Casein Kinase 1 alpha (CSNK1A1) (NM_001892) Human Untagged Clone

CAT#: SC323470

CSNK1A1 (untagged)-Kinase deficient mutant (K46M) of Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2


  "NM_001892" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CSNK1A1
Synonyms CK1; CK1a; CKIa; HEL-S-77p; HLCDGP1; PRO2975
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323470 sequence for NM_001892 edited (data generated by NextGen Sequencing)
ATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTCGGAGGGAAATATAAACTGGTA
CGGAAGATCGGGTCTGGCTCCTTCGGGGACATCTATTTGGCGATCAACATCACCAACGGC
GAGGAAGTGGCAGTGAAGCTAGAATCTCAGAAGGCCAGGCATCCCCAGTTGCTGTACGAG
AGCAAGCTCTATAAGATTCTTCAAGGTGGGGTTGGCATCCCCCACATACGGTGGTATGGT
CAGGAAAAAGACTACAATGTACTAGTCATGGATCTTCTGGGACCTAGCCTCGAAGACCTC
TTCAATTTCTGTTCAAGAAGGTTCACAATGAAAACTGTACTTATGTTAGCTGACCAGATG
ATCAGTAGAATTGAATATGTGCATACAAAGAATTTTATACACAGAGACATTAAACCAGAT
AACTTCCTAATGGGTATTGGGCGTCACTGTAATAAGTTATTCCTTATTGATTTTGGTTTG
GCCAAAAAGTACAGAGACAACAGGACAAGGCAACACATACCATACAGAGAAGATAAAAAC
CTCACTGGCACTGCCCGATATGCTAGCATCAATGCACATCTTGGTATTGAGCAGAGTCGC
CGAGATGACATGGAATCATTAGGATATGTTTTGATGTATTTTAATAGAACCAGCCTGCCA
TGGCAAGGGCTAAAGGCTGCAACAAAGAAACAAAAATATGAAAAGATTAGTGAAAAGAAG
ATGTCCACGCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTTGCGATGTACTTA
AACTATTGTCGTGGGCTACGCTTTGAGGAAGCCCCAGATTACATGTATCTGAGGCAGCTA
TTCCGCATTCTTTTCAGGACCCTGAACCATCAATATGACTACACATTTGATTGGACAATG
TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA
ACCCCCACAGGCAAGCAAACTGACAAAACCAAGAGTAACATGAAAGGTTTCTAA

Clone variation with respect to NM_001892.4
>OriGene 5' read for mutant NM_001892 unedited
CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGGGGTCCCT
CTGGGCCGGAGCGCGGCGGCAGTGGTGGCAGCGGTATCGCCGCCCTAGCTCACCGCGCCCCTTTTCCAGC
CCGCGACGTCGCCGCGCAAGCGAGGCAGCGGCGGCCGCCGAGAAACAAGTGGCCCAGCCTGGTAACCGCC
GAGAAGCCCTTCACAAACTGCGGCCTGGCAAAAAGAAACCTGACTGAGCGGCGGTGATCAGGTGTCCCGC
TCTGCTGATTCTGGGCCCCGAACCCCCGGTAAAGGCCTCCGTGTTCCGTTTTCCTGCCGCCCCTCCTCCG
TAGCCCTTGCCTATGTAGGACCCCCGAGGCCTCCGTCCTCTTCCCAGAGGTTGTCGGGGCTTGCCCCAGC
CCTCCATCTTCGTTCTCTAAGGATGGCAAGTAGCAGCGGGTCCAAGGCTGATTTCATTGCCGGGAGGGAA
TTTACCTGGTACGGAGATCGGGTCTGGTCCCTCGGGACATATTTTGGCGTTAACTCCCACGGAGGGAATG
GGCATGATGCTAATCTAAAGGGCGAGCTCAATGTGTGTCAGAGACAGCTATAGAGATTTCAAGGGGGTGG
CCCACCATCGTGTGATGACGGAACACAACAGATATCTCGGCGTA
>SC323470 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYATGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGGGGTCCCTCTGGGCC
GGAGCCGGCGGCAGTGGTGGCAGCGGTATCGCCGCCCTAGCTCACCGCGCCCCTTTTCCAGCCCGCGACG
TCGCCGCGCAAGCGAGGCAGCGGCGGCCGCCGAGAAACAAGTGGC
Restriction Sites Please inquire     
ACCN NM_001892
Insert Size 2630 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001892.4, NP_001883.4
RefSeq Size 3065 bp
RefSeq ORF 1014 bp
Locus ID 1452
Cytogenetics 5q32
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
Protein Pathways Hedgehog signaling pathway, Wnt signaling pathway
Gene Summary ''
Transcript Variant: This variant (2) lacks an in-frame internal exon, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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