TAOK1 (NM_020791) Human Untagged Clone

CAT#: SC323537

TAOK1 (untagged)-Kinase deficient mutant (K57M) of Human TAO kinase 1 (TAOK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAOK1
• Synonyms: hKFC-B; hTAOK1; KFC-B; MAP3K16; MARKK; PSK-2; PSK2; TAO1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323537 sequence for NM_020791 edited (data generated by NextGen Sequencing)
  ATGCCATCAACTAACAGAGCAGGCAGCCTGAAGGACCCTGAAATTGCAGAGCTCTTCTTC
  AAAGAAGATCCAGAGAAGCTCTTCACAGATCTCAGAGAAATTGGCCATGGAAGCTTTGGA
  GCAGTGTATTTTGCACGAGATGTGCGTACCAATGAAGTGGTGGCCATCATGAAAATGTCT
  TATAGTGGAAAGCAGTCTACTGAGAAATGGCAGGATATTATTAAGGAAGTCAAGTTTCTA
  CAAAGAATAAAACATCCCAACAGTATAGAATACAAAGGCTGTTATTTACGTGAACACACA
  GCATGGCTTGTAATGGAATATTGTTTAGGATCTGCTTCGGATTTACTAGAAGTTCACAAA
  AAGCCATTACAAGAAGTGGAAATAGCAGCAATTACACATGGTGCTCTTCAGGGATTAGCC
  TACTTACATTCTCATACTATGATTCATAGAGATATCAAAGCAGGAAATATCCTTCTGACA
  GAACCAGGCCAGGTGAAACTTGCTGACTTTGGCTCTGCTTCCATGGCATCACCTGCCAAT
  TCCTTTGTGGGAACGCCGTATTGGATGGCCCCAGAAGTAATTTTAGCCATGGATGAAGGA
  CAATATGATGGCAAAGTAGATGTGTGGTCTCTTGGAATAACATGTATTGAACTAGCGGAA
  AGGAAGCCTCCTTTATTTAATATGAATGCAATGAGTGCATTATATCACATAGCCCAAAAT
  GAATCCCCTACACTACAGTCTAATGAATGGTCTGATTATTTTCGCAACTTTGTAGATTCT
  TGCCTCCAGAAAATCCCTCAAGATCGACCTACATCAGAGGAACTTTTAAAGCACATATTT
  GTTCTTCGGGAGCGCCCTGAAACCGTGTTAATAGATCTCATTCAGAGGACAAAGGATGCA
  GTAAGAGAGCTGGACAATCTGCAGTATCGAAAGATGAAGAAACTCCTTTTCCAGGAGGCA
  CATAATGGACCAGCAGTAGAAGCACAGGAAGAAGAAGAGGAACAAGATCATGGTGTTGGC
  CGGACAGGAACAGTTAATAGTGTTGGAAGTAATCAATCCATTCCCAGCATGTCCATCAGT
  GCCAGCAGCCAAAGCAGTAGTGTTAACAGTCTTCCAGATGTCTCAGATGACAAGAGTGAG
  CTAGACATGATGGAGGGAGACCACACAGTGATGTCTAACAGTTCTGTTATCCATTTAAAA
  CCAGAGGAAGAAAATTACAGAGAAGAGGGAGATCCTAGAACAAGAGCATCAGATCCACAA
  TCTCCACCCCAAGTATCTAGTCACAAATCACACTATCGTAATCGAGAACACTTTGCTACT
  ATACGGACAGCATCACTGGTTACGAGGCAAATGCAAGAACATGAGCAGGACTCTGAGCTT
  AGAGAACAAATGTCTGGCTATAAGCGAATGAGGCGACAACATCAAAAGCAACTGATGACT
  CTGGAAAACAAGCTAAAGGCTGAGATGGATGAACATCGCCTCAGATTAGACAAAGATCTT
  GAAACTCAGCGTAACAATTTTGCTGCAGAAATGGAGAAACTTATCAAGAAACACCAGGCT
  GCCATGGAGAAAGAGGCTAAAGTGATGTCCAATGAAGAGAAAAAATTTCAGCAACATATT
  CAGGCCCAACAGAAGAAAGAACTGAATAGTTTTCTCGAGTCCCAGAAAAGAGAGTATAAA
  CTTCGAAAAGAGCAGCTTAAAGAGGAGCTAAATGAAAACCAGAGTACCCCCAAAAAAGAA
  AAACAGGAGTGGCTTTCAAAGCAGAAGGAGAATATACAGCATTTCCAAGCAGAAGAAGAA
  GCTAACCTTCTTCGACGTCAAAGACAATACCTAGAGCTGGAATGCCGTCGCTTCAAGAGA
  AGAATGTTACTTGGGCGTCATAACTTAGAGCAGGACCTTGTCAGGGAGGAGTTAAACAAA
  AGACAGACTCAGAAGGACTTAGAGCATGCCATGCTACTCCGACAGCATGAATCTATGCAA
  GAACTGGAGTTCCGCCACCTCAACACAATTCAGAAGATGCGCTGTGAGTTGATCAGATTA
  CAGCATCAAACTGAGCTCACTAACCAGCTGGAATATAATAAGCGAAGAGAACGAGAACTA
  AGACGAAAGCATGTCATGGAAGTTCGACAACAGCCTAAGAGTTTGAAGTCTAAAGAACTC
  CAAATAAAAAAGCAGTTTCAGGATACCTGCAAAATCCAAACCAGACAGTACAAAGCATTA
  AGAAATCACCTGCTGGAGACTACACCAAAGAGTGAGCACAAAGCTGTTCTGAAACGGCTC
  AAGGAGGAACAGACCCGGAAATTAGCTATCTTGGCTGAGCAGTATGATCACAGCATTAAT
  GAAATGCTCTCCACACAAGCCCTGCGTTTGGATGAAGCACAGGAAGCAGAGTGCCAGGTT
  TTGAAGATGCAGCTGCAGCAGGAACTGGAGCTGTTGAATGCGTATCAGAGCAAAATCAAG
  ATGCAAGCTGAGGCACAACATGATCGAGAGCTTCGCGAGCTTGAACAGAGGGTCTCCCTC
  CGGAGGGCACTCTTAGAACAAAAGATTGAAGAAGAGATGTTGGCTTTGCAGAATGAGCGC
  ACAGAACGAATACGAAGCCTGTTGGAACGTCAAGCCAGAGAGATTGAAGCTTTTGACTCT
  GAAAGCATGAGACTAGGTTTTAGTAATATGGTCCTTTCTAATCTCTCCCCTGAGGCATTC
  AGCCACAGCTACCCGGGAGCTTCTGGTTGGTCACACAACCCTACTGGGGGTCCAGGACCT
  CACTGGGGTCATCCCATGGGTGGCCCACCACAAGCTTGGGGCCATCCAATGCAAGGTGGA
  CCCCAGCCATGGGGTCACCCTTCAGGGCCAATGCAAGGGGTACCTCGAGGTAGCAGTATG
  GGAGTCCGCAATAGCCCCCAGGCTCTGAGGCGGACAGCTTCTGGGGGACGGACGGAGCAG
  GGCATGAGCAGAAGCACGAGTGTCACTTCACAAATATCCAATGGGTCACACATGTCTTAT
  ACATAA
  
  Clone variation with respect to NM_020791.2
  170 a=>t;699 c=>a;1279 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_020791 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCAACGTGACTTCA
  TTCATACAGATGAACCAAGGAACGGGATAGCAGTATAAAATTAGAATCAAGACAGCTGACTGCTCAGCAG
  GATGCCATCAACTAACAGAGCAGGCAGCCTGAAGGACCCTGAAATTGCAGAGCTCTTCTTCAAAGAAGAT
  CCAGAGAAGCTCTTCACAGATCTCAGAGAAAATTGGCCATGGAAGCTTTTGGAGCAGTGTATTTTTGCAC
  GAGATGTGCGTACCAATGAAGTGGTTGGCCATCATGAAAAAGGTCTTATAGTGGAAAGCAGTCTACTGGA
  GAAAATGCCAGGAAATTATTTAAGGAGGTCCAAGTTTTCTACAAGAATTAAAACCATCCCAAACGGTATA
  GAAAAAACAAAGGCTGGTTTATTTACTTGACCACAGGAATGCCTGGTATTGAAAATGGTTAAGAATCGGC
  TTCGAATTTCCAGAATTTACAAAAAGCCCTTTACAGGAGTGGGAATAGCGACATTTACCTGGGGGCTTCT
  CGGGTAATACCAACTCACTCTCTACCTGAGATTCAGAGATATTCAGCCGGAATTCCTCTGAGAAACCGCG
  CGTGTGACTGTGCGCTTGGCTCGTCTCGGGACACTGCGCATTCTTGGGAACCCTATGATAGGCCCGAGTA
  TTTTGCCTGTGTAGAGCAATATGTCGATAAGTGTGCCTTGGAAACCGATGACACCGAGAGACTCCTATTA
  TAATGCTGAGTCATATGCATGCGATAGTCTCACGCTTAAGGTCGGATTGCACCTGGTATCTGCC
  
  Kinase domain sequence

  >SC323537 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGAGWCAGAGAGCTCTTCACAGATCTCAGAGAAATTGGCCATGGAAGCTTTGGAGCAGTGTATTTTGCAC
  GAGATGTGCGTACCAATGAAGTGGTGGCCATCATGAAAATGTCTTATAGTGGAAAGCAGTCTACTGAGAA
  ATGGCAGGATATTATTAAGGAAGTCAAGTTTCTACAAAGAATAAAACATCCCAACAGTATAGAATACAAA
  GGCTGTTATTTACGTGAACACACAGCATGGCTTGTAATGGAATAT
  
• Restriction Sites: Please inquire
• ACCN: NM_020791
• ORF Size: 3006 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_020791.1, NP_065842.1
• RefSeq Size: 4662
• RefSeq ORF: 3006
• Locus ID: 57551
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway