DAP Kinase 1 (DAPK1) (NM_004938) Human Untagged Clone

CAT#: SC323458

DAPK1 (untagged)-Kinase deficient mutant (K42M) of Human death-associated protein kinase 1 (DAPK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAPK1
• Synonyms: DAPK; ROCO3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323458 sequence for NM_004938 edited (data generated by NextGen Sequencing)
  ATGACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACACCGGCGAGGAACTTGGC
  AGTGGACAGTTTGCGGTTGTGAAGAAATGCCGTGAGAAAAGCACCGGCCTCCAGTATGCC
  GCCATGTTCATCAAGAAAAGGAGGACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGAC
  ATCGAGCGGGAGGTCAGCATCCTGAAGGAGATCCAGCACCCCAATGTCATCACCCTGCAC
  GAGGTCTATGAGAACAAGACGGACGTCATCCTGATCTTGGAACTCGTTGCAGGTGGCGAG
  CTGTTTGACTTCTTAGCTGAAAAGGAATCTTTAACTGAAGAGGAAGCAACTGAATTTCTC
  AAACAAATTCTTAATGGTGTTTACTACCTGCACTCCCTTCAAATCGCCCACTTTGATCTT
  AAGCCTGAGAACATAATGCTTTTGGATAGAAATGTCCCCAAACCTCGGATCAAGATCATT
  GACTTTGGGTTGGCCCATAAAATTGACTTTGGAAATGAATTTAAAAACATATTTGGGACT
  CCAGAGTTTGTCGCTCCTGAGATAGTCAACTATGAACCTCTTGGTCTTGAGGCAGATATG
  TGGAGTATCGGGGTAATAACCTATATCCTCCTAAGTGGGGCCTCCCCATTTCTTGGAGAC
  ACTAAGCAAGAAACGTTAGCAAATGTATCCGCTGTCAACTACGAATTTGAGGATGAATAC
  TTCAGTAATACCAGTGCCCTAGCCAAAGATTTCATAAGAAGACTTCTGGTCAAGGATCCA
  AAGAAGAGAATGACAATTCAAGATAGTTTGCAGCATCCCTGGATCAAGCCTAAAGATACA
  CAACAGGCACTTAGTAGAAAAGCATCAGCAGTAAACATGGAGAAATTCAAGAAGTTTGCA
  GCCCGGAAAAAATGGAAACAATCCGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGG
  TCATTCCTGTCCAGAAGTAACATGAGTGTTGCCAGAAGCGATGATACTCTGGATGAGGAA
  GACTCCTTTGTGATGAAAGCCATCATCCATGCCATCAACGATGACAATGTCCCAGGCCTG
  CAGCACCTTCTGGGCTCATTATCCAACTATGATGTTAACCAACCCAACAAGCACGGGACA
  CCTCCATTACTCATTGCTGCTGGCTGTGGGAATATTCAAATACTACAGTTGCTCATTAAA
  AGAGGCTCGAGAATCGATGTCCAGGATAAGGGCGGGTCCAATGCCGTCTACTGGGCTGCT
  CGGCATGGCCACGTCGATACCTTGAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTG
  AAAGACAAGTCTGGAGAGATGGCCCTCCACGTGGCAGCTCGCTATGGCCATGCTGACGTG
  GCTCAGTTACTGTGCAGCTTCGGCTCAAATCCCAATATCCAGGACAAGGAAGAAGAAACC
  CCCCTGCACTGTGCTGCTTGGCACGGCTATTACTCTGTGGCCAAAGCCCTTTGTGAAGCC
  GGCTGTAACGTGAACATCAAGAACCGAGAAGGAGAGACGCCCCTCCTGACAGCCTCTGCC
  AGGGGCTACCACGACATCGTGGAGTGTCTGGCCGAACATGGAGCCGATCTTAATGCTTGC
  GACAAGGACGGACACATTGCCCTTCATCTGGCTGTAAGACGGTGTCAGATGGAGGTAATC
  AAGACTCTCCTCAGCCAAGGGTGTTTCGTCGATTATCAAGACAGGCACGGCAATACTCCC
  CTCCATGTGGCATGTAAAGATGGCAACATGCCTATCGTGGTGGCCCTCTGTGAAGCAAAC
  TGCAATTTGGACATCTCCAACAAGTATGGGCGAACGCCTCTGCACCTTGCGGCCAACAAC
  GGAATCCTAGACGTGGTCCGGTATCTCTGTCTGATGGGAGCCAGCGTTGAGGCGCTGACC
