SGK1 (NM_005627) Human Untagged Clone

CAT#: SC323500

SGK1 (untagged)-Kinase deficient mutant (K127M) of Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SGK1
• Synonyms: SGK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323500 sequence for NM_005627 edited (data generated by NextGen Sequencing)
  ATGACGGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGGGCATG
  GTGGCAATTCTCATCGCTTTCATGAAGCAGAGGAGGATGGGTCTGAACGACTTTATTCAG
  AAGATTGCCAATAACTCCTATGCATGCAAACACCCTGAAGTTCAGTCCATCTTGAAGATC
  TCCCAACCTCAGGAGCCTGAGCTTATGAATGCCAACCCTTCTCCTCCACCAAGTCCTTCT
  CAGCAAATCAACCTTGGCCCGTCGTCCAATCCTCATGCTAAACCATCTGACTTTCACTTC
  TTGAAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGGCAGAA
  GAAGTGTTCTATGCAGTCAAAGTTTTACAGAAGAAAGCAATCCTGAAAAAGAAAGAGGAG
  AAGCATATTATGTCGGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTG
  GGCCTTCACTTCTCTTTCCAGACTGCTGACAAATTGTACTTTGTCCTAGACTACATTAAT
  GGTGGAGAGTTGTTCTACCATCTCCAGAGGGAACGCTGCTTCCTGGAACCACGGGCTCGT
  TTCTATGCTGCTGAAATAGCCAGTGCCTTGGGCTACCTGCATTCACTGAACATCGTTTAT
  AGAGACTTAAAACCAGAGAATATTTTGCTAGATTCACAGGGACACATTGTCCTTACTGAC
  TTCGGACTCTGCAAGGAGAACATTGAACACAACAGCACAACATCCACCTTCTGTGGCACG
  CCGGAGTATCTCGCACCTGAGGTGCTTCATAAGCAGCCTTATGACAGGACTGTGGACTGG
  TGGTGCCTGGGAGCTGTCTTGTATGAGATGCTGTATGGCCTGCCGCCTTTTTATAGCCGA
  AACACAGCTGAAATGTACGACAACATTCTGAACAAGCCTCTCCAGCTGAAACCAAATATT
  ACAAATTCCGCAAGACACCTCCTGGAGGGCCTCCTGCAGAAGGACAGGACAAAGCGGCTC
  GGGGCCAAGGATGACTTCATGGAGATTAAGAGTCATGTCTTCTTCTCCTTAATTAACTGG
  GATGATCTCATTAATAAGAAGATTACTCCCCCTTTTAACCCAAATGTGAGTGGGCCCAAC
  GACCTACGGCACTTTGACCCCGAGTTTACCGAAGAGCCTGTCCCCAACTCCATTGGCAAG
  TCCCCTGACAGCGTCCTCGTCACAGCCAGCGTCAAGGAAGCTGCCGAGGCTTTCCTAGGC
  TTTTCCTATGCGCCTCCCACGGACTCTTTCCTCTGA
  
  Clone variation with respect to NM_005627.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005627 unedited
  CCCGCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGAGCCGGTCT
  TTGAGCGCTAACGTCTTTCTGTCTCCCCGCGGTGGTGATGACGGTGAAAACTGAGGCTGCTAAGGGCACC
  CTCACTTACTCCAGGATGAGGGGCATGGTGGCAATTCTCATCGCTTTCATGAAGCAGAGGAGGATGGGTC
  TGAACGACTTTATTCAGAAGATTGCCAATAACTCCTATGCATGCAAACACCCTGAAGTTCAGTCCATCTT
  GAAGATCTCCCACCCTCAGGAGCCTGAGCTTATGATGCCACCCTTCTCCTCCACCAAGTCCTTCTCAGCA
  AATCACCTTGCCCGTCGTCAATCCTCATGCTAAACATCTGACTTCACTTCTTGAAAGTGATCGAAAGGCA
  GTTTGAAAGGTCTTCTAGCAGAACCAGGAGAAGAGGGTTCATCATCATGGTTTTAGAGAAAGCACCTGAA
  AAGAAAAGGGAAACCATTTATTCGAACGGATGTCGTGTAAAAGGAACCCCCTTCGTGTGGCTTCTTCTTT
  CGAATGGTGAAATGTACTTGCCAACACATAAGTGGAATTGTCTCACTCCAGAACGCTCTCGACCCCGCCC
  CTCTACCCTGAATCAGCCTGGCACGTCTGATCGTTAAGACTAACCAATTCGATCCCGACACTGCCTAGCT
  GCTAGGACTGACCGACTCCTTGACGAATCCTAGGCTGAACTAACCGAAGTGG
  
  Kinase domain sequence

  >SC323500 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYMTGCTAACATCTGACTTTMCTTCTTGAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGCAA
  GACACAAGGCAGAAGAAGTGTTCTATGCAGTCATGGTTTTACAGAAGAAAGCAATCCTGAAAAAGAAAGA
  GGAGAAGCATATTATGTCGGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTT
  CACTTCTCTTTCCAGACTGCTGACAAATTGTACTTTGTCCTAGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_005627
• ORF Size: 1296 bp
• Insert Size: 2560
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_005627.2, NP_005618.2
• RefSeq Size: 2386
• RefSeq ORF: 1296
• Locus ID: 6446
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the shortest isoform (1).