NEK8 (NM_178170) Human Untagged Clone

CAT#: SC323448

NEK8 (untagged)-Kinase deficient mutant (K33M) of Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEK8
• Synonyms: JCK; NEK12A; NPHP9; RHPD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_178170, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGAAGTACGAGCGGATCCGAGTGGTGGGGAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTGCGAA
  AGGCTGACCAGAAGCTGGTGATCATCAAGCAGATTCCAGTGGAACAGATGACCAAGGAAGAGCGGCAGGC
  AGCCCAGAATGAGTGCCAGGTCCTCAAGCTGCTCAACCACCCCAATGTCATTGAGTACTACGAGAACTTC
  CTGGAAGACAAAGCCCTTATGATCGCCATGGAATATGCACCAGGCGGCACTCTGGCTGAGTTCATCCAAA
  AGCGCTGTAATTCCCTGCTGGAGGAGGAGACCATCCTGCACTTCTTCGTGCAGATCCTGCTTGCACTGCA
  TCATGTGCACACCCACCTCATCCTGCACCGAGACCTCAAGACCCAGAACATCCTGCTTGACAAACACCGC
  ATGGTCGTCAAGATCGGTGATTTCGGCATCTCCAAGATCCTTAGCAGCAAGAGCAAGGCCTACACGGTGG
  TGGGTACCCCATGCTATATCTCCCCTGAGCTGTGTGAGGGCAAGCCCTACAACCAGAAGAGTGACATCTG
  GGCCCTGGGCTGTGTCCTCTACGAGCTGGCCAGCCTCAAGAGGGCTTTCGAGGCTGCGAACTTGCCAGCA
  CTGGTGCTGAAGATCATGAGTGGCACCTTTGCACCTATCTCTGACCGGTACAGCCCTGAGCTTCGCCAGC
  TGGTCCTGAGTCTACTCAGCCTGGAGCCTGCCCAGCGGCCACCACTCAGCCACATCATGGCACAGCCCCT
  CTGCATCCGTGCCCTCCTCAACCTCCACACCGACGTGGGCAGTGTCCGCATGCGGAGGGCAGAGAAGTCC
  GTGGCCCCCAGCAACACAGGGAGCAGGACCACCAGTGTCCGCTGCAGAGGTATCCCCCGGGGACCTGTGA
  GGCCAGCCATCCCACCACCACTGTCGTCAGTGTATGCCTGGGGTGGTGGGCTGGGCACCCCCCTGCGGCT
  GCCAATGCTCAACACAGAGGTGGTCCAGGTGGCAGCTGGGCGCACGCAGAAAGCCGGCGTCACGCGCTCT
  GGGCGTCTCATCCTGTGGGAGGCCCCACCCCTAGGTGCAGGCGGAGGCAGTCTCCTTCCTGGGGCAGTGG
  AGCAGCCACAGCCCCAGTTCATCTCGCGTTTCCTGGAGGGCCAGTCGGGTGTGACCATCAAGCACGTGGC
  CTGTGGGGACTTCTTCACTGCCTGCCTGACTGACAGAGGCATCATCATGACATTCGGCAGCGGCAGCAAT
  GGGTGCCTAGGCCATGGCAGCCTCACTGACATCAGCCAGCCCACCATTGTGGAGGCTTTGCTGGGCTATG
  AAATGGTGCAGGTGGCCTGTGGGGCCTCTCACGTGCTGGCCCTGTCCACTGAGCGAGAACTATTTGCCTG
  GGGCCGTGGAGACAGCGGCAGACTGGGGCTAGGCACCAGGGAGTCCCACAGCTGCCCCCAGCAGGTGCCC
  ATGCCCCCAGGACAGGAAGCTCAGCGAGTTGTATGTGGTATCGATTCCTCCATGATCCTCACTGTGCCTG
  GCCAAGCCCTAGCCTGTGGGAGCAACAGGTTCAACAAGCTGGGCCTGGACCACCTCTCCCTGGGGGAGGA
  GCCTGTCCCCCACCAGCAAGTGGAGGAGGCCCTGAGCTTCACACTACTAGGCTCTGCACCCCTGGACCAG
  GAGCCTCTGCTGAGTATAGACCTGGGCACTGCTCACTCAGCTGCTGTGACTGCCTCGGGTGATTGCTACA
  CTTTTGGCAGCAATCAGCACGGACAGTTGGGCACCAATACTCGCCGAGGCAGTCGGGCACCCTGTAAGGT
  CCAAGGCCTTGAGGGCATCAAGATGGCAATGGTAGCCTGTGGGGATGCCTTCACTGTAGCTATTGGGGCA
  GAGAGCGAAGTGTACTCTTGGGGCAAAGGGGCGCGAGGTCGATTGGGAAGGAGGGATGAGGATGCCGGAC
  TCCCTCGGCCAGTGCAGTTGGATGAGACACACCCTTACACGGTGACTTCCGTGTCCTGTTGCCATGGAAA
  CACCCTCCTGGCTGTTCGATCGGTCACAGATGAGCCGGTCCCCCCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_178170 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGAGAGAGAAGT
  ACGAGCGGATCCGAGTGGTGGGGAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTGCGAAAGGCTGACCA
  GAAGCTGGTGATCATCATGCAGATTCCAGTGGAACAGATGACCAAGGAAGAGCGGCAGGCAGCCCAGAAT
  GAGTGCCAGGTCCTCAAGCTGCTCAACCACCCCAATGTCATTTGAGTACTACGAGAACTTTCCTGGAAGA
  CAAAGCCCCTTTATGATCGGCCATGGGAAATATGGCACCAGGCGGGCACTCTGGCTGAGTTCATCCCAAA
  AAGCCGCTGGTATTTCCCTGGCTTGGAAGGAAGAAGACCATTCCTGCAATTTCTTTCGTGCGAATCCCTG
  GCTTGCACTGCCATCATGGTGCACACCCACTTTAATCCTTGCACGAAAACCTAAAAACCCAAAACATCTG
  TGTTTGACAAACCCCGCAGGGCCTTAAAGATCGGGAATTTGCGGCATCTCCGAATATCTTAACAGCAAAG
  CCAAGGGCCTACAGGGGGGGTGGGATCCCATCTCTATTTTCCCGAGATCTGTGAGAGGACACCCTCTACA
  CCAAGAGAAGAACTGGGGCCCGGGGTGTGTCTCTAGATGTGGCCCCTAGGGTTTAGACGCGACAATTCGC
  ATCGTGCGCAGATATAGATGGGCCATTGTCCCTATGTCCGCCGAACCGAGTGTCCCTGTGTCAGTATCAT
  CAGATACGACAGGCCCATCAACAATAGAGAACACTGTACTGTTGGCTCTTCATTTACACCATTGTAGATG
  TACGTGTGAGAGA
  
  Kinase domain sequence

  >SC323448 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYCAGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGAGAGAGAAGTACGAGC
  GGATCCGAGTGGTGGGGAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTGCGAAAGGCTGACCAGAAGCT
  GGTGATCATCATGCAGATTCCAGTGGAACAGATGACCAAGGAAGA
  
• Restriction Sites: Please inquire
• ACCN: NM_178170
• ORF Size: 2079 bp
• Insert Size: 2700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_178170.2, NP_835464.1
• RefSeq Size: 2858
• RefSeq ORF: 2079
• Locus ID: 284086
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans. [provided by RefSeq, Jul 2008]