NUAK2 (NM_030952) Human Untagged Clone

CAT#: SC323459

NUAK2 (untagged)-Kinase deficient mutant (K81M) of Human NUAK family, SNF1-like kinase, 2 (NUAK2)


  "NM_030952" in other vectors (7)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NUAK2
Synonyms SNARK
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_030952, the custom clone sequence may differ by one or more nucleotides


ATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCCCGGCCGCTGG
CGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTGAAGCGGCACCACCACAAGCA
CAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGGCAAAGGCACCTACGGGAAGGTGAAGAAGGCG
CGGGAGAGCTCGGGGCGCCTGGTGGCCATCAAGTCAATCCGGAAGGACAAAATCAAAGATGAGCAAGATC
TGATGCACATACGGAGGGAGATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGA
AGTGTTTGAGAACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCCGGGGCGACCTTTATGACTAC
ATCAGCGAGCGGCAGCAGCTCAGTGAGCGCGAAGCTAGGCATTTCTTCCGGCAGATCGTCTCTGCCGTGC
ACTATTGCCATCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAACATCCTCTTGGATGCCAATGG
GAATATCAAGATTGCTGACTTCGGCCTCTCCAACCTCTACCATCAAGGCAAGTTCCTGCAGACATTCTGT
GGGAGCCCCCTCTATGCCTCGCCAGAGATTGTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCT
GGTCCCTGGGTGTTCTCCTCTACATCCTGGTGCATGGCACCATGCCCTTTGATGGGCATGACCATAAGAT
CCTAGTGAAACAGATCAGCAACGGGGCCTACCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATC
CGGTGGCTGTTGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCACTGGTGGGTCA
ACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCCGCATGAGGGTGGGCACCCTGGCAGTGACTC
TGCCCGCGCCTCCATGGCTGACTGGCTCCGGCGTTCCTCCCGCCCCCTCCTGGAGAATGGGGCCAAGGTG
TGCAGCTTCTTCAAGCAGCATGCACCTGGTGGGGGAAGCACCACCCCTGGCCTGGAGCGCCAGCATTCGC
TCAAGAAGTCCCGCAAGGAGAATGACATGGCCCAGTCTCTCCACAGTGACACGGCTGATGACACTGCCCA
TCGCCCTGGCAAGAGCAACCTCAAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGCCTCTGCAGAA
GGGGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTGCGAGCCCAGGGCAGGCTGCCCCGCTGCTCC
CCAAGAAGGGCATTCTCAAGAAGCCCCGACAGCGCGAGTCTGGCTACTACTCCTCTCCCGAGCCCAGTGA
ATCTGGGGAGCTCTTGGACGCAGGCGACGTGTTTGTGAGTGGGGATCCCAAGGAGCAGAAGCCTCCGCAA
GCTTCAGGGCTGCTCCTCCATCGCAAAGGCATCCTCAAACTCAATGGCAAGTTCTCCCAGACAGCCTTGG
AGCTCGCGGCCCCCACCACCTTCGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGGCCCGGGCCAG
CCGACCCTCAGGGGCTGTGAGCGAGGACAGCATCCTGTCCTCTGAGTCCTTTGACCAGCTGGACTTGCCT
GAACGGCTCCCAGAGCCCCCACTGCGGGGCTGTGTGTCTGTGGACAACCTCACGGGGCTTGAGGAGCCCC
CCTCAGAGGGCCCTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTGGGGGACAGCTGCTTTTCCCT
GACAGACTGCCAGGAGGTGACAGCGACCTACCGACAGGCACTGAGGGTCTGCTCAAAGCTCACCTGA


>OriGene 5' read for mutant NM_030952 unedited
CCGCCCGTTCTCAGCAAATGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAG
TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGCTCCCC
GTCCTGGTGCGGGACCTGTGCCCCGCGCTTCAGCCCTCCCCGCACAGCCTACTGATTCCCCTGCCGCCCT
TGCTCACCTCCTGCTCGCCATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCA
GAGCTAGCCCGGCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTGA
AGCGGCACCACCACAAGCACAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGCAAAGGCACCTAC
GGGAAGGTGAAGAGGCGCGGGAGAGCTCGGGCGCCTGTGGCCATCATGTCAATCCGGAGGACAAATTCAA
AGATGAGCAGATCTGATGCAACATACGGAGGGAGATTGAAGATCATTGTCATCACTCACCACCTTCCAAT
CATGCATCATGACTGTGTGGAAACAGCAGCAAGATCGGGATCGTCATGAATTATGCAGCTGGGCGGACTT
ATTAGACCTCACTACCGACGCGCACACCGTCTATGTAGACGCGGAGCTAGGCATTCTTCCGGCATATCGT
CTTTGGCTGTGCATATTGGCCCTCGAACAGATGTGCCCCGGATTCCACCTGGACACTCTTGTAGATCCAT
GGGAATACGAATTGAATCTGCCCTCCAACTTACCAAGGAATTCGGACATCTTTGGGAACCTTAGCGCTCG
AATGTAATGGAACCATCAAGCCAAGTGACAGTGCCGTGTTATCCTACGGTTAGCCAGCTTGGATCAGATC
TAGCATTACCGTCTCGAT
>SC323459 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYACKMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGCTCCCCGTCCTGGTG
CGGGACCTGTGCCCCGCGCTTCAGCCCTCCCCGCACAGCCTACTGATTCCCCTGCCGCCCTTGCTCACCT
CCTGCTCGCCATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCC
Restriction Sites Please inquire     
ACCN NM_030952
ORF Size 1887 bp
Insert Size 4000
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Reference Data
RefSeq NM_030952.1, NP_112214.1
RefSeq Size 3443
RefSeq ORF 1887
Locus ID 81788
Protein Families Druggable Genome, Protein Kinase

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