CDKL5 (NM_003159) Human Untagged Clone

CAT#: SC323424

CDKL5 (untagged)-Kinase deficient mutant (K42M) of Human cyclin-dependent kinase-like 5 (CDKL5), transcript variant I

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDKL5
• Synonyms: CFAP247; EIEE2; ISSX; STK9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003159, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGATTCCTAACATTGGTAATGTGATGAATAAATTTGAGATCCTTGGGGTTGTAGGTGAAGGAGCCT
  ATGGAGTTGTACTTAAATGCAGACACAAGGAAACACATGAAATTGTGGCGATCAAGAAATTCAAGGACAG
  TGAAGAAAATGAAGAAGTCAAAGAAACGACTTTACGAGAGCTTAAAATGCTTCGGACTCTCAAGCAGGAA
  AACATTGTGGAGTTGAAGGAAGCATTTCGTCGGAGGGGAAAGTTGTACTTGGTGTTTGAGTATGTTGAAA
  AAAATATGCTCGAATTGCTGGAAGAAATGCCAAATGGAGTTCCACCTGAGAAAGTAAAAAGCTACATCTA
  TCAGCTAATCAAGGCTATTCACTGGTGCCATAAGAATGATATTGTCCATCGAGATATAAAACCAGAAAAT
  CTCTTAATCAGCCACAATGATGTCCTAAAACTGTGTGACTTTGGTTTTGCTCGTAATCTGTCAGAAGGCA
  ATAATGCTAATTACACAGAGTACGTTGCCACCAGATGGTATCGGTCCCCAGAACTCTTACTTGGCGCTCC
  CTATGGAAAGTCCGTGGACATGTGGTCGGTGGGCTGTATTCTTGGGGAGCTTAGCGATGGACAGCCTTTA
  TTTCCTGGAGAAAGTGAAATTGACCAACTTTTTACTATTCAGAAGGTGCTAGGACCACTTCCATCTGAGC
  AGATGAAGCTTTTCTACAGTAATCCTCGCTTCCATGGGCTCCGGTTTCCAGCTGTTAACCATCCTCAGTC
  CTTGGAAAGAAGATACCTTGGAATTTTGAATAGTGTTCTACTTGACCTAATGAAGAATTTACTGAAGTTG
  GACCCAGCTGACAGATACTTGACAGAACAGTGTTTGAATCACCCTACATTTCAAACCCAGAGACTTCTGG
  ATCGTTCTCCTTCAAGGTCAGCAAAAAGAAAACCTTACCATGTGGAAAGCAGCACATTGTCTAATAGAAA
  CCAAGCCGGCAAAAGTACTGCTTTGCAGTCTCACCACAGATCTAACAGCAAGGACATCCAGAACCTGAGT
  GTAGGCCTGCCCCGGGCTGACGAAGGTCTCCCTGCCAATGAAAGCTTCCTAAATGGAAACCTTGCTGGAG
  CTAGTCTTAGTCCACTGCACACCAAAACCTACCAAGCAAGCAGCCAGCCTGGGTCTACCAGCAAAGATCT
  CACCAACAACAACATACCACACCTTCTTAGCCCAAAAGAAGCCAAGTCAAAAACAGAGTTTGATTTTAAT
  ATTGACCCAAAGCCTTCAGAAGGCCCAGGGACAAAGTACCTCAAGTCAAACAGCAGATCTCAGCAGAACC
  GCCACTCATTCATGGAAAGCTCTCAAAGCAAAGCTGGGACACTGCAGCCCAATGAAAAGCAGAGTCGGCA
  TAGCTATATTGACACAATTCCCCAGTCCTCTAGGAGTCCCTCCTACAGGACCAAGGCCAAAAGCCATGGG
  GCACTGAGTGACTCCAAGTCTGTGAGCAACCTTTCTGAAGCCAGGGCCCAAATTGCGGAGCCCAGTACCA
  GTAGGTACTTCCCATCTAGCTGCTTAGACTTGAATTCTCCCACCAGCCCAACCCCCACCAGACACAGTGA
  CACGAGAACTTTGCTCAGCCCTTCTGGAAGAAATAACCGAAATGAGGGAACGCTGGACTCACGTCGAACC
  ACAACCAGACATTCTAAGACGATGGAGGAATTGAAGCTGCCGGAGCACATGGACAGTAGCCATTCCCATT
  CACTGTCTGCACCTCACGAATCTTTTTCTTATGGACTGGGCTACACCAGCCCCTTTTCTTCCCAGCAACG
  TCCTCATAGGCATTCTATGTATGTGACCCGTGACAAAGTGAGAGCCAAGGGCTTGGATGGAAGCTTGAGC
  ATAGGGCAAGGGATGGCAGCTAGAGCCAACAGCCTGCAACTCTTGTCACCCCAGCCTGGAGAACAGCTCC
  CTCCAGAGATGACTGTGGCAAGATCTTCGGTCAAAGAGACCTCCAGAGAAGGCACCTCTTCCTTCCATAC
  ACGCCAGAAGTCTGAGGGTGGAGTGTATCATGACCCACACTCTGATGATGGCACAGCCCCCAAAGAAAAT
  AGACACCTATACAATGATCCTGTGCCAAGGAGAGTTGGTAGCTTTTACAGAGTGCCATCTCCACGTCCAG
  ACAATTCTTTCCATGAAAATAATGTGTCAACTAGAGTTTCTTCTCTACCATCAGAGAGCAGTTCTGGAAC
  CAACCACTCAAAAAGACAACCAGCATTCGATCCATGGAAAAGTCCTGAAAATATTAGTCATTCAGAGCAA