  ACGGACGGAAAGACGGCAGAAGATCTTGCTAGATCGGAACAGCACGAGCACGTAGCAGGT
  CTCCTTGCAAGACTTCGAAAGGATACGCACCGAGGACTCTTCATCCAGCAGCTCCGACCC
  ACACAGAACCTGCAGCCAAGAATTAAGCTCAAGCTGTTTGGCCACTCGGGATCCGGGAAA
  ACCACCCTTGTAGAATCTCTCAAGTGTGGGCTGCTGAGGAGCTTTTTCAGAAGGCGTCGG
  CCCAGACTGTCTTCCACCAACTCCAGCAGGTTCCCACCTTCACCCCTGGCTTCTAAGCCC
  ACAGTCTCAGTGAGCATCAACAACCTGTACCCAGGCTGCGAGAACGTGAGTGTGAGGAGC
  CGCAGCATGATGTTCGAGCCGGGTCTTACCAAAGGGATGCTGGAGGTGTTTGTGGCCCCG
  ACCCACCACCCGCACTGCTCGGCCGATGACCAGTCCACCAAGGCCATCGACATCCAGAAC
  GCTTATTTGAATGGAGTTGGCGATTTCAGCGTGTGGGAGTTCTCTGGAAATCCTGTGTAT
  TTCTGCTGTTATGACTATTTTGCTGCAAATGATCCCACGTCAATCCATGTTGTTGTCTTT
  AGTCTAGAAGAGCCCTATGAGATCCAGCTGAACCAAGTGATTTTCTGGCTCAGTTTCCTG
  AAGTCCCTTGTCCCAGTTGAAGAACCCATAGCCTTCGGTGGCAAGCTGAAGAACCCACTC
  CAAGTTGTCCTGGTGGCCACCCACGCTGACATCATGAATGTTCCTCGACCGGCTGGAGGC
  GAGTTTGGATATGACAAAGACACATCGTTGCTGAAAGAGATTAGGAACAGGTTTGGAAAT
  GATCTTCACATTTCAAATAAGCTGTTTGTTCTGGATGCTGGGGCTTCTGGGTCAAAGGAC
  ATGAAGGTACTTCGAAATCATCTGCAAGAAATACGAAGCCAGATTGTTTCGGTCTGTCCT
  CCCATGACTCACCTGTGTGAGAAAATCATCTCCACGCTGCCTTCCTGGAGGAAGCTCAAT
  GGACCCAACCAGCTGATGTCGCTGCAGCAGTTTGTGTACGACGTGCAGGACCAGCTGAAC
  CCCCTGGCCAGCGAGGAGGACCTCAGGCGCATTGCTCAGCAGCTCCACAGCACAGGCGAG
  ATCAACATCATGCAAAGTGAAACAGTTCAGGACGTGCTGCTCCTGGACCCCCGCTGGCTC
  TGCACAAACGTCCTGGGGAAGTTGCTGTCCGTGGAGACCCCACGGGCGCTGCACCACTAC
  CGGGGCCGCTACACCGTGGAGGACATCCAGCGCCTGGTGCCCGACAGCGACGTGGAGGAG
  CTGCTGCAGATCCTCGATGCCATGGACATCTGCGCCCGGGACCTGAGCAGCGGGACCATG
  GTGGACGTCCCAGCCCTGATCAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAG
  GACGAGGTGATGGTGTATGGTGGCGTGCGCATCGTGCCCGTGGAACACCTCACCCCCTTC
  CCATGTGGCATCTTTCACAAGGTCCAGGTGAACCTGTGCCGGTGGATCCACCAGCAAAGC
  ACAGAGGGCGACGCGGACATCCGCCTGTGGGTGAATGGCTGCAAGCTGGCCAACCGTGGG
  GCCGAGCTGCTGGTGCTGCTGGTCAACCACGGCCAGGGCATTGAGGTCCAGGTCCGTGGC
  CTGGAGACGGAGAAGATCAAGTGCTGCCTGCTGCTGGACTCGGTGTGCAGCACCATTGAG
  AACGTCATGGCCACCACGCTGCCAGGGCTCCTGACCGTGAAGCATTACCTGAGCCCCCAG
  CAGCTGCGGGAGCACCATGAGCCCGTCATGATCTACCAGCCACGGGACTTCTTCCGGGCA
  CAGACTCTGAAGGAAACCTCACTGACCAACACCATGGGGGGGTACAAGGAAAGCTTCAGC
  AGCATCATGTGCTTCGGGTGTCACGACGTCTACTCACAGGCCAGCCTCGGCATGGACATC
  CATGCATCAGACCTGAACCTCCTCACTCGGAGGAAACTGAGTCGCCTGCTGGACCCGCCC
  GACCCCCTGGGGAAGGACTGGTGCCTTCTCGCCATGAACTTAGGCCTCCCTGACCTCGTG
  GCAAAGTACAACACCAATAACGGGGCTCCCAAGGATTTCCTCCCCAGCCCCCTCCACGCC
  CTGCTGCGGGAATGGACCACCTACCCTGAGAGCACAGTGGGCACCCTCATGTCCAAACTG
  AGGGAGCTGGGTCGCCGGGATGCCGCAGACTTTTTGCTGAAGGCATCCTCTGTGTTCAAA
  ATCAACCTGGATGGCAATGGCCAGGAGGCCTATGCCTCGAGCTGCAACAGCGGCACCTCT
  TACAATTCCATTAGCTCTGTTGTATCCCGGTGA
  
