PCTAIRE1 (CDK16) (NM_006201) Human Untagged Clone

CAT#: SC323507

CDK16 (untagged)-Kinase deficient mutant (K194M) of Human PCTAIRE protein kinase 1 (PCTK1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK16
• Synonyms: PCTAIRE; PCTAIRE1; PCTGAIRE; PCTK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323507 sequence for NM_006201 edited (data generated by NextGen Sequencing)
  ATGGATCGGATGAAGAAGATCAAACGGCAGCTGTCAATGACACTCCGAGGTGGCCGAGGC
  ATAGACAAGACCAATGGTGCCCCTGAGCAGATAGGCCTGGATGAGAGTGGTGGTGGTGGC
  GGCAGTGACCCTGGAGAGGCCCCCACACGTGCTGCTCCTGGGGAACTTCGTTCTGCACGG
  GGCCCACTCAGCTCTGCACCAGAGATTGTGCACGAGGACTTGAAGATGGGGTCTGATGGG
  GAGAGTGACCAGGCTTCAGCCACGTCCTCGGATGAGGTGCAGTCTCCAGTGAGAGTGCGT
  ATGCGCAACCATCCCCCACGCAAGATCTCCACTGAGGACATCAACAAGCGCCTATCACTA
  CCAGCTGACATCCGGCTGCCTGAGGGCTACCTGGAGAAGCTGACCCTCAATAGCCCCATC
  TTTGACAAGCCCCTCAGCCGCCGCCTCCGTCGTGTCAGCCTATCTGAGATTGGCTTTGGG
  AAACTGGAGACCTACATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGTCTAC
  AAAGGCAAAAGCAAGCTCACAGACAACCTTGTGGCACTCATGGAGATCAGACTGGAACAT
  GAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTGTCCCTGCTCAAGGACCTCAAACAC
  GCCAACATCGTTACGCTACATGACATTATCCACACGGAGAAGTCCCTCACCCTTGTCTTT
  GAGTACCTGGACAAGGACCTGAAGCAGTACCTGGATGACTGTGGGAACATCATCAACATG
  CACAACGTGAAACTGTTCCTGTTCCAGCTGCTCCGTGGCCTGGCCTACTGCCACCGGCAG
  AAGGTGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACGAGAGGGGAGAGCTC
  AAGCTGGCTGACTTTGGCCTGGCCCGAGCCAAGTCAATCCCAACAAAGACATACTCCAAT
  GAGGTGGTGACACTGTGGTACCGGCCCCCTGACATCCTGCTTGGGTCCACGGACTACTCC
  ACTCAGATTGACATGTGGGGTGTGGGCTGCATCTTCTATGAGATGGCCACAGGCCGTCCC
  CTCTTTCCGGGCTCCACGGTGGAGGAACAGCTACACTTCATCTTCCGTATCTTAGGAACC
  CCAACTGAGGAGACGTGGCCAGGCATCCTGTCCAACGAGGAGTTCAAGACATACAACTAC
  CCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCACCCCGACTTGATAGCGACGGGGCC
  GACCTCCTCACCAAGCTGTTGCAGTTTGAGGGTCGAAATCGGATCTCCGCAGAGGATGCC
  ATGAAACATCCATTCTTCCTCAGTCTGGGGGAGCGGATCCACAAACTTCCTGACACTACT
  TCCATATTTGCACTAAAGGAGATTCAGCTACAAAAGGAGGCCAGCCTTCGGTCTTCGTCG
  ATGCCTGACTCAGGCAGGCCAGCTTTCCGCGTGGTGGACACCGAGTTCTAA
  
  Clone variation with respect to NM_006201.4
  581 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_006201 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCCCCAGGCGCC
  GCCGCGCCGGCCCCGCGGCTCTGAGGTTGCTCGCGCGCCCCCGCCGATCGCCATGGATCGGATGAAGAAG
  ATCAAACGGCAGCTGTCAATGACACTCCGAGGTGGCCGAGGCATAGACAAGACCAATGGTGCCCCTGAGC
  AGATAGGCCTGGATGAGAGTGGTGGTGGTGGCGGGCAGTGACCCTGGAGAGCCCCCCACACGTGCTGCTC
  CTGGGGAACTTCGTTCTGCACGGGGCCCACTCAGCTCTGCACCAAAAATTGTGCACAAGGACTTTGAGAA
  TGGGGTCTGATGGGAAGAGTGACCAGCCTTCAGCAACTTCCTCGGTGAAGGTGCAGTCTCCGGTGGAGGG
  GGTAATGGCCCCCCATCCCCACCAAGAATCTCACTGGGGGATTCACCAAGCCTAATCCCAAAGCTGACCA
  TCGGTGCCTGAAGGGATCTGGGAAAATCGACCCATAACCCCCTTTGAAAAACCTCTCCCCCGCGCCTCGT
  GTGCCCTATGAAAAGGTTTGGAAAAGGGACCCATTTCTCGGACAGGGGAGAGCCCTGCCCCCTCAAGGAA
  CACTCTACACCTTGGGCCTGGGTATACAGGACAGAGGGCCGCGCCTCTGAGATTGCTCGACCTCACCCAT
  CTCCTATATAACGGAGACTCCCTTTTTTATTCCGACGCAGACTGCGATGGGACTCAAACACGAGTCGTCC
  TCATCGTGCATACACGAGGCCC
  
  Kinase domain sequence

  >SC323507 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TMCYKGGCACTGGGCGAGGGTACCTATGCCACCGTCTACAAAGGSAAAAGCAAGCTCACAGACAACCTTG
  TGGCACTCATGGAGATCAGACTGGAACATGAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTGTCCCT
  GCTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACATTATCCACACGGAGAAGTCCCTCACC
  CTTGTCTTTGAGTACCTGGACAAGGACCTGAAGCAGTACCTGGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_006201
• Insert Size: 1880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006201.3, NP_006192.1
• RefSeq Size: 3130 bp
• RefSeq ORF: 1491 bp
• Locus ID: 5127
• Cytogenetics: Xp11.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2009]'
  Transcript Variant: This variant (1) encodes the shortest isoform (1).