PIM2 (NM_006875) Human Untagged Clone

CAT#: SC323418

PIM2 (untagged)-Kinase deficient mutant (K61M) of Human pim-2 oncogene (PIM2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIM2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323418 sequence for NM_006875 edited (data generated by NextGen Sequencing)
  ATGTTGACCAAGCCTCTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCA
  GGAGGCAAGGATCGGGAAGCGTTCGAGGCCGAGTATCGACTCGGCCCCCTCCTGGGTAAG
  GGGGGCTTTGGCACCGTCTTCGCAGGACACCGCCTCACAGATCGACTCCAGGTGGCCATC
  AYGGTGATTCCCCGGAATCGTGTGCTGGGCTGGTCCCCCTTGTCAGACTCAGTCACATGC
  CCACTCGAAGTCGCACTGCTATGGAAAGTGGGTGCAGGTGGTGGGCACCCTGGCGTGATC
  CGCCTGCTTGACTGGTTTGAGACACAGGAGGGCTTCATGCTGGTCCTCGAGCGGCCTTTG
  CCCGCCCAGGATCTCTTTGACTATATCACAGAGAAGGGCCCACTGGGTGAAGGCCCAAGC
  CGCTGCTTCTTTGGCCAAGTAGTGGCAGCCATCCAGCACTGCCATTCCCGTGGAGTTGTC
  CATCGTGACATCAAGGATGAGAACATCCTGATAGACCTACGCCGTGGCTGTGCCAAACTC
  ATTGATTTTGGTTCTGGTGCCCTGCTTCATGATGAACCCTACACTGACTTTGATGGGACA
  AGGGTGTACAGCCCCCCAGAGTGGATCTCTCGACACCAGTACCATGCACTCCCGGCCACT
  GTCTGGTCACTGGGCATCCTCCTCTATGACATGGTGTGTGGGGACATTCCCTTTGAGAGG
  GACCAGGAGATTCTGGAAGCTGAGCTCCACTTCCCAGCCCATGTCTCCCCAGACTGCTGT
  GCCCTAATCCGCCGGTGCCTGGCCCCCAAACCTTCTTCCCGACCCTCACTGGAAGAGATC
  CTGCTGGACCCCTGGATGCAAACACCAGCCGAGGATGTACCCCTCAACCCCTCCAAAGGA
  GGCCCTGCCCCTTTGGCCTGGTCCTTGCTACCCTAA
  
  Clone variation with respect to NM_006875.3
  182 a=>y;183 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_006875 unedited
  ACCGCCGTTCAGCAATGGGCGGTAGGCGTGTACGGCGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGATGTTGACCAAGCCT
  CTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCAGGAGGCAAGGATCGGGAAGCGTTCG
  AGGCCGAGTATCGACTCGGCCCCCTCCTGGGTAAGGGGGGCTTTGGCACCGTCTTCGCAGGACACCGCCT
  CACAGATCGACTCCAGGTGGCCATCATGGTGATTCCCCGGAATCGTGTGCTGGGCTGGTCGCTGCTTTGT
  GTGTCTCAGTCACATGCCCACTCGAAGTCGCACTGCTATGGAAAGTGGGTGCAGGTGGTGGGCACCCTGC
  GTGATCCGCCTGCTTGACTGTTTGAGACACAGGAGGCTTCATGCTGGTCCTCGAGCGCCTTTGCCCGCCC
  AGGATTCTCTTTGACTTATATCCACAGAGAAGCCCACTGGGTGAAGCCCAAGCCGCTGCTTCTTGCAAGT
  ATGGCAGCATCAGCACTGCATTCCATGAAGTTGTCATCGTGACTCAAGATGAAAACCATCTTGATAGACC
  TACGGCTGGGCTGTGCAAATCTTATTGGATTTGTTTCTGGTGCTCTGCTTCGTGATGAACCTTACCTTGA
  CTTGAGTGGCACAGGGTGTCACGGCCCCAATGTGTATTCTTGGACACAGATACATAGTCATCCGGACAGT
  TGTGATATTGACTCCTTAAGAGTGTGTGGGACATTCCTTTAGAGCACAAGAATTCGAGAATCAGTATCTT
  ATCTAGCACACAGTGTCCAATGCTTGTGCCTATACGGTGCTGCGCGCCCAACTTCGACACTGGAATCGTG
  TGCAACCGTATTACACCC
  
  Kinase domain sequence

  >SC323418 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSACTGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGATGTTGACCAAGCCTCTACA
  GGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCAGGAGGCAAGGATCGGGAAGCGTTCGAGGCC
  GAGTATCGACTCGGCCCCCTCCTGGGTAAGGGGGGCTTTGGCACC
  
• Restriction Sites: Please inquire
• ACCN: NM_006875
• ORF Size: 936 bp
• Insert Size: 2000
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_006875.2, NP_006866.2
• RefSeq Size: 2101
• RefSeq ORF: 936
• Locus ID: 11040
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia
• Gene Summary: This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival. [provided by RefSeq, Nov 2009]