  Clone variation with respect to NM_004938.2
  125 a=>t;126 a=>g;1608 c=>t;3597 c=>t;4037 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004938 unedited
  CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCCGCGCAGAA
  CCCGCAGCGCCGGCCTGGCAGGGCAGCTCGGAGGTGGGTGGGCCGCGCCGCCAGCCCGCTTGCAGGGTCC
  CCATTGGCCGCCTGCCGGCCGCCCTCCGCCCAAAAGGCGGCAAGGAGCCGAGAGGCTGCTTCGGAGTGTG
  AGGAGGACAGCCGGACCGAGCCAACGCCGGGGACTTTGTTCCCTCCGCGGAGGGGACTCGGCAACTCGCA
  GCGGCAGGGTCTGGGGGCCCGGCGCCTGGGAGGGGATCTGCGCCCCCCCACTCACTCCCCTAGCTGTGTT
  CCCGCCGCCCGCCCCCGGCTAGTCTCCGGCGCTGGGGCCTATGGTCGCCCTCCGACGCCCCTCCGAAGGG
  ACCGGGGAACCTCCCAGGCCCCCGGAACTGGAAACGAATGCTTGAGGGGGCTTAGGGAGGCCCAGGACCG
  GTGGGGAGGTTCGGGAACGGGAGCGGAATGGCCCTGGGCTTTGGGGAGGCTGCACATTTAACTGTGCCCG
  TGTCTCGGCAGGAAACGCGTATAGATTCCACACCCCGAAAGCATTGGCCTGGGACATTTTGCGTGTAAAA
  AGTCCCTGAAAAGG
  
  Kinase domain sequence

  >SC323458 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CGCGTGCATTGGCAGTGGAAGTTTGCGGTTGTGAAGAAATGCCGTGAGAAAAGCACCGGCCTCCAGTATG
  CCGCCATGTTCATCAAGAAAAGGAGGACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGACATCGAGCG
  GGAGGTCAGCATCCTGAAGGAGATCCAGCACCCCAATGTCATCACCCTGCACGAGGTCTATGAGAACAAG
  ACGGACGTCATCCTGATCTTGGAACTCGTTGCAGGTGGCGAGCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_004938
• Insert Size: 6220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004938.1, NP_004929.1
• RefSeq Size: 5910 bp
• RefSeq ORF: 4296 bp
• Locus ID: 1612
• Cytogenetics: 9q21.33
• Domains: DEATH, pkinase, TyrKc, ANK, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Pathways in cancer
• Gene Summary: 'Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.