TNK1 (NM_003985) Human Untagged Clone

CAT#: SC323530

TNK1 (untagged)-Kinase deficient mutant (K148M) of Human tyrosine kinase, non-receptor, 1 (TNK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNK1
• Synonyms: MGC46193
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323530 sequence for NM_003985 edited (data generated by NextGen Sequencing)
  ATGCTTCCTGAGGCTGGCTCTCTGTGGCTACTGAAGCTGCTCCGGGACATCCAGTTGGCC
  CAGTTTTACTGGCCCATCCTTGAGGAGCTTAATGTCACTCGGCCAGAGCACTTCGACTTT
  GTAAAGCCTGAGGACCTGGACGGCATTGGCATGGGCCGGCCTGCCCAGCGCAGACTGTCC
  GAAGCTCTGAAAAGGCTACGTTCTGGGCCTAAGTCTAAGAACTGGGTCTACAAGATCCTT
  GGAGGTTTTGCCCCTGAGCACAAGGAGCCCACCCTGCCCTCGGACAGCCCACGGCACCTC
  CCTGAGCCAGAGGGGGGCCTCAAGTGTCTGATCCCAGAGGGTGCTGTTTGCAGAGGGGAG
  CTGCTGGGTTCAGGCTGCTTCGGTGTGGTGCACCGAGGGCTGTGGACGCTGCCCAGTGGC
  AAGAGTGTCCCAGTGGCTGTCATGTCCCTCCGGGTAGGTCCCGAAGGCCCGATGGGCACA
  GAACTGGGGGACTTCCTGCGAGAGGTATCGGTCATGATGAACTTGGAGCACCCACACGTG
  CTGCGTCTGCACGGCCTTGTACTGGGCCAGCCTCTGCAGATGGTGATGGAGCTGGCGCCA
  CTGGGCTCCCTGCACGCGCGCCTAACGGCCCCGGCCCCGACACCCCCGCTGCTCGTGGCC
  CTGCTCTGCCTCTTCCTGCGGCAGCTGGCGGGAGCCATGGCGTACCTGGGGGCCCGCGGG
  CTGGTGCACCGAGACCTCGCTACGCGCAACCTACTGCTGGCGTCGCCGCGCACCATCAAG
  GTGGCTGACTTCGGGCTGGTGCGGCCTCTGGGCGGTGCCCGGGGCCGCTACGTCATGGGC
  GGGCCCCGCCCTATCCCCTACGCCTGGTGTGCCCCAGAGAGCCTGCGCCACGGAGCCTTC
  TCGTCTGCCTCGGACGTGTGGATGTTTGGGGTGACGCTGTGGGAGATGTTCTCCGGGGGC
  GAGGAACCCTGGGCCGGGGTCCCACCGTACCTCATCCTGCAGCGGCTGGAGGACAGAGCC
  CGGCTGCCTAGGCCTCCCCTCTGCTCCAGGGCCCTCTACTCCCTCGCCTTGCGCTGCTGG
  GCCCCCCACCCTGCCGACCGGCCTAGCTTTTCCCACCTGGAGGGGCTGCTGCAAGAGGCC
  GGGCCTTCGGAAGCATGTTGTGTGAGGGATGTCACAGAACCAGGCGCCCTGAGGATGGAG
  ACTGGTGACCCCATCACAGTCATCGAGGGCAGCCCCGACTCCACAATCTGGAAGGGCCAG
  AATGGTCGCACCTTCAAAGTGGGCAGCTTCCCAGCCTCGGCAGTGACGCTGGCAGATGCG
  GGGGGCTTGCCAGCCACCCGTCCAGTCCACAGAGGCACCCCTGCCCGGGGAGATCAACAC
  CCAGGAAGCATAGATGGAGACAGAAAGAAGGCAAATCTTTGGGATGCGCCCCCAGCACGG
  GGCCAGAGGAGGAACATGCCCCTGGAGAGGATGAAAGGCATTTCCAGGAGTCTGGAGTCA
  GTTCTGTCCCTCGGTCCTCGTCCCACAGGGGGTGGTTCAAGCCCCCCTGAAATTCGACAA
  GCCAGAGCTGTGCCCCAGGGACCTCCAGGCCTGCCTCCACGCCCACCTTTATCCTCTAGC
  TCTCCTCAGCCCAGCCAGCCCTCTAGGGAGAGGCTTCCCTGGCCCAAAAGAAAACCCCCA
  CACAATCACCCCATGGGAATGCCTGGAGCCCGTAAAGCCGCTGCCCTCTCTGGAGGCCTC
  TTGTCCGATCCTGAGTTGCAGAGGAAGATTATGGAGATGGAGCTGAGTGTGCATGGGGTC
  ACCCACCAGGAGTGCCAGACAGCACTAGGAGCCACTGGGGGAGATGTGGTTTCTGCCATC
  CGGAACCTCAAGGTAGATCAGCTCTTCCACCTGAGTAGCCGGTCCAGAGCTGACTGCTGG
  CGCATCCTGGAGCATTACCAGTGGGACCTCTCAGCTGCCAGCCGCTATGTCCTGGCCAGG
  CCCTGA
  
