NEK8 (NM_178170) Human Untagged Clone
CAT#: SC323448
NEK8 (untagged)-Kinase deficient mutant (K33M) of Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8)
"NM_178170" in other vectors (7)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | NEK8 |
Synonyms | JCK; NEK12A; NPHP9; RHPD2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_178170, the custom clone sequence may differ by one or more nucleotides
ATGGAGAAGTACGAGCGGATCCGAGTGGTGGGGAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTGCGAA AGGCTGACCAGAAGCTGGTGATCATCAAGCAGATTCCAGTGGAACAGATGACCAAGGAAGAGCGGCAGGC AGCCCAGAATGAGTGCCAGGTCCTCAAGCTGCTCAACCACCCCAATGTCATTGAGTACTACGAGAACTTC CTGGAAGACAAAGCCCTTATGATCGCCATGGAATATGCACCAGGCGGCACTCTGGCTGAGTTCATCCAAA AGCGCTGTAATTCCCTGCTGGAGGAGGAGACCATCCTGCACTTCTTCGTGCAGATCCTGCTTGCACTGCA TCATGTGCACACCCACCTCATCCTGCACCGAGACCTCAAGACCCAGAACATCCTGCTTGACAAACACCGC ATGGTCGTCAAGATCGGTGATTTCGGCATCTCCAAGATCCTTAGCAGCAAGAGCAAGGCCTACACGGTGG TGGGTACCCCATGCTATATCTCCCCTGAGCTGTGTGAGGGCAAGCCCTACAACCAGAAGAGTGACATCTG GGCCCTGGGCTGTGTCCTCTACGAGCTGGCCAGCCTCAAGAGGGCTTTCGAGGCTGCGAACTTGCCAGCA CTGGTGCTGAAGATCATGAGTGGCACCTTTGCACCTATCTCTGACCGGTACAGCCCTGAGCTTCGCCAGC TGGTCCTGAGTCTACTCAGCCTGGAGCCTGCCCAGCGGCCACCACTCAGCCACATCATGGCACAGCCCCT CTGCATCCGTGCCCTCCTCAACCTCCACACCGACGTGGGCAGTGTCCGCATGCGGAGGGCAGAGAAGTCC GTGGCCCCCAGCAACACAGGGAGCAGGACCACCAGTGTCCGCTGCAGAGGTATCCCCCGGGGACCTGTGA GGCCAGCCATCCCACCACCACTGTCGTCAGTGTATGCCTGGGGTGGTGGGCTGGGCACCCCCCTGCGGCT GCCAATGCTCAACACAGAGGTGGTCCAGGTGGCAGCTGGGCGCACGCAGAAAGCCGGCGTCACGCGCTCT GGGCGTCTCATCCTGTGGGAGGCCCCACCCCTAGGTGCAGGCGGAGGCAGTCTCCTTCCTGGGGCAGTGG AGCAGCCACAGCCCCAGTTCATCTCGCGTTTCCTGGAGGGCCAGTCGGGTGTGACCATCAAGCACGTGGC CTGTGGGGACTTCTTCACTGCCTGCCTGACTGACAGAGGCATCATCATGACATTCGGCAGCGGCAGCAAT GGGTGCCTAGGCCATGGCAGCCTCACTGACATCAGCCAGCCCACCATTGTGGAGGCTTTGCTGGGCTATG AAATGGTGCAGGTGGCCTGTGGGGCCTCTCACGTGCTGGCCCTGTCCACTGAGCGAGAACTATTTGCCTG GGGCCGTGGAGACAGCGGCAGACTGGGGCTAGGCACCAGGGAGTCCCACAGCTGCCCCCAGCAGGTGCCC ATGCCCCCAGGACAGGAAGCTCAGCGAGTTGTATGTGGTATCGATTCCTCCATGATCCTCACTGTGCCTG GCCAAGCCCTAGCCTGTGGGAGCAACAGGTTCAACAAGCTGGGCCTGGACCACCTCTCCCTGGGGGAGGA GCCTGTCCCCCACCAGCAAGTGGAGGAGGCCCTGAGCTTCACACTACTAGGCTCTGCACCCCTGGACCAG GAGCCTCTGCTGAGTATAGACCTGGGCACTGCTCACTCAGCTGCTGTGACTGCCTCGGGTGATTGCTACA CTTTTGGCAGCAATCAGCACGGACAGTTGGGCACCAATACTCGCCGAGGCAGTCGGGCACCCTGTAAGGT CCAAGGCCTTGAGGGCATCAAGATGGCAATGGTAGCCTGTGGGGATGCCTTCACTGTAGCTATTGGGGCA GAGAGCGAAGTGTACTCTTGGGGCAAAGGGGCGCGAGGTCGATTGGGAAGGAGGGATGAGGATGCCGGAC