  CTCAAGGAAAAAGAGAAGCAAGGATTTTTCAGGTCAATGAAAAAGAAAAAGAAGAAATCTCAAACAGTAC
  CCAATTCCGACAGCCCTGATCTTCTGACGTTGCAGAAATCCATTCATTCTGCTAGCACTCCAAGCAGCAG
  ACCAAAGGAGTGGCGCCCCGAGAAGATCTCAGATCTGCAGACCCAAAGCCAGCCATTAAAATCACTGCGC
  AAGTTGTTACATCTCTCTTCGGCCTCAAATCACCCGGCTTCCTCAGATCCCCGCTTCCAGCCCTTAACAG
  CTCAACAAACCAAAAATTCCTTCTCAGAAATTCGGATTCACCCCCTGAGCCAGGCCTCTGGCGGGAGCAG
  CAACATCCGGCAGGAACCCGCACCGAAGGGCAGGCCAGCCCTCCAGCTGCCAGACGGTGGATGTGATGGC
  AGAAGACAGAGACACCATTCTGGACCCCAAGATAGACGCTTCATGTTAAGGACGACAGAACAACAAGGAG
  AATACTTCTGCTGTGGTGACCCAAAGAAGCCTCACACTCCGTGCGTCCCAAACCGAGCCCTTCATCGTCC
  AATCTCCAGTCCTGCTCCCTATCCAGTACTCCAGGTCCGAGGCACTTCCATGTGCCCGACACTCCAGGTC
  CGAGGCACTGATGCTTTCAGCTGCCCAACCCAGCAATCCGGGTTCTCTTTCTTCGTGAGACACGTTATGA
  GGGAAGCCCTGATTCACAGGGCCCAGGTAAACCAAGCTGCGCTCCTGACATACCATGAGAATGCGGCACT
  GACGGGCAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003159 unedited
  CCCGCCCGTATCAGCAATTGGGCGGTAGGCGCTGTACGGTGGGAGGCTCTATATAAGCAGAGCTCGTTTA
  GTGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGACGG
  CGTCCTCAGGAGCTGTGGGGTCCCCTGCTAGAAGTGGGGGACTCGGCGGGGGAGTCATTTAATACTTCAT
  GATTAGAACAAATATGTGAAAGTTCCCACCAACCAGTGAGAATTTCTTCCTTCAGACGGTTTTGGATCTT
  ACTGCACAGCTTTCTGAGAAGTTCTTTTGGTGCCATGTTTTGTGGGCTTGCATCAAAAGAAGGAGTTTGT
  CTTTCATGAAGAATTCCTAAACAATTGGTAAATGTGGATTGAAATAAAATTTGAGAATCCTTGGGGGTGT
  GTAGGTGGAAGAGACCCCTATGGGATTTGGTACTTAATGGGCAAACCCCAAGGGAACCAACATGGAAATT
  GGGGGCAAATCATGAAAATTCAGGGCCGTGTGAAAAAAAATGAAAAAGTTCAAAAAAACAAACTTTCCAA
  AGACCTAAAATGGCTTCGGAACTCTAACCCGGAAAAACCTTTGGGGGATTAAAAGGAACCCTTTTCCCCG
  AGGGGAAAATTTTCCCTTGGGGTTTGAGAATTTTTAAAAAAAAATTCCCCAAATTTGGGGGAAAAATGCC
  CAAGGGGTTTCCCCCCGGAAAAATTAAACGCCACTTTTTTCCTATATCGGGCGTTTTCTGGTGGCGGTAA
  AAAAATATTTTCCCCCACAAAAATAAACCCAAAAATCCTATAACCCCCCAGAAGGGCCCAAACTGTGAGT
  TTTGGTTTTTCGGATATCTCGAAAGACGCATAGCCGTTTTTACCACACGTGTCCCCCACATGGGGTGTTG
  CCCCCAAACATCTTATGGCGCCTCTCAGAAAAACCCTGCAGACTGAGTCGGAGCCGCGTTCTGGGGCGTA
  CACGCGCGAACATTTCTTCTGAGCAGAGAAGTAGACCCTCTCGTTATATAGAG
  
  Kinase domain sequence

  >SC323424 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTCKMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG
  AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGACGGCGTCCTCAGGAG
  CTGTGGGGTCCCCTGCTAGAAGTGGGGGACTCGGCGGGGGAGTCATTTAATACTTCATGATTAGAACAAA
  TATGTGAAAGTTCCCACCAACCAGTGAGAATTTCTTCCTTCAGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_003159
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003159.1, NP_003150.1
• RefSeq Size: 3434 bp
• RefSeq ORF: 3093 bp
• Locus ID: 6792
• Cytogenetics: Xp22.13
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene is a member of Ser/Thr protein kinase family and encodes a phosphorylated protein with protein kinase activity. Mutations in this gene have been associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (I) represents the longer transcript. Variants I and II encode the same protein.