Mps1 (TTK) (NM_003318) Human Untagged Clone

CAT#: SC323540

TTK (untagged)-Kinase deficient mutant (K553M) of Human TTK protein kinase (TTK)


  "NM_003318" in other vectors (8)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TTK
Synonyms CT96; ESK; MPH1; MPS1; MPS1L1; PYT
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323540 sequence for NM_003318 edited (data generated by NextGen Sequencing)
ATGGAATCCGAGGATTTAAGTGGCAGAGAATTGACAATTGATTCCATAATGAACAAAGTG
AGAGACATTAAAAATAAGTTTAAAAATGAAGACCTTACTGATGAACTAAGCTTGAATAAA
ATTTCTGCTGATACTACAGATAACTCGGGAACTGTTAACCAAATTATGATGATGGCAAAC
AACCCAGAGGACTGGTTGAGTTTGTTGCTCAAACTAGAGAAAAACAGTGTTCCGCTAAGT
GATGCTCTTTTAAATAAATTGATTGGTCGTTACAGTCAAGCAATTGAAGCGCTTCCCCCA
GATAAATATGGCCAAAATGAGAGTTTTGCTAGAATTCAAGTGAGATTTGCTGAATTAAAA
GCTATTCAAGAGCCAGATGATGCACGTGACTACTTTCAAATGGCCAGAGCAAACTGCAAG
AAATTTGCTTTTGTTCATATATCTTTTGCACAATTTGAACTGTCACAAGGTAATGTCAAA
AAAAGTAAACAACTTCTTCAAAAAGCTGTAGAACGTGGAGCAGTACCACTAGAAATGCTG
GAAATTGCCCTGCGGAATTTAAACCTCCAAAAAAAGCAGCTGCTTTCAGAGGAGGAAAAG
AAGAATTTATCAGCATCTACGGTATTAACTGCCCAAGAATCATTTTCCGGTTCACTTGGG
CATTTACAGAATAGGAACAACAGTTGTGATTCCAGAGGACAGACTACTAAAGCCAGGTTT
TTATATGGAGAGAACATGCCACCACAAGATGCAGAAATAGGTTACCGGAATTCATTGAGA
CAAACTAACAAAACTAAACAGTCATGCCCATTTGGAAGAGTCCCAGTTAACCTTCTAAAT
AGCCCAGATTGTGATGTGAAGACAGATGATTCAGTTGTACCTTGTTTTATGAAAAGACAA
ACCTCTAGATCAGAATGCCGAGATTTGGTTGTGCCTGGATCTAAACCAAGTGGAAATGAT
TCCTGTGAATTAAGAAATTTAAAGTCTGTTCAAAATAGTCATTTCAAGGAACCTCTGGTG
TCAGATGAAAAGAGTTCTGAACTTATTATTACTGATTCAATAACCCTGAAGAATAAAACG
GAATCAAGTCTTCTAGCTAAATTAGAAGAAACTAAAGAGTATCAAGAACCAGAGGTTCCA
GAGAGTAACCAGAAACAGTGGCAATCTAAGAGAAAGTCAGAGTGTATTAACCAGAATCCT
GCTGCATCTTCAAATCACTGGCAGATTCCGGAGTTAGCCCGAAAAGTTAATACAGAGCAG
AAACATACCACTTTTGAGCAACCTGTCTTTTCAGTTTCAAAACAGTCACCACCAATATCA
ACATCTAAATGGTTTGACCCAAAATCTATTTGTAAGACACCAAGCAGCAATACCTTGGAT
GATTACATGAGCTGTTTTAGAACTCCAGTTGTAAAGAATGACTTTCCACCTGCTTGTCAG
TTGTCAACACCTTATGGCCAACCTGCCTGTTTCCAGCAGCAACAGCATCAAATACTTGCC
ACTCCACTTCAAAATTTACAGGTTTTAGCATCTTCTTCAGCAAATGAATGCATTTCGGTT
AAAGGAAGAATTTATTCCATATTAAAGCAGATAGGAAGTGGAGGTTCAAGCAAGGTATTT
CAGGTGTTAAATGAAAAGAAACAGATATATGCTATAAAATATGTGAACTTAGAAGAAGCA
GATAACCAAACTCTTGATAGTTACCGGAACGAAATAGCTTATTTGAATAAACTACAACAA
CACAGTGATAAGATCATCCGACTTTATGATTATGAAATCACGGACCAGTACATCTACATG
GTAATGGAGTGTGGAAATATTGATCTTAATAGTTGGCTTAAAAAGAAAAAATCCATTGAT
CCATGGGAACGCAAGAGTTACTGGAAAAATATGTTAGAGGCAGTTCACACAATCCATCAA
CATGGCATTGTTCACAGTGATCTTAAACCAGCTAACTTTCTGATAGTTGATGGAATGCTA
AAGCTAATTGATTTTGGGATTGCAAACCAAATGCAACCAGATACAACAAGTGTTGTTAAA
GATTCTCAGGTTGGCACAGTTAATTATATGCCACCAGAAGCAATCAAAGATATGTCTTCC
TCCAGAGAGAATGGGAAATCTAAGTCAAAGATAAGCCCCAAAAGTGATGTTTGGTCCTTA
GGATGTATTTTGTACTATATGACTTACGGGAAAACACCATTTCAGCAGATAATTAATCAG
ATTTCTAAATTACATGCCATAATTGATCCTAATCATGAAATTGAATTTCCCGATATTCCA
GAGAAAGATCTTCAAGATGTGTTAAAGTGTTGTTTAAAAAGGGACCCAAAACAGAGGATA
TCCATTCCTGAGCTCCTGGCTCATCCATATGTTCAAATTCAAACTCATCCAGTTAACCAA
ATGGCCAAGGGAACCACTGAAGAAATGAAATATGTTCTGGGCCAACTTGTTGGTCTGAAT
TCTCCTAACTCCATTTTGAAAGCTGCTAAAACTTTATATGAACACTATAGTGGTGGTGAA
AGTCATAATTCTTCATCCTCCAAGACTTTTGAAAAAAAAAGGGGAAAAAAATGA

Clone variation with respect to NM_003318.4
>OriGene 5' read for mutant NM_003318 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGATAGCGACGGGGA
AATTCAAACGTGTTTGCGGAAAGGAGTTTGGGTTCCATCTTTTCATTTCCCCAGCGCAGCTTTCTGTAGT
TTTTTTCTTAGAAATGGAATCCGAGGATTTAAGTGGCAGAGAATTGACAATTGATTCCATAATGAACAAA
GTGAGAGACATTAAAAATAAGTTTAAAAATGAAGACCTTACTGATGAACTAAGCTTGAATAAAATTTCTG
CTGATACTACAGATAACTCGGGAACTGTTAACCAAATTATGATGATGGCAAACAACCCAGAGGACTGTTG
AGTTTGTTGCCTAAACTAAAAAAAACGTTGTTCCGCTAGTGATGCTTCTTTTAATAAATTGATGGTCGTA
CAGTCAAGCATTGAACCGCTCCCCCGATAATTTGGCCAAATGAAATTTTGCTGATTACATTGAATTGCCT
GATTAAACCATCCAGAACCCGATATATGACTTGCACTCTTTCAATGCCCAGCCAACTGGAGAATTTGGCT
TTGTTATATCCTGGCAATTACTCGCCAAGTGATGTCAAAAGAGTACACTCTCTCAAGCCTTAAACGTGCA
CATACCAAAGTGGGAATGCCGCTGGATATAACTCAAACACGTCTTCAAAAGAAAAAAAAATAACACTCCG
GTATGCGCAGACTTCGGATATGGGTCTCAGAGGAACTGTATCAGGACCATCACGGTTATAGGAACGCCCA
CTGACTATTTCGTCA
>SC323540 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TMTATTAAAGCAGATAGGAKGGAGGTTCAGCAAGGTATTTCAGGTGTTAAATGAAAAGAAACAGATATAT
GCTATAATGTATGTGAACTTAGAAGAAGCAGATAACCAAACTCTTGATAGTTACCGGAACGAAATAGCTT
ATTTGAATAAACTACAACAACACAGTGATAAGATCATCCGACTTTATGATTATGAAATCACGGACCAGTA
CATCTACATGGTAATGGAGTGTGGAAATATTGATCTTAATAGTTG
Restriction Sites Please inquire     
ACCN NM_003318
Insert Size 2900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003318.3, NP_003309.2
RefSeq Size 2984 bp
RefSeq ORF 2574 bp
Locus ID 7272
Cytogenetics 6q14.1
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway
Gene Summary 'This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]'
Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).

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