DRAK2 (STK17B) (NM_004226) Human Untagged Clone

CAT#: SC323478

STK17B (untagged)-Kinase deficient mutant (K62M) of Human serine/threonine kinase 17b (STK17B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK17B
• Synonyms: DRAK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323478 sequence for NM_004226 edited (data generated by NextGen Sequencing)
  ATGTCGAGGAGGAGATTTGATTGCCGAAGTATTTCAGGCCTACTAACTACAACTCCTCAA
  ATTCCAATAAAAATGGAAAACTTTAATAATTTCTATATACTTACATCTAAAGAGCTAGGG
  AGAGGTAAATTTGCTGTGGTTAGACAATGTATATCAAAATCTACTGGCCAAGAATATGCT
  GCAATGTTTCTAAAAAAGAGAAGAAGAGGACAGGATTGTCGGGCAGAAATTTTACACGAG
  ATTGCTGTGCTTGAATTGGCAAAGTCTTGTCCCCGTGTTATTAATCTTCATGAGGTCTAT
  GAAAATACAAGTGAAATCATTTTGATATTGGAATATGCTGCAGGTGGAGAAATTTTCAGC
  CTGTGTTTACCTGAGTTGGCTGAAATGGTTTCTGAAAATGATGTTATCAGACTCATTAAA
  CAAATACTTGAAGGAGTTTATTATCTACATCAGAATAACATTGTACACCTTGATTTAAAG
  CCACAGAATATATTACTGAGCAGCATATACCCTCTCGGGGACATTAAAATAGTAGATTTT
  GGAATGTCTCGAAAAATAGGGCATGCGTGTGAACTTCGGGAAATCATGGGAACACCAGAA
  TATTTAGCTCCAGAAATCCTGAACTATGATCCCATTACCACAGCAACAGATATGTGGAAT
  ATTGGTATAATAGCATATATGTTGTTAACTCACACATCACCATTTGTGGGAGAAGATAAT
  CAAGAAACATACCTCAATATCTCTCAAGTTAATGTAGATTATTCGGAAGAAACTTTTTCA
  TCAGTTTCACAGCTGGCCACAGACTTTATTCAGAGCCTTTTAGTAAAAAATCCAGAGAAA
  AGACCAACAGCAGAGATATGCCTTTCTCATTCTTGGCTACAGCAGTGGGACTTTGAAAAC
  TTGTTTCACCCTGAAGAAACTTCCAGTTCCTCTCAAACTCAGGATCATTCTGTAAGGTCC
  TCTGAAGACAAGACTTCTAAATCCTCCTGTAATGGAACCTGTGGTGATAGAGAAGACAAA
  GAGAATATCCCAGAGGATAGCAGCATGGTTTCCAAAAGATTTCGTTTCGATGACTCATTA
  CCCAATCCCCATGAACTTGTTTCAGATTTGCTCTGTTAG
  
  Clone variation with respect to NM_004226.3
  126 a=>t;185 a=>t;186 a=>g;222 a=>g;741 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004226 unedited
  ACGCCCGTCCAGCAACGGGCGGTAGGCGCTGCACGGTGGGAGGTTCTATATAAGCAGAGCCCGTCTAGCG
  AACCGTCAGAATCCCGTAATACGACCCACTATAGGGCGGCCGCGAATCCGGCACGAGGGCCCTCCCCGCT
  GCTGTCGCCAGGAGACACTTTCACGAGAAGCCAGGTCACAACCGTCGGCCCTTTGTCTGGAAAAGCAAAA
  GTGGATCCTGCCACGTTCGGAGCTCCCTGGCGCCTCGCCCGGCTGGAGCTAGAGAACTCGTCCTGTGGCG
  GCCCCCGGCGTGGGGCGGGACAGCGGCCCCCTGGAGGGGGCAGTCCCGGGAGAACCTGCGGCGGCCGGAG
  CGGTAAAAATAAGTGACTAAAGAGCAGACCTGGGATCACCTAACATGTCGAGGAAGAGATTTGATTGCCG
  AGTATTTCAGGCCTACTACCTACCAACTCCTCAAATTCAATAAAAAATGAAAACTTTAATAATTTCTAAT
  ATACCTACCATCCTAAGGAGCTAGGGAAGAGGTAAATTTGCTTTGGTTAGAACATGGTATTCAAAATCTA
  ACTGCAAGAATATGCCTGCAATTGTTCTAAAAGAGAGAAAAGGACGGATTGTCCGGCAAAATTTAACCGA
  ATGCGTGCTGAATTGGCAAGTCTGTCCCGGTATAACTTCTGAGGCTTGAATCATGAAACTTGATGGAATG
  CCGTGGGAATACCGGTTACGATGCGAAGGTTCGGATAGTTCAACTACACTAGACTAATCCGAACTGACGG
  GTGAAGCGGATTCAGACAAAATCCGCATAATAG
  
  Kinase domain sequence

  >SC323478 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TCTTACATCTAATGAGCTAGGGAGAGGTAAATTTGCTGTGGTTAGACAATGTATATCAAAATCTACTGGC
  CAAGAATATGCTGCAATGTTTCTAAAAAAGAGAAGAAGAGGACAGGATTGTCGGGCAGAAATTTTACACG
  AGATTGCTGTGCTTGAATTGGCAAAGTCTTGTCCCCGTGTTATTAATCTTCATGAGGTCTATGAAAATAC
  AAGTGAAATCATTTTGATATTGGAATATGCTGCAGGTGGAGAAAT
  
• Restriction Sites: Please inquire
• ACCN: NM_004226
• ORF Size: 1119 bp
• Insert Size: 2550
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_004226.2, NP_004217.1
• RefSeq Size: 1707
• RefSeq ORF: 1119
• Locus ID: 9262
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase