PCTAIRE2 (CDK17) (NM_002595) Human Untagged Clone

CAT#: SC323462

CDK17 (untagged)-Kinase deficient mutant (K221M) of Human PCTAIRE protein kinase 2 (PCTK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK17
• Synonyms: PCTAIRE2; PCTK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002595, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAAAAATTTAAGAGAAGGCTATCCCTCACACTCCGAGGAAGTCAGACTATTGATGAATCATTGTCTG
  AATTGGCTGAACAAATGACTATTGAAGAAAACAGCAGCAAGGATAATGAGCCTATTGTGAAGAATGGCAG
  GCCTCCAACGTCTCACAGTATGCATTCCTTCCTCCACCAGTACACAGGATCTTTCAAGAAGCCCCCATTG
  CGGAGACCACACAGTGTTATTGGAGGGAGCCTTGGCTCCTTCATGGCAATGCCCAGAAATGGAAGCAGAT
  TAGATATTGTTCATGAAAATCTAAAAATGGGATCAGATGGTGAGAGTGACCAAGCTTCTGGGACATCATC
  TGATGAAGTCCAGTCACCTACAGGTGTTTGTCTCAGAAATCGTATACATAGACGGATCTCAATGGAGGAT
  TTAAATAAGCGGTTATCACTGCCTGCAGACATCAGAATACCTGATGGATATCTTGAAAAGTTGCAGATAA
  ACAGTCCACCATTTGACCAACCAATGAGTCGAAGGTCTCGTAGAGCTTCCTTATCAGAAATTGGCTTTGG
  AAAAATGGAAACCTACATCAAATTGGAAAAGCTTGGAGAGGGTACATATGCAACAGTATATAAAGGAAGA
  AGTAAATTGACAGAGAATTTGGTGGCATTAAAAGAGATCCGATTGGAACATGAAGAAGGTGCACCCTGCA
  CAGCTATAAGAGAAGTTTCACTATTAAAGGATTTAAAACATGCAAATATAGTAACCTTACATGACATTGT
  TCACACAGATAAATCCTTGACTTTGGTGTTTGAGTATCTGGATAAAGACCTGAAACAGTACATGGATGAC
  TGTGGAAACATCATGAGTATGCACAACGTAAAGCTGTTTCTGTACCAAATTCTACGTGGTTTGGCATATT
  GCCATAGAAGAAAGGTATTGCATCGAGACTTGAAACCACAGAACCTCCTCATTAATGAGAAAGGAGAATT
  AAAGCTAGCAGATTTTGGACTAGCCCGAGCCAAGTCAGTTCCCACAAAGACCTACTCAAATGAAGTTGTC
  ACACTATGGTACCGGCCACCTGATGTGCTTCTTGGTTCCTCGGAGTACTCAACACAGATTGACATGTGGG
  GTGTTGGTTGCATTTTCTTTGAAATGGCTTCTGGAAGACCTTTATTTCCAGGATCAACCGTGGAAGATGA
  ACTGCACTTAATTTTCCGACTGCTAGGAACTCCATCTCAGGAAACTTGGCCAGGTATTTCTTCAAATGAG
  GAGTTCAAGAACTACAACTTTCCAAAATATAAACCACAGCCTCTAATTAACCACGCACCCAGGTTAGACT
  CTGAAGGAATTGAGTTGATAACAAAATTTCTTCAGTATGAATCTAAGAAAAGGGTTTCAGCTGAAGAGGC
  CATGAAACATGTGTACTTTCGAAGTCTGGGACCAAGAATACATGCTTTACCAGAAAGTGTATCAATATTC
  AGTTTGAAAGAGATTCAGTTGCAAAAGGACCCGGGTTTTCGAAATTCTTCTTATCCAGAGACAGGACATG
  GGAAGAACAGAAGACAGAGCATGCTCTTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002595 unedited
  ACGCCGTTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGAAAGCGACGCACA
  CGCAAGGAGCGAACGCGGCCGCCCGCAGTCTCCTGAGCTCCCCGCGGGCCGCCGCAGAGCCGCGGAGAAG
  GAGCCAGCGCCCGGCCAGACAGTCTCAGCCCGCCGTCGGCGCCCGCCGCGCTCGCGCCCGGCTGCTCCGA
  GGGGATTAGTGGGCCGTCCGCGCGGCTCGTCGGCGCGGGAGCCATGTTGACCGAGGGACTTTGGCAACGC
  GTGAGACACCCGCGGCCCCTCCCGGAGGCTCCGGACCCGCGGCCGAGAGCCCGGAAAGGCGGCTGGTGGC
  GGCCGGAAGCCCTGCCGCTCGGAGCCGCCTCCACGTTCTGCCGGCCGCTGAAAACCCGGACTGGCATCCG
  GTCCGCCCCGGGCGTCCGGCGGAACAGCTTTCCCCCGGCGCCTTCTTTCGCTTCGCCTCGGAACCGGGGG
  TCCCCGCTCTGGTGGAATTTTCAAGCCCATTCATTGTGTGGATGAAAATTTGAGAAGGGCATCCTCACCT
  CGGAAATCGACACTGAGAAACTATGCGAGATGGGCGACCATGCACTGAGAGACCGGCCGGGATGGGA
  
  Kinase domain sequence

  >SC323462 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TYWTGKKGAARYTGGAGAGGGTACATATGCAACAGTATATAAAGGAAGAAGTAAATTGACAGASMATTTG
  GTGGCATTAATGGAGATCCGATTGGAACATGAAGAAGGTGCACCCTGCACAGCTATAAGAGAAGTTTCAC
  TATTAAAGGATTTAAAACATGCAAATATAGTAACCTTACATGACATTGTTCACACAGATAAATCCTTGAC
  TTTGGTGTTTGAGTATCTGGATAAAGACCTGAAACAGTACATGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_002595
• ORF Size: 1572 bp
• Insert Size: 3900
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_002595.2, NP_002586.2
• RefSeq Size: 4041
• RefSeq ORF: 1572
• Locus ID: 5128
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It has similarity to a rat protein that is thought to play a role in terminally differentiated neurons. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1.