AMPK alpha 2 (PRKAA2) (NM_006252) Human Untagged Clone

CAT#: SC323523

PRKAA2 (untagged)-Kinase deficient mutant (K45M) of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKAA2
• Synonyms: AMPK; AMPK2; AMPKa2; PRKAA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323523 sequence for NM_006252 edited (data generated by NextGen Sequencing)
  ATGGCTGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGACACTACGTGCTGGGCGAC
  ACGCTGGGCGTCGGCACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTAACAGGCCAT
  AAAGTGGCAGTTATGATCTTAAATAGACAGAAGATTCGCAGTTTAGATGTTGTTGGAAAA
  ATAAAACGAGAAATTCAAAATCTAAAACTCTTTCGTCATCCTCATATTATCAAACTATAC
  CAGGTGATCAGCACTCCAACAGATTTTTTTATGGTAATGGAATATGTGTCTGGAGGTGAA
  TTATTTGACTACATCTGTAAGCATGGACGGGTTGAAGAGATGGAAGCCAGGCGGCTCTTT
  CAGCAGATTCTGTCTGCTGTGGATTACTGTCATAGGCATATGGTTGTTCATCGAGACCTG
  AAACCAGAGAATGTCCTGTTGGATGCACACATGAATGCCAAGATAGCCGATTTCGGATTA
  TCTAATATGATGTCAGATGGTGAATTTCTGAGAACTAGTTGCGGATCTCCAAATTATGCA
  GCACCTGAAGTCATCTCAGGCAGATTGTATGCAGGTCCTGAAGTTGATATCTGGAGCTGT
  GGTGTTATCTTGTATGCTCTTCTTTGTGGCACCCTCCCATTTGATGATGAGCATGTACCT
  ACGTTATTTAAGAAGATCCGAGGGGGTGTCTTTTATATCCCAGAATATCTCAATCGTTCT
  GTCGCCACTCTCCTGATGCATATGCTGCAGGTTGACCCACTGAAACGAGCAACTATCAAA
  GACATAAGAGAGCATGAATGGTTTAAACAAGATTTGCCCAGTTACTTATTTCCTGAAGAC
  CCTTCCTATGATGCTAACGTCATTGATGATGAGGCTGTGAAAGAAGTGTGTGAAAAATTT
  GAATGTACAGAATCAGAAGTAATGAACAGTTTATATAGTGGTGACCCTCAAGACCAGCTT
  GCAGTGGCTTATCATCTTATCATTGACAATCGGAGAATAATGAACCAAGCCAGTGAGTTC
  TACCTCGCCTCTAGTCCTCCATCTGGTTCTTTTATGGATGATAGTGCCATGCATATTCCC
  CCAGGCCTGAAACCTCATCCAGAAAGGATGCCACCTCTTATAGCAGACAGCCCCAAAGCA
  AGATGTCCATTGGATGCACTGAATACGACTAAGCCCAAATCTTTAGCTGTGAAAAAAGCC
  AAGTGGCATCTTGGAATCCGAAGTCAGAGCAAACCGTATGACATTATGGCTGAAGTTTAC
  CGAGCTATGAAGCAGCTGGATTTTGAATGGAAGGTAGTGAATGCATACCATCTTCGTGTA
  AGAAGAAAAAATCCAGTGACTGGCAATTACGTGAAAATGAGCTTACAACTTTACCTGGTT
  GATAACAGGAGCTATCTTTTGGACTTTAAAAGCATTGATGATGAAGTAGTGGAGCAGAGA
  TCTGGTTCCTCAACACCTCAGCGTTCCTGTTCTGCTGCTGGCTTACACAGACCAAGATCA
  AGTTTTGATTCCACAACTGCAGAGAGCCATTCACTTTCTGGCTCTCTCACTGGCTCTTTG
  ACCGGAAGCACATTGTCTTCAGTTTCACCTCGCCTGGGCAGTCACACCATGGATTTTTTT
  GAAATGTGTGCCAGTCTGATTACTACTTTAGCCCGTTGA
  
  Clone variation with respect to NM_006252.3
  134 a=>t;135 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_006252 unedited
  ACGCCCGAACCGCAACGGGCGGAGGCGCGCACGGAGGGAGGACAACACAAGAGAGCCCGACAAGCGAACC
  GACAGAAAAACGAAAAACGACACACCAAAGGGCGGCCGCGAACACGGCACGAGGCCGCGCGCGCCGAAGA
  CGGCTGAGAAGCAGAAGCACGACGGGCGGGTGAAGACCGGACACTACGAGCTGGGCGACACGCTGGGCGT
  CGGCACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTTAACAGGCCATAAAGTGGCAGTTATGATTCT
  TAAATAGACAGAAGATTCGCAGTTTTAGATGTTTGTTGGAAAAATAAAACGAGAAAATTCAAAATCTAAA
  ACTCTTTCGTCATCCTCATATTTATCAACCTATTACCAGGGTGGATCCAGCACTTCCAACAAGATATTTT
  TTTATTGGTTATGGGGAATATGTTGGTTCGGAGAGGTGGAAATATATTGGAACTACATCTCTGTAAGGCC
  TGGGACACGGTTTTGAAAGAAAATGGGAACCAAGCCCGGCTTTTTTCGGAGAGATTCGGTTCGCGGTGGG
  GATTTACGGGCATTAGAGCATATATGGGTTGGTTACTTCAGAACACTTGAAACCCAAAAAATGTCCTTTT
  GGAAACGCACATGAAAGTGCCAGAAATGCCAGTTTTCAGTTTATCAAATTATAGTGAGAAAGGGAAATTT
  TGAGAAACCGTTGTCGAGATTCTCCAATTTGGGCCACCTGTGAATATCTTCTGCGGCAATGTGTGCCCGT
  GCTCCAAATTGATATATTGGGGGAGCGGGTGGGTATCATGTGTGACTCCCTCTCTCGTGGGGGCCCTCCC
  TCACTTTGATGGATGAAGCACGTGTGCTACCGTCATTTTATGAAGATCCACCGGGTGTCTTTCTTATATC
  TCCGCAAATCCCATCGTCTGTCGCACTCTCTCGAGCATATACTGCAGTTGTCCACTGAGCAGCGACCATC
  TTAGACTATAGACGCATGTGTAACAGATGCGGTACTATTCGAGAACATCCTCGAGGCTAGCG
  
  Kinase domain sequence

  >SC323523 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYTTGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCGCGCGCCGAAGATGGC
  TGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGACACTACGTGCTGGGCGACACGCTGGGCGTCGGC
  ACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTAACAGGCCAT
  
• Restriction Sites: Please inquire
• ACCN: NM_006252
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006252.2, NP_006243.2
• RefSeq Size: 2435 bp
• RefSeq ORF: 1659 bp
• Locus ID: 5563
• Cytogenetics: 1p32.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy
• Gene Summary: 'The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]'