TCCCTCGGCCAGTGCAGTTGGATGAGACACACCCTTACACGGTGACTTCCGTGTCCTGTTGCCATGGAAA CACCCTCCTGGCTGTTCGATCGGTCACAGATGAGCCGGTCCCCCCCTGA >OriGene 5' read for mutant NM_178170 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGAGAGAGAAGT ACGAGCGGATCCGAGTGGTGGGGAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTGCGAAAGGCTGACCA GAAGCTGGTGATCATCATGCAGATTCCAGTGGAACAGATGACCAAGGAAGAGCGGCAGGCAGCCCAGAAT GAGTGCCAGGTCCTCAAGCTGCTCAACCACCCCAATGTCATTTGAGTACTACGAGAACTTTCCTGGAAGA CAAAGCCCCTTTATGATCGGCCATGGGAAATATGGCACCAGGCGGGCACTCTGGCTGAGTTCATCCCAAA AAGCCGCTGGTATTTCCCTGGCTTGGAAGGAAGAAGACCATTCCTGCAATTTCTTTCGTGCGAATCCCTG GCTTGCACTGCCATCATGGTGCACACCCACTTTAATCCTTGCACGAAAACCTAAAAACCCAAAACATCTG TGTTTGACAAACCCCGCAGGGCCTTAAAGATCGGGAATTTGCGGCATCTCCGAATATCTTAACAGCAAAG CCAAGGGCCTACAGGGGGGGTGGGATCCCATCTCTATTTTCCCGAGATCTGTGAGAGGACACCCTCTACA CCAAGAGAAGAACTGGGGCCCGGGGTGTGTCTCTAGATGTGGCCCCTAGGGTTTAGACGCGACAATTCGC ATCGTGCGCAGATATAGATGGGCCATTGTCCCTATGTCCGCCGAACCGAGTGTCCCTGTGTCAGTATCAT CAGATACGACAGGCCCATCAACAATAGAGAACACTGTACTGTTGGCTCTTCATTTACACCATTGTAGATG TACGTGTGAGAGA >SC323448 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYCAGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGAGAGAGAAGTACGAGC GGATCCGAGTGGTGGGGAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTGCGAAAGGCTGACCAGAAGCT GGTGATCATCATGCAGATTCCAGTGGAACAGATGACCAAGGAAGA |
Restriction Sites | Please inquire |
ACCN | NM_178170 |
ORF Size | 2079 bp |
Insert Size | 2700 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Reference Data | |
RefSeq | NM_178170.2, NP_835464.1 |
RefSeq Size | 2858 |
RefSeq ORF | 2079 |
Locus ID | 284086 |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC127876 | NEK8 (untagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
USD 1,410.00 |
|
RC216021 | NEK8 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
USD 770.00 |
|
RG216021 | NEK8 (GFP-tagged) - Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
USD 850.00 |
|
RC216021L1 | Lenti-ORF clone of NEK8 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
USD 1,200.00 |
|
RC216021L2 | Lenti-ORF clone of NEK8 (mGFP-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
USD 970.00 |
|
RC216021L3 | Lenti-ORF clone of NEK8 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
USD 970.00 |
|
RC216021L4 | Lenti-ORF clone of NEK8 (mGFP-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
USD 970.00 |
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