Casein Kinase 1 alpha (CSNK1A1) (NM_001892) Human Untagged Clone

CAT#: SC323470

CSNK1A1 (untagged)-Kinase deficient mutant (K46M) of Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSNK1A1
• Synonyms: CK1; CK1a; CKIa; HEL-S-77p; HLCDGP1; PRO2975
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323470 sequence for NM_001892 edited (data generated by NextGen Sequencing)
  ATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTCGGAGGGAAATATAAACTGGTA
  CGGAAGATCGGGTCTGGCTCCTTCGGGGACATCTATTTGGCGATCAACATCACCAACGGC
  GAGGAAGTGGCAGTGAAGCTAGAATCTCAGAAGGCCAGGCATCCCCAGTTGCTGTACGAG
  AGCAAGCTCTATAAGATTCTTCAAGGTGGGGTTGGCATCCCCCACATACGGTGGTATGGT
  CAGGAAAAAGACTACAATGTACTAGTCATGGATCTTCTGGGACCTAGCCTCGAAGACCTC
  TTCAATTTCTGTTCAAGAAGGTTCACAATGAAAACTGTACTTATGTTAGCTGACCAGATG
  ATCAGTAGAATTGAATATGTGCATACAAAGAATTTTATACACAGAGACATTAAACCAGAT
  AACTTCCTAATGGGTATTGGGCGTCACTGTAATAAGTTATTCCTTATTGATTTTGGTTTG
  GCCAAAAAGTACAGAGACAACAGGACAAGGCAACACATACCATACAGAGAAGATAAAAAC
  CTCACTGGCACTGCCCGATATGCTAGCATCAATGCACATCTTGGTATTGAGCAGAGTCGC
  CGAGATGACATGGAATCATTAGGATATGTTTTGATGTATTTTAATAGAACCAGCCTGCCA
  TGGCAAGGGCTAAAGGCTGCAACAAAGAAACAAAAATATGAAAAGATTAGTGAAAAGAAG
  ATGTCCACGCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTTGCGATGTACTTA
  AACTATTGTCGTGGGCTACGCTTTGAGGAAGCCCCAGATTACATGTATCTGAGGCAGCTA
  TTCCGCATTCTTTTCAGGACCCTGAACCATCAATATGACTACACATTTGATTGGACAATG
  TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA
  ACCCCCACAGGCAAGCAAACTGACAAAACCAAGAGTAACATGAAAGGTTTCTAA
  
  Clone variation with respect to NM_001892.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001892 unedited
  CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGGGGTCCCT
  CTGGGCCGGAGCGCGGCGGCAGTGGTGGCAGCGGTATCGCCGCCCTAGCTCACCGCGCCCCTTTTCCAGC
  CCGCGACGTCGCCGCGCAAGCGAGGCAGCGGCGGCCGCCGAGAAACAAGTGGCCCAGCCTGGTAACCGCC
  GAGAAGCCCTTCACAAACTGCGGCCTGGCAAAAAGAAACCTGACTGAGCGGCGGTGATCAGGTGTCCCGC
  TCTGCTGATTCTGGGCCCCGAACCCCCGGTAAAGGCCTCCGTGTTCCGTTTTCCTGCCGCCCCTCCTCCG
  TAGCCCTTGCCTATGTAGGACCCCCGAGGCCTCCGTCCTCTTCCCAGAGGTTGTCGGGGCTTGCCCCAGC
  CCTCCATCTTCGTTCTCTAAGGATGGCAAGTAGCAGCGGGTCCAAGGCTGATTTCATTGCCGGGAGGGAA
  TTTACCTGGTACGGAGATCGGGTCTGGTCCCTCGGGACATATTTTGGCGTTAACTCCCACGGAGGGAATG
  GGCATGATGCTAATCTAAAGGGCGAGCTCAATGTGTGTCAGAGACAGCTATAGAGATTTCAAGGGGGTGG
  CCCACCATCGTGTGATGACGGAACACAACAGATATCTCGGCGTA
  
  Kinase domain sequence

  >SC323470 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYATGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGGGGTCCCTCTGGGCC
  GGAGCCGGCGGCAGTGGTGGCAGCGGTATCGCCGCCCTAGCTCACCGCGCCCCTTTTCCAGCCCGCGACG
  TCGCCGCGCAAGCGAGGCAGCGGCGGCCGCCGAGAAACAAGTGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_001892
• Insert Size: 2630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001892.4, NP_001883.4
• RefSeq Size: 3065 bp
• RefSeq ORF: 1014 bp
• Locus ID: 1452
• Cytogenetics: 5q32
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Hedgehog signaling pathway, Wnt signaling pathway
• Gene Summary: ''
  Transcript Variant: This variant (2) lacks an in-frame internal exon, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.