CCRK (CDK20) (NM_012119) Human Untagged Clone

CAT#: SC323513

CDK20 (untagged)-Kinase deficient mutant (K33M) of Human cell cycle related kinase (CCRK), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK20
• Synonyms: CCRK; CDCH; P42; PNQALRE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012119, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACCAGTACTGCATCCTGGGCCGCATCGGGGAGGGCGCCCACGGCATCGTCTTCAAGGCCAAGCACG
  TGGAGACTGGCGAGATAGTTGCCCTCAAGAAGGTGGCCCTAAGGCGGTTGGAGGACGGCTTCCCTAACCA
  GGCCCTGCGGGAGATTAAGGCTCTGCAGGAGATGGAGGACAATCAGTATGTGGTACAACTGAAGGCTGTG
  TTCCCACACGGTGGAGGCTTTGTGCTGGCCTTTGAGTTCATGCTGTCGGATCTGGCCGAGGTGGTGCGCC
  ATGCCCAGAGGCCACTAGCCCAGGCACAGGTCAAGAGCTACCTGCAGATGCTGCTCAAGGGTGTCGCCTT
  CTGCCATGCCAACAACATTGTACATCGGGACCTGAAACCTGCCAACCTGCTCATCAGCGCCTCAGGCCAG
  CTCAAGATAGCGGACTTTGGCCTGGCTCGAGTCTTTTCCCCAGACGGCAGCCGCCTCTACACACACCAGG
  TGGCCACCAGGTCTGTGGGCTGCATCATGGGGGAGCTGTTGAATGGGTCCCCCCTTTTCCCGGGCAAGAA
  CGATATTGAACAGCTTTGCTATGTGCTTCGCATCTTGGGCACCCCAAACCCTCAAGTCTGGCCGGAGCTC
  ACTGAGCTGCCGGACTACAACAAGATCTCCTTTAAGGAGCAGGTGCCCATGCCCCTGGAGGAGGTGCTGC
  CTGACGTCTCTCCCCAGGCATTGGATCTGCTGGGTCAATTCCTTCTCTACCCTCCTCACCAGCGCATCGC
  AGCTTCCAAGGCTCTCCTCCATCAGTACTTCTTCACAGCTCCCCTGCCTGCCCATCCATCTGAGCTGCCG
  ATTCCTCAGCGTCTAGGGGGACCTGCCCCCAAGGCCCATCCAGGGCCCCCCCACATCCATGACTTCCACG
  TGGACCGGCCTCTTGAGGAGTCGCTGTTGAACCCAGAGCTGATTCGGCCCTTCATCCTGGAGGGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_012119 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGCTGCTGCGTG
  ACAGCGGAGGGCTAGGAAAAGGCGCAGTGGGGCCCGGAGCTGTCACCCCTGACTCGACGCAGCTTCCGTT
  CTCCTGGTGACGTGGCCTACAGGAACCGCCCCAGTGGTCAGCTGCCGCGCTGTTGCTAGGCAACAGCGTG
  CGAGCTCAGATCAGCGTGGGGTGGAGGAGAAGTGGAGTTTGGAAGTTCAGGGGCACAGGGGCACAGGCCC
  ACGACTGCAGCGGGATGGACCCAGTACTGCATCCCTGGGGCCCGCATCGGGGGAGGGCCCCCACGGCATC
  GTCTTCAAGGCCAACCACTTGGAAGACTGGGCGAGATAGTTGCCCTTCATGAAAGGTGGCCCTAAGGCGG
  TGGAAGGACGGGCTTCCCAACCAAGGCCCGGCGGGAAAATTAAGGCTTTGCAGGAAAGGGAGGACAACTC
  GATTGTGGTAAACTAAGGGTGTGTGTTCCCACCCGGTGGAGGCTTTGCCTGCCCTTTAGATTTCAGTCCT
  GTCGAATCTGGCCAGATGTGGTCGCGCTTGCCAATCCCTATAACACAGGCAGGGCTAGAGACTCACACTA
  GATGCGCTCTAAGGGGTGTCCTTGCGACATCGCCACAATT
  
  Kinase domain sequence

  >SC323513 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CMTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGCTGCTGCGTGACAGCG
  GAGGGCTAGGAAAAGGCGCAGTGGGGCCCGGAGCTGTCACCCCTGACTCGACGCAGCTTCCGTTCTCCTG
  GTGACGTGGCCTACAGGAACCGCCCCAGTGGTCAGCTGCCGCGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_012119
• ORF Size: 978 bp
• Insert Size: 1850
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_012119.2, NP_036251.2
• RefSeq Size: 2250
• RefSeq ORF: 978
• Locus ID: 23552
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene contains a kinase domain most closely related to the cyclin-dependent protein kinases. The encoded kinase may activate cyclin-dependent kinase 2 and is involved in cell growth. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Dec 2009]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.