MYLK2 (NM_033118) Human Untagged Clone

CAT#: SC323498

MYLK2 (untagged)-Kinase deficient mutant (K314M) of Human myosin light chain kinase 2 (MYLK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYLK2
• Synonyms: KMLC; MLCK; MLCK2; skMLCK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323498 sequence for NM_033118 edited (data generated by NextGen Sequencing)
  ATGGCGACAGAAAATGGAGCAGTTGAGCTGGGAATTCAGAACCCATCAACAGACAAGGCA
  CCTAAAGGTCCCACAGGTGAAAGACCCCTGGCTGCAGGGAAAGACCCTGGCCCCCCAGAC
  CCAAAGAAAGCTCCGGATCCACCCACCCTGAAGAAAGATGCCAAAGCCCCTGCCTCAGAG
  AAAGGGGATGGTACCCTGGCCCAACCCTCAACTAGCAGCCAAGGCCCCAAAGGAGAGGGT
  GACAGGGGCGGGGGGCCCGCGGAGGGCAGTGCTGGGCCCCCGGCAGCCCTGCCCCAGCAG
  ACTGCGACACCTGAGACCAGCGTCAAGAAGCCCAAGGCTGAGCAGGGAGCCTCAGGCAGC
  CAGGATCCTGGAAAGCCCAGGGTGGGCAAGAAGGCAGCAGAGGGCCAAGCAGCAGCCAGG
  AGGGGCTCACCTGCCTTTCTGCATAGCCCCAGCTGTCCTGCCATCATCTCCAGTTCTGAG
  AAGCTGCTGGCCAAGAAGCCCCCAAGCGAGGCATCAGAGCTCACCTTTGAAGGGGTGCCC
  ATGACCCACAGCCCCACGGATCCCAGGCCAGCCAAGGCAGAAGAAGGAAAGAACATCCTG
  GCAGAGAGCCAGAAGGAAGTGGGAGAGAAAACCCCAGGCCAGGCTGGCCAGGCTAAGATG
  CAAGGGGACACCTCGAGGGGGATTGAGTTCCAGGCTGTTCCCTCAGAGAAATCCGAGGTG
  GGGCAGGCCCTCTGTCTCACAGCCAGGGAGGAGGACTGCTTCCAGATTTTGGATGATTGC
  CCGCCACCTCCGGCCCCCTTCCCTCACCGCATGGTGGAGCTGAGGACCGGGAATGTCAGC
  AGTGAATTCAGTATGAACTCCAAGGAGGCGCTCGGAGGTGGCAAGTTTGGGGCAGTCTGT
  ACCTGCATGGAGAAAGCCACAGGCCTCAAGCTGGCAGCCATGGTCATCAAGAAACAGACT
  CCCAAAGACAAGGAAATGGTGTTGCTGGAGATTGAGGTCATGAACCAGCTGAACCACCGC
  AATCTGATCCAGCTGTATGCAGCCATCGAGACTCCGCATGAGATCGTCCTGTTCATGGAG
  TACATCGAGGGCGGAGAGCTCTTCGAGAGGATTGTGGATGAGGACTACCATCTGACCGAG
  GTGGACACCATGGTGTTTGTCAGGCAGATCTGTGACGGGATCCTCTTCATGCACAAGATG
  AGGGTTTTGCACCTGGACCTCAAGCCAGAGAACATCCTGTGTGTCAACACCACCGGGCAT
  TTGGTGAAGATCATTGACTTTGGCCTGGCACGGAGGTATAACCCCAACGAGAAGCTGAAG
  GTGAACTTTGGGACCCCAGAGTTCCTGTCACCTGAGGTGGTGAATTATGACCAAATCTCC
  GATAAGACAGACATGTGGAGTATGGGGGTGATCACCTACATGCTGCTGAGCGGCCTCTCC
  CCCTTCCTGGGAGATGATGACACAGAGACCCTAAACAACGTTCTATCTGGCAACTGGTAC
  TTTGATGAAGAGACCTTTGAGGCCGTATCAGACGAGGCCAAAGACTTTGTCTCCAACCTC
  ATCGTCAAGGACCAGAGGGCCCGGATGAACGCTGCCCAGTGTCTCGCCCATCCCTGGCTC
  AACAACCTGGCGGAGAAAGCCAAACGCTGTAACCGACGCCTTAAGTCCCAGATCTTGCTT
  AAGAAATACCTCATGAAGAGGCGCTGGAAGAAAAACTTCATTGCTGTCAGCGCTGCCAAC
  CGCTTCAAGAAGATCAGCAGCTCGGGGGCACTGATGGCTCTGGGGGTCTGA
  
  Clone variation with respect to NM_033118.3
  941 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_033118 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCTGAGCAGCC
  GCTGGGAGACAGACGGCAACCAGGTTGCCCCTCTTTGCTCCAGCTAGAAAGACTTGAGTTAGACAAGCAG
  CAGCACACGCCTCCCTACCTCATGGCGACAGAAAATGGAGCAGTTGAGCTGGGAATTCAGAACCCATCAA
  CAGACAAGGCACCTAAAGGTCCCACAGGTGAAAAGACCCCTGGCTGCAGGGAAAAGACCCTGGCCCCCCC
  AGAACCCAAAGAAAGCTCCGGATCCCACCCCACCCCTGAAAGAAAAGAATGCCAAAGCCCCCTGCCTCAA
  GAGAAAAGGGGATGGTACCCTTGCCCCACCCCTCCACTCTAGCCAGCCCAAGGCCCCCAAAGGAAGAGGG
  TGAACAGGGGCCGGGGGGGGCCCGGCGGGAGGGACATGGCTGGGCCCCCGGGCAGCCTGGCCCCAGCAAA
  CTGGAACCCTGGAAACCAAGCGTAGAAAGCCCAGGGCTAAGCAGGGGACCCTCAGGCACCCGATATCCTG
  GAAAGCCCAGGGTGGGCAAAAAGCGCACAAAGGCCCACGCACACCCGAGAGGGGCTCATGGGTTTTTCGC
  AAACCCACTGTGTTGTGCTATCTTCTCGTTTCTAGAAATGCTGTGCCAAGACACCCCGCGAAGGGTCTCA
  GCTCTCCACTTTAGAGTGCCCCGAACCCCACGCGCACGGTATCACGCGCGCAGAGGAGAAGAGGAGACTC
  TTCTGAAACCCATGAGGATGTGAAAAACACCGCAGCTGTCGCATAATATACGGGGACACTCTGGGAGTGT
  AATGCGGTCCTCAGATCACTCAGTGCGAGCGCTGCTCACACGAGAGAGACTCGCATAGGTGTATCCGC
  
  Kinase domain sequence

  >SC323498 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AYTARRGYRGCSTCGGAGGTGGCAGTTTGGGGCAGTCTGTACCTGCATGGAGAAAGCCACAGGCCTCAAG
  CTGGCAGCCATGGTCATCAAGAAACAGACTCCCAAAGACAAGGAAATGGTGTTGCTGGAGATTGAGGTCA
  TGAACCAGCTGAACCACCGCAATCTGATCCAGCTGTATGCAGCCATCGAGACTCCGCATGAGATCGTCCT
  GTTCATGGAGTACATCGAGGGCGGAGAGCTCTTCGAGAGGATTGT
  
• Restriction Sites: Please inquire
• ACCN: NM_033118
• ORF Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_033118.2, NP_149109.1
• RefSeq Size: 2683
• RefSeq ORF: 1791
• Locus ID: 85366
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle. [provided by RefSeq, Jul 2008]