PINK1 (NM_032409) Human Untagged Clone

CAT#: SC323548

PINK1 (untagged)-Kinase deficient mutant (K219M) of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein


  "NM_032409" in other vectors (7)

Reconstitution Protocol

USD 980.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "PINK1"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PINK1
Synonyms BRPK; PARK6
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323548 sequence for NM_032409 edited (data generated by NextGen Sequencing)
ATGGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCTGGGTCGAGCGCTGCTGCTGCGC
TTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGCGGCCGGGCCCGGCGGCGGGCTGT
GTCCGCGGGGAGCGTCCAGGCTGGGCCGCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGG
CTCGGGCTCCCTAACCGTCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGCGG
TTGCAGCGGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGCGGCCGGGCAGTC
TTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGAAAAACAGGCGGAGAGCCGGCGG
GCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGGGGCCT
GACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAG
TCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAG
AACCTGGAGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCA
CCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCATGATG
ATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCCAGGAG
CTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCAGTCACTTACAGAAAA
TCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCC
TTCACCTCTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCC
TCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAAC
TATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCGCCGCC
ATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAACAGGGCATCGCGCAC
AGAGACCTGAAATCCGACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTG
GTGATCGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTC
AGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACG
GCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAGTGGGA
GCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCAC
CTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCA
GACGTGAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCC
CGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAG
AATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTTTGTTG
GCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCT
AACCTGGAGTGTGAAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCC
CTGTGA

Clone variation with respect to NM_032409