PBK (NM_018492) Human Untagged Clone

CAT#: SC323480

PBK (untagged)-Kinase deficient mutant (K64M) of Human PDZ binding kinase (PBK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PBK
• Synonyms: CT84; HEL164; Nori-3; SPK; TOPK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323480 sequence for NM_018492 edited (data generated by NextGen Sequencing)
  ATGGAAGGGATCAGTAATTTCAAGACACCAAGCAAATTATCAGAAAAAAAGAAATCTGTA
  TTATGTTCAACTCCAACTATAAATATCCCGGCCTCTCCGTTTATGCAGAAGCTTGGCTTT
  GGTACTGGGGTAAATGTGTACCTAATGAAAAGATCTCCAAGAGGTTTGTCTCATTCTCCT
  TGGGCTGTAATGAAGATTAATCCTATATGTAATGATCATTATCGAAGTGTGTATCAAAAG
  AGACTAATGGATGAAGCTAAGATTTTGAAAAGCCTTCATCATCCAAACATTGTTGGTTAT
  CGTGCTTTTACTGAAGCCAGTGATGGCAGTCTGTGTCTTGCTATGGAATATGGAGGTGAA
  AAGTCTCTAAATGACTTAATAGAAGAACGATATAAAGCCAGCCAAGATCCTTTTCCAGCA
  GCCATAATTTTAAAAGTTGCTTTGAATATGGCAAGAGGGTTAAAGTATCTGCACCAAGAA
  AAGAAACTGCTTCATGGAGACATAAAGTCTTCAAATGTTGTAATTAAAGGCGATTTTGAA
  ACAATTAAAATCTGTGATGTAGGAGTCTCTCTACCACTGGATGAAAATATGACTGTGACT
  GACCCTGAGGCTTGTTACATTGGCACAGAGCCATGGAAACCCAAAGAAGCTGTGGAGGAG
  AATGGTGTTATTACTGACAAGGCAGACATATTTGCCTTTGGCCTTACTTTGTGGGAAATG
  ATGACTTTATCGATTCCACACATTAATCTTTCAAATGATGATGATGATGAAGATAAAACT
  TTTGATGAAAGTGATTTTGATGATGAAGCATACTATGCAGCCTTGGGAACTAGGCCACCT
  ATTAATATGGAAGAACTGGATGAATCATACCAGAAAGTAATTGAACTCTTCTCTGTATGC
  ACTAATGAAGACCCTAAAGATCGTCCTTCTGCTGCACACATTGTTGAAGCTCTGGAAACA
  GATGTCTAG
  
  Clone variation with respect to NM_018492.2
  191 a=>t;192 a=>g;320 a=>g;822 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_018492 unedited
  ACCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGTGTTGGTGCT
  AGAGGCAGCTGCAGGGTCTCGCTGGGGGCCGCTCGGGACCAATTTTGAAGAGGTACTTGGCCACGACTTA
  TTTTCACCTCCGACCTTTCCTTCCAGGCGGTGAGACTCTGGACTGAGAGTGGCTTTCACAATGGAAGGGA
  TCAGTAATTTCAAGACACCAAGCAAATTATCAGAAAAAAAAGAAATCTGTATTATGTTCAACTCCAACTA
  TAAAATATCCCCGGCCTCTCCGTTATATGCAGGAGGCTTTGGCTTTTGGTACCTGGGGGTAAATGGGTGT
  ACCCTAAATGAAAAAATTTCTCCCAAGAAGGTTTTGTCTTCATTTCTCCTTGGGGCTTGTATGGAAAAAT
  AAAATCCCATATATGTTAAGGATCCATATATCGGAAGTGGTGTTTTCAAAAAAAAAACTAAGGGGATAAG
  CCCTAAAAATTTTTGAAAAAGCCCTTTATTCATCCCAAACCTTGGTGGGGTATATCGGCGCTTTTACCGG
  AACCACTGGAAGGGGATCTCTGTGTTTTGGATAGGGAATTATGGAATGGAAAAAAGTTCTCTATGAGCTT
  TAATTAAAAAAACCGATTATAGCCCCCCGGCCCAATTTTCCTTTTTCCCAGCCCCCATTTTTTTTTAAAA
  TGTTTGCTTTTATATATGTGGGGAAGAGAGTGTTAAATATTTTGCGCGCCCCAAAGAAGAAGACACGCGT
  GCTTGGAGAGCACAAAAAACCTTCTCACAGATGTGTTGATATATAAGCGACGCTTTGTGACACACAACAA
  AATGTGGTGAGAGGAGGATCTCCTTCCCCCACGCGTGGAAGAATAGTTGTGATGGAGACCACGCACGGCT
  GCTTATAGTGGCGCACCACTCGTGCACACAAACTCTTGTGTCAGAGAAGCGTTTTATTATGCAG
  
  Kinase domain sequence

  >SC323480 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYAATGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGTGTTGGTGCTAGAGGC
  AGCTGCAGGGTCTCGCTGGGGGCCGCTCGGGACCAATTTTGAAGAGGTACTTGGCCACGACTTATTTTCA
  CCTCCGACCTTTCCTTCCAGGCGGTGAGACTCTGGACTGAGAGTG
  
• Restriction Sites: Please inquire
• ACCN: NM_018492
• ORF Size: 969 bp
• Insert Size: 1850
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_018492.2, NP_060962.2
• RefSeq Size: 1899
• RefSeq ORF: 969
• Locus ID: 55872
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) represents the longest transcript, and encodes the shorter isoform (1). Variants 1 and 3 encode the same isoform (1).