  Clone variation with respect to NM_003985.3
  21 c=>t;443 a=>t;1777 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003985 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGACCAGGTGGA
  GCTGGAGACCTGGTCTCTCTAGGGCCTACCCTGAGCTCACCATCTGAAGGAGAGTGCCATCATCCTTAGG
  AACTCCTTCTCCAGACATGCTTCCTGAGGCTGGCTCTCTGTGGCTACTGAAGCTGCTCCGGGACATCCAG
  TTGGCCCAGTTTTACTGGCCCATCCTTGAGGAGCTTAATGTCACTCGGCCAGAGCACTTCGACTTTGTAA
  AGCCTGAGGACCTGGACGGCATTGGCATGGGCCGGCCTGCCCAGCGCAGACTGTCCGAAGCTCTGAAAAG
  GCTACGTTCTGGGCCTAAGTCTAAGAACTGGGTCTACAAGATCCTTGGAGGTTTTGCCCCTGAGCACAAG
  AGCCCACCCTGCCCTCGGACAGCCCACGGCACCTCCCTGAGCCAGAGGGGGCCTCAAGTGTCTGATCCCA
  GAGGGTGCTGTTTGCAGAGGGAGCTGCTGGGTTCAGCTGCTTCGTGTGTGCACCGAGGGCTGTGACGCTG
  CCCAGTGCAGAGTGTCCAGTGGCTGTCATGTCCCTCCGGGTAGTCCCGAGCCCGATGGCAACAGACTGGG
  GGACTTCTGCGGAAGGTTTCGTCATGATGAACTTGGAGCCACCCCACACTGCTGCGTCTGCACGCCTTTT
  ACTGGCCAGCCTTTGCCAATGGTGATGGACTGGCGCCATGGCTCCCTGCCGCGCGCTACGCCCGCCGGAA
  CCCCCGGTGTCTTGCCTGCTTGCTTTTCTGCGACTGGGGAACCATGGCTACCTGGACAAGCCTGTACGAC
  CTTGTACCGCACCATGTGTGGTTGCCGGCGCACACATAAGGTGGCGTAATCTTCG
  
  Kinase domain sequence

  >SC323530 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GMGKCTGGATMTGCTGCTTCGGTGTGGTGCACCGAGGGCTGTGGACGCTGCCCAGTGGCAAGAGTGTCCC
  AGTGGCTGTCATGTCCCTCCGGGTAGGTCCCGAAGGCCCGATGGGCACAGAACTGGGGGACTTCCTGCGA
  GAGGTATCGGTCATGATGAACTTGGAGCACCCACACGTGCTGCGTCTGCACGGCCTTGTACTGGGCCAGC
  CTCTGCAGATGGTGATGGAGCTGGCGCCACTGGGCTCCCTGCACG
  
• Restriction Sites: Please inquire
• ACCN: NM_003985
• ORF Size: 2001 bp
• Insert Size: 2850
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003985.1, NP_003976.1
• RefSeq Size: 2771
• RefSeq ORF: 2001
• Locus ID: 8711
• Domains: pkinase, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene belongs to the tyrosine protein kinase family. Tyrosine protein kinases are important regulators of intracellular signal transduction pathways, mediating cellular proliferation, survival, and development. This gene is highly expressed in fetal tissues and at lower levels in few adult tissues, thus may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. It plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in a shorter isoform (2) missing a 5 aa protein segment compared to isoform 1.