PKR (EIF2AK2) (NM_002759) Human Untagged Clone

CAT#: SC323431

EIF2AK2 (untagged)-Kinase deficient mutant (K296M) of Human eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2), transcript variant 1


  "NM_002759" in other vectors (7)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol EIF2AK2
Synonyms EIF2AK1; PKR; PPP1R83; PRKR
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323431 sequence for NM_002759 edited (data generated by NextGen Sequencing)
ATGGCTGGTGATCTTTCAGCAGGTTTCTTCATGGAGGAACTTAATACATACCGTCAGAAG
CAGGGAGTAGTACTTAAATATCAAGAACTGCCTAATTCAGGACCTCCACATGATAGGAGG
TTTACATTTCAAGTTATAATAGATGGAAGAGAATTTCCAGAAGGTGAAGGTAGATCAAAG
AAGGAAGCAAAAAATGCCGCAGCCAAATTAGCTGTTGAGATACTTAATAAGGAAAAGAAG
GCAGTTAGTCCTTTATTATTGACAACAACGAATTCTTCAGAAGGATTATCCATGGGGAAT
TACATAGGCCTTATCAATAGAATTGCCCAGAAGAAAAGACTAACTGTAAATTATGAACAG
TGTGCATCGGGGGTGCATGGGCCAGAAGGATTTCATTATAAATGCAAAATGGGACAGAAA
GAATATAGTATTGGTACAGGTTCTACTAAACAGGAAGCAAAACAATTGGCCGCTAAACTT
GCATATCTTCAGATATTATCAGAAGAAACCTCAGTGAAATCTGACTACCTGTCCTCTGGT
TCTTTTGCTACTACGTGTGAGTCCCAAAGCAACTCTTTAGTGACCAGCACACTCGCTTCT
GAATCATCATCTGAAGGTGACTTCTCAGCAGATACATCAGAGATAAATTCTAACAGTGAC
AGTTTAAACAGTTCTTCGTTGCTTATGAATGGTCTCAGAAATAATCAAAGGAAGGCAAAA
AGATCTTTGGCACCCAGATTTGACCTTCCTGACATGAAAGAAACAAAGTATACTGTGGAC
AAGAGGTTTGGCATGGATTTTAAAGAAATAGAATTAATTGGCTCAGGTGGATTTGGCCAA
GTTTTCAAAGCAAAACACAGAATTGACGGAAAGACTTACGTTATTATGCGTGTTAAATAT
AATAACGAGAAGGCGGAGCGTGAAGTAAAAGCATTGGCAAAACTTGATCATGTAAATATT
GTTCACTACAATGGCTGTTGGGATGGATTTGATTATGATCCTGAGACCAGTGATGATTCT
CTTGAGAGCAGTGATTATGATCCTGAGAACAGCAAAAATAGTTCAAGGTCAAAGACTAAG
TGCCTTTTCATCCAAATGGAATTCTGTGATAAAGGGACCTTGGAACAATGGATTGAAAAA
AGAAGAGGCGAGAAACTAGACAAAGTTTTGGCTTTGGAACTCTTTGAACAAATAACAAAA
GGGGTGGATTATATACATTCAAAAAAATTAATTCATAGAGATCTTAAGCCAAGTAATATA
TTCTTAGTAGATACAAAACAAGTAAAGATTGGAGACTTTGGACTTGTAACATCTCTGAAA
AATGATGGAAAGCGAACAAGGAGTAAGGGAACTTTGCGATACATGAGCCCAGAACAGATT
TCTTCGCAAGACTATGGAAAGGAAGTGGACCTCTACGCTTTGGGGCTAATTCTTGCTGAA
CTTCTTCATGTATGTGACACTGCTTTTGAAACATCAAAGTTTTTCACAGACCTACGGGAT
GGCATCATCTCAGATATATTTGATAAAAAAGAAAAAACTCTTCTACAGAAATTACTCTCA
AAGAAACCTGAGGATCGACCTAACACATCTGAAATACTAAGGACCTTGACTGTGTGGAAG
AAAAGCCCAGAGAAAAATGAACGACACACATGTTAG

Clone variation with respect to NM_002759.2
887 a=>t;888 a=>g
>OriGene 5' read for mutant NM_002759 unedited
ACCGCCGTCTCAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGT
GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCCGGCGGCG
GCGGCGGCGGCGGCGGCGGCGCAGTTTCTGGAGCAAATTCAGTTTGCCTTCCTGGATTTGTAAATCGTAA
TGACCTCAAAACTTTAGCAGTTCTTCCATCTGACTCAGGTTTGCTTCTCTGGCGGTCTTCAGAATCAACA
TCCACACTTTCCGTGATTATCTGCGTGCATTTTTGGACAAAGCTTCCAACCAGGATACGGGAAAGAAAGA
AAATGGCTGGTTGATCTTTCAGCAGGTTTTCTTTCATGGGAGGACTTTAATACCATACCCGTCAGAGGCA
GGGAGTAGTACCTAAATTATCAGAACTGCCCTATTTCAGGACCTCCCCATGAATAGGAGGTTACCTTTTC
AAGTTTTAATAGATGGAAAAAATTTCCAGGAGGTGAGGTAGATCAAAGGAGGGAGCAAAAAGGCCGACCC
AATTGCTGTTGAAACCTAAATAGAAAGAAAGCGAGTATCCTTATATATGGCACACACAATCTTCAAGGTA
TCCTGGGGATTCCAGCCTTCAATAATTGGCCAAGAAGACTACGTGATATTGACATGGCTCTCGGTCATGC
CCAGATTCTTTAATGCCAATGGCGAGAAATGTTGTCAGTTCCTAAACGAGACACACTGGCGTACTCGATC
CGATTACAGGACCTTGAATCGACTGCCGGTTGGCACTTGAATCAAACTTTAGACGACTCTGACTCAGATC
CGATCGATATT
>SC323431 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TWTGATTATTGGCTCAGKGGWTTGGCCAAGTTTTCAAGCAAAACACAGAATTGACGGAAAGACTTACGTT
ATTATGCGTGTTAAATATAATAACGAGAAGGCGGAGCGTGAAGTAAAAGCATTGGCAAAACTTGATCATG
TAAATATTGTTCACTACAATGGCTGTTGGGATGGATTTGATTATGATCCTGAGACCAGTGATGATTCTCT
TGAGAGCAGTGATTATGATCCTGAGAACAGCAAAAATAGTTCAAG
Restriction Sites Please inquire     
ACCN NM_002759
Insert Size 2580 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002759.1, NP_002750.1
RefSeq Size 2808 bp
RefSeq ORF 1656 bp
Locus ID 5610
Cytogenetics 2p22.2
Domains pkinase, DSRM, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase, Transcription Factors
Gene Summary 'The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 both encode the same protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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