MAPK15 (NM_139021) Human Untagged Clone

CAT#: SC323484

MAPK15 (untagged)-Kinase deficient mutant (K42M) of Human mitogen-activated protein kinase 15 (MAPK15)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPK15
• Synonyms: ERK7; ERK8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139021, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAGCTCGGGCAGGGGGCCT
  ATGGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGC
  TTTTAGGGATAAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGAGTTTGGGGACCAT
  CCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAGAGAACGACAGGGACATTTACCTGGTGTTTGAGT
  TTATGGACACTGACCTGAACGCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTCCAT
  CTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACGTTGTGCACCGGGACCAGAAGCCG
  TCCAATGTGCTCCTGGATGCCAACTGCACAGTGAAGCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCG
  ACCTCCCCGAGGGGCCTGAGGACCAGGCCGTGACAGAGTACGTGGCCACACGCTGGTACCGAGCACCGGA
  GGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTGGACATGTGGAGTCTGGGCTGTATCCTGGGGGAG
  ATGCTGCGGGGGAGACCCCTGTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGACCA
  TCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGCCGTGCCTCTGTGCTGCACCAGCT
  GGGGTCCCGGCCACGACAGACGCTGGATGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTC
  CTTAGGCGACTCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTGCAGCACCCCTACG
  TGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACGAGAGGCAGATGTGCGGCCCCGGGCACACGAAGG
  GGTCCAGCTCTCTGTGCCTGAGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGCAGC
  GGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGCACACCTGCACAAACCCAGAGCCG
  ACCCTCAGCTGCCTTCTAGGACACCTGTGCAGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGA
  CCCTGCCGAGCACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGCTCCCCTGCTCCAA
  ACTGCTCTCCTAGGGAATGGGGAAAGGCCCCCTGGGGCGAAGGAAGCGCCCCCCTTGACACTCTCGCTGG
  TGAAGCCAAGCGGGAGGGGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCAGGC
  CCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCAGCGTACAACAGGTCCCTCCCCGG
  CTTCCTCCGGAGGCCCGGCCCGGCCGGAGGATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTG
  CCAGGGCTTTGCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCACTGGGCCACCTGCC
  CCTGCTGGAGGGGCACCATGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_139021 unedited
  CCCGCCCGTCTCAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATATTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATCGACTCAACAGT
  AAGGCCCCGCGGGCGTCCTGGCCGCCATGTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACT
  CAGGCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAGGTC
  GTGGCCATCATGAAAATCTTTGATGCTTTTTAGGGATAAGACAGATGCCCCAGAGAACATTTCCGGGAAA
  TCACGCTCCCTCCCAGGAGTTTTGGGGGACCCATCCCCAACATCATCAGCCCTCCTTGACGTTGATCCCG
  GGCAGAAGAACGAACAGGGACATTTTACCCTGGTTGTTTGAAGTTTTATGGTTTGCCCCCCCCAGCCCCC
  CCACCCCCGGACTGGAGTTGGCGCACCCTTCTGCAAACCTGACCTGAACGCAGTATTCCGGAAGGGCCGG
  CCGGGTGAGGAACTTCCCGTGCGTCACTTTTCACAAGTTCGGGGGCCACCGGTTCCTCACTCGGGAATGT
  GTGCCCGGACCAACCTCCAATGGTCTCGGATGCATTGCATGAACCTTGAATTGCCGGCGCGCCGGGAACT
  CCTGAGGCCTGGCCGCCGTGAAATCTTGCCACCGATCACACGGGGGGGCT
  
  Kinase domain sequence

  >SC323484 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSATKMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATCGACTCAACAGTAAGGCCCCGC
  GGGCGTCCTGGCCGCCATGTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAG
  CTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACCGG
  
• Restriction Sites: Please inquire
• ACCN: NM_139021
• ORF Size: 834 bp
• Insert Size: 2328
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_139021.1, NP_620590.1
• RefSeq Size: 1904
• RefSeq ORF: 834
• Locus ID: 225689
• Protein Families: Druggable Genome, Protein Kinase