert Size 1770
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Reference Data
RefSeq NM_003656.3, NP_003647.1
RefSeq Size 1501
RefSeq ORF 1113
Locus ID 8536
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]

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