DDR2 (NM_006182) Human Untagged Clone
CAT#: SC323423
DDR2 (untagged)-Kinase deficient mutant (K608M) of Human discoidin domain receptor tyrosine kinase 2 (DDR2), transcript variant 2
"NM_006182" in other vectors (7)
Product Images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | DDR2 |
Synonyms | MIG20a; NTRKR3; TKT; TYRO10; WRCN |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC323423 sequence for NM_006182 edited (data generated by NextGen Sequencing)
ATGATCCTGATTCCCAGAATGCTCTTGGTGCTGTTCCTGCTGCTGCCTATCTTGAGTTCT GCAAAAGCTCAGGTTAATCCAGCTATATGCCGCTATCCTCTGGGCATGTCAGGAGGCCAG ATTCCAGATGAGGACATCACAGCTTCCAGTCAGTGGTCAGAGTCCACAGCTGCCAAATAT GGAAGGCTGGACTCAGAAGAAGGGGATGGAGCCTGGTGCCCTGAGATTCCAGTGGAACCT GATGACCTGAAGGAGTTTCTGCAGATTGACTTGCACACCCTCCATTTTATCACTCTGGTG GGGACCCAGGGGCGCCATGCAGGAGGTCATGGCATCGAGTTTGCCCCCATGTACAAGATC AATTACAGTCGGGATGGCACTCGCTGGATCTCTTGGCGGAACCGTCATGGGAAACAGGTG CTGGATGGAAATAGTAACCCCTATGACATTTTCCTAAAGGACTTGGAGCCGCCCATTGTA GCCAGATTTGTCCGGTTCATTCCAGTCACCGACCACTCCATGAATGTGTGTATGAGAGTG GAGCTTTACGGCTGTGTCTGGCTAGATGGCTTGGTGTCTTACAATGCTCCAGCTGGGCAG CAGTTTGTACTCCCTGGAGGTTCCATCATTTATCTGAATGATTCTGTCTATGATGGAGCT GTTGGATACAGCATGACAGAAGGGCTAGGCCAATTGACCGATGGTGTGTCTGGCCTGGAC GATTTCACCCAGACCCATGAATACCACGTGTGGCCCGGCTATGACTATGTGGGCTGGCGG AACGAGAGTGCCACCAATGGCTACATTGAGATCATGTTTGAATTTGACCGCATCAGGAAT TTCACTACCATGAAGGTCCACTGCAACAACATGTTTGCTAAAGGTGTGAAGATCTTTAAG GAGGTACAGTGCTACTTCCGCTCTGAAGCCAGTGAGTGGGAACCTAATGCCATTTCCTTC CCCCTTGTCCTGGATGACGTCAACCCCAGTGCTCGGTTTGTCACGGTGCCTCTCCACCAC CGAATGGCCAGTGCCATCAAGTGTCAATACCATTTTGCAGATACCTGGATGATGTTCAGT GAGATCACCTTCCAATCAGATGCTGCAATGTACAACAACTCTGAAGCCCTGCCCACCTCT CCTATGGCACCCACAACCTATGATCCAATGCTTAAAGTTGATGACAGCAACACTCGGATC CTGATTGGCTGCTTGGTGGCCATCATCTTTATCCTCCTGGCCATCATTGTCATCATCCTG TGGAGGCAGTTCTGGCAGAAAATGCTGGAGAAGGCTTCTCGGAGGATGCTGGATGATGAA ATGACAGTCAGCCTTTCCCTGCCAAGTGATTCTAGCATGTTCAACAATAACCGCTCCTCA TCACCTAGTGAACAAGGGTCCAACTCGACTTACGATCGCATCTTTCCCCTTCGCCCTGAC TACCAGGAGCCATCCAGGCTGATACGAAAACTCCCAGAATTTGCTCCAGGGGAGGAGGAG TCAGGCTGCAGCGGTGTTGTGAAGCCAGTCCAGCCCAGTGGCCCTGAGGGGGTGCCCCAC TATGCAGAGGCTGACATAGTGAACCTCCAAGGAGTGACAGGAGGCAACACATACTCAGTG CCTGCCGTCACCATGGACCTGCTCTCAGGAAAAGATGTGGCTGTGGAGGAGTTCCCCAGG AAACTCCTAACTTTCAAAGAGAAGCTGGGAGAAGGACAGTTTGGGGAGGTTCATCTCTGT GAAGTGGAGGGAATGGAAAAATTCAAAGACAAAGATTTTGCCCTAGATGTCAGTGCCAAC CAGCCTGTCCTGGTGGCTGTGATGATGCTCCGAGCAGATGCCAACAAGAATGCCAGGAAT GATTTTCTTAAGGAGATAAAGATCATGTCTCGGCTCAAGGACCCAAACATCATCCATCTA TTAGCTGTGTGTATCACTGATGACCCTCTCTGTATGATCACTGAATACATGGAGAATGGA GATCTCAATCAGTTTCTTTCCCGCCACGAGCCCCCTAATTCTTCCTCCAGCGATGTACGC ACTGTCAGTTACACCAATCTGAAGTTTATGGCTACCCAAATTGCCTCTGGCATGAAGTAC CTTTCCTCTCTTAATTTTGTTCACCGAGATCTGGCCACACGAAACTGTTTAGTGGGTAAG AACTACACAATCAAGATAGCTGACTTTGGAATGAGCAGGAACCTGTACAGTGGTGACTAT TACCGGATCCAGGGCCGGGCAGTGCTCCCTATCCGCTGGATGTCTTGGGAGAGTATCTTG CTGGGCAAGTTCACTACAGCAAGTGATGTGTGGGCCTTTGGGGTTACTTTGTGGGAGACT TTCACCTTTTGTCAAGAACAGCCCTATTCCCAGCTGTCAGATGAACAGGTTATTGAGAAT ACTGGAGAGTTCTTCCGAGACCAAGGGAGGCAGACTTACCTCCCTCAACCAGCCATTTGT CCTGACTCTGTGTATAAGCTGATGCTCAGCTGCTGGAGAAGAGATACGAAGAACCGTCCC TCATTCCAAGAAATCCACCTTCTGCTCCTTCAACAAGGCGACGAGTGA Clone variation with respect to NM_006182.2 1260 c=>g;1823 a=>t;1824 a=>g >OriGene 5' read for mutant NM_006182 unedited
CCCGCCGTCTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTG ACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGTTTCACAACAAA TTCTTCTTTTTGGGTTGGGGAAACGCAGTGGATTATAGCTCTGTTTTCTTCTTTCCAAAACTGTGCACCC CTGGATGAAACCTCCATCAAGGGAGACCTACAAGTTGCCTGGGGTTCAGTGCTCTAGAAAGTTCCAAGGT TTGTGGCTTGAATTATTCTAAAGAAGCTGAAATAATTGAAGAGAAGCAGAGGCCAGCTGTTTTTTGAGGG ATCCTGCTCCACAGAGAAATGCTTCTGCCACCCCGTTGATACTTCCATTTCCAACACCCTTCTTTCTGAA ATAATCCTGATTCCCAGAAGGCTCTGGGGGCTGTTCCGGCTGCTGCCTATCTTGAGTTCTGCAAAGCCTC AGGTAATTCCGGCTTTATGCCCCTATCCTTGGGGCATGCAGGGAGGCAGAAATTCCGAAATAGGAACCCT CACAGTCTCCCAGTCAGTGGTCAAGAGTCCAGCTGTGCCATATGGGAAGGGCGGGACTCAAAAAAAGGGA TAGAGACCGGTGGCCTGAGATATCAGTGGGACCCGTGATGACTGTGAGAGATTTTTCACATTGTCTTGTG CACACTCCACATAAACTCTGGTGGGGGAGACACAGGCGCCCTCCGCGAGGTGACGGGTCTAGTTTTCCCC TGTGTACGATAATCTATCGCCGGATAGCGCACCTGGTGATCTTGCGGAAACGCATAGGGAACAAGTGCTG GATGAGATGTATACCTATGACATTTTCTAAGACTGTGAGACGCCATTGACAATGTTCGGCATCTGCACCG CCAACCTGATGTGGTCAATGAGCTACGCGTCGCGAAAGGCTGTTCAAGGTCAACTGAGCAAGAG >SC323423 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TKAATGCAGGTGGAATTCAAGACAAAGATTTTGCCCTAGATGTCAGTGCCAACCAGCCTGTCCTGGTGGC TGTGATGATGCTCCGAGCAGATGCCAACAAGAATGCCAGGAATGATTTTCTTAAGGAGATAAAGATCATG TCTCGGCTCAAGGACCCAAACATCATCCATCTATTAGCTGTGTGTATCACTGATGACCCTCTCTGTATGA TCACTGAATACATGGAGAATGGAGATCTCAATCAKTTTCTTTCCC |
Restriction Sites | Please inquire |
ACCN | NM_006182 |
Insert Size | 3100 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006182.2, NP_006173.2 |
RefSeq Size | 3172 bp |
RefSeq ORF | 2568 bp |
Locus ID | 4921 |
Cytogenetics | 1q23.3 |
Domains | F5_F8_type_C, pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
Gene Summary | 'This gene encodes a member of the discoidin domain receptor subclass of the receptor tyrosine kinase (RTKs) protein family. RTKs play a key role in the communication of cells with their microenvironment. The encoded protein is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. This protein is expressed in numerous cell types and may alos be involved in wound repair and regulate tumor growth and invasiveness. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia. [provided by RefSeq, Aug 2017]' Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116294 | DDR2 (untagged)-Human discoidin domain receptor tyrosine kinase 2 (DDR2), transcript variant 2 |
USD 310.00 |
|
RC211389 | DDR2 (Myc-DDK-tagged)-Human discoidin domain receptor tyrosine kinase 2 (DDR2), transcript variant 2 |
USD 750.00 |
|
RG211389 | DDR2 (GFP-tagged) - Human discoidin domain receptor tyrosine kinase 2 (DDR2), transcript variant 2 |
USD 830.00 |
|
RC211389L1 | Lenti ORF clone of Human discoidin domain receptor tyrosine kinase 2 (DDR2), transcript variant 2, Myc-DDK-tagged |
USD 1,176.00 |
|
RC211389L2 | Lenti ORF clone of Human discoidin domain receptor tyrosine kinase 2 (DDR2), transcript variant 2, mGFP tagged |
USD 950.00 |
|
RC211389L3 | Lenti ORF clone of Human discoidin domain receptor tyrosine kinase 2 (DDR2), transcript variant 2, Myc-DDK-tagged |
USD 950.00 |
|
RC211389L4 | Lenti ORF clone of Human discoidin domain receptor tyrosine kinase 2 (DDR2), transcript variant 2, mGFP tagged |
USD 950.00 |
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