SYK (NM_003177) Human Untagged Clone

CAT#: SC323420

SYK (untagged)-Kinase deficient mutant (K402M) of Human spleen tyrosine kinase (SYK), transcript variant 1


  "NM_003177" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SYK
Synonyms p72-Syk
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323420 sequence for NM_003177 edited (data generated by NextGen Sequencing)
ATGGCCAGCAGCGGCATGGCTGACAGCGCCAACCACCTGCCCTTCTTTTTCGGCAACATC
ACCCGGGAGGAGGCAGAAGATTACCTGGTCCAGGGGGGCATGAGTGATGGGCTTTATTTG
CTGCGCCAGAGCCGCAACTACCTGGGTGGCTTCGCCCTGTCCGTGGCCCACGGGAGGAAG
GCACACCACTACACCATCGAGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGG
ACCCATGCCAGCCCCGCCGACCTCTGCCACTACCACTCCCAGGAGTCTGATGGCCTGGTC
TGCCTCCTCAAGAAGCCCTTCAACCGGCCCCAAGGGGTGCAGCCCAAGACTGGGCCCTTT
GAGGATTTGAAGGAAAACCTCATCAGGGAATATGTGAAGCAGACATGGAACCTGCAGGGT
CAGGCTCTGGAGCAGGCCATCATCAGTCAGAAGCCTCAGCTGGAGAAGCTGATCGCTACC
ACAGCCCATGAAAAAATGCCTTGGTTCCATGGAAAAATCTCTCGGGAAGAATCTGAGCAA
ATTGTCCTGATAGGATCAAAGACAAATGGAAAGTTCCTGATCCGAGCCAGAGACAACAAC
GGCTCCTACGCCCTGTGCCTGCTGCACGAAGGGAAGGTGCTGCACTATCGCATCGACAAA
GACAAGACAGGGAAGCTCTCCATCCCCGAGGGAAAGAAGTTCGACACGCTCTGGCAGCTA
GTCGAGCATTATTCTTATAAAGCAGATGGTTTGTTAAGAGTTCTTACTGTCCCATGTCAA
AAAATCGGCACACAGGGAAATGTTAATTTTGGAGGCCGTCCACAACTTCCAGGTTCCCAT
CCTGCGACTTGGTCAGCGGGTGGAATAATCTCAAGAATCAAATCATACTCCTTCCCAAAG
CCTGGCCACAGAAAGTCCTCCCCTGCCCAAGGGAACCGGCAAGAGAGTACTGTGTCATTC
AATCCGTATGAGCCAGAACTTGCACCCTGGGCTGCAGACAAAGGCCCCCAGAGAGAAGCC
CTACCCATGGACACAGAGGTGTACGAGAGCCCCTACGCGGACCCCGAGGAGATCAGGCCC
AAGGAGGTTTACCTGGACCGAAAGCTGCTGACGCTGGAAGACAAAGAACTGGGCTCTGGT
AATTTTGGAACTGTGAAAAAGGGCTACTACCAAATGAAAAAAGTTGTGAAAACCGTGGCT
GTGATGATACTGAAAAACGAGGCCAATGACCCCGCTCTTAAAGATGAGTTATTAGCAGAA
GCAAATGTCATGCAGCAGCTGGACAACCCGTACATCGTGCGGATGATCGGGATATGCGAG
GCCGAGTCCTGGATGCTGGTTATGGAGATGGCAGAACTTGGTCCCCTCAATAAGTATTTG
CAGCAGAACAGACATGTCAAGGATAAGAACATCATAGAACTGGTTCATCAGGTTTCCATG
GGCATGAAGTACTTGGAGGAGAGCAATTTTGTGCACAGAGATCTGGCTGCAAGAAATGTG
TTGCTAGTTACCCAACATTACGCCAAGATCAGTGATTTCGGACTTTCCAAAGCACTGCGT
GCTGATGAAAACTACTACAAGGCCCAGACCCATGGAAAGTGGCCTGTCAAGTGGTACGCT
CCGGAATGCATCAACTACTACAAGTTCTCCAGCAAAAGCGATGTCTGGAGCTTTGGAGTG
TTGATGTGGGAAGCATTCTCCTATGGGCAGAAGCCATATCGAGGGATGAAAGGAAGTGAA
GTCACCGCTATGTTAGAGAAAGGAGAGCGGATGGGGTGCCCTGCAGGGTGTCCAAGAGAG
ATGTACGATCTCATGAATCTGTGCTGGACATACGATGTGGAAAACAGGCCCGGATTCGCA
GCAGTGGAACTGCGGCTGCGCAATTACTACTATGACGTGGTGAACTAA

Clone variation with respect to NM_003177.5
1205 a=>t;1206 a=>g
>OriGene 5' read for mutant NM_003177 unedited
ACCGCCCGTCTGAGCAACGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGT
GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGGAACGAC
GCGCTGCTTTCATGCCCTTTCTTGTTCTACCTTCATCGGCCGAGGTAAAAGTGCTGAAACCATGTGAATA
AAATACAGGTGGACACCTGCGCAGGTGTGTGCCCTCCGGCCCCTGAAGCATGGCCAGCAGCGGCATGGCT
GACAGCGCCAACCACCTGCCCTTCTTTTTCTGCAACATCACCCCGGGAGGAGGCATAAGATTACCTGGGT
GGAGGTGGGGCATGAGTGATGGGCTTAATTAGGTTCCCCATAAGCCGGAATTCCCAGTGGGGGGGGAGGC
GGGGGCCTCCGGCCCGAAAGAGGGGGCCTGCCATTCCCTGCCGTGGCTTAAATTTTGCGGGGGGAGGGTC
CGGAGAA
>SC323420 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GACTGGGCTCTGGTATTTTGGACTGTGAAAAGGGCTACTACCAAATGAAAAAAGTTGTGAAAACCGTGGC
TGTGATGATACTGAAAAACGAGGCCAATGACCCCGCTCTTAAAGATGAGTTATTAGCAGAAGCAAATGTC
ATGCAGCAGCTGGACAACCCGTACATCGTGCGGATGATCGGGATATGCGAGGCCGAGTCCTGGATGCTGG
TTATGGAGATGGCAGAACTTGGTCCCCTCAATAAGTATTTGCAGC
Restriction Sites Please inquire     
ACCN NM_003177
Insert Size 2660 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003177.3, NP_003168.2
RefSeq Size 2672 bp
RefSeq ORF 1908 bp
Locus ID 6850
Cytogenetics 9q22.2
Domains pkinase, SH2, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity
Gene Summary 'This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (Syk(L)). Both variants 1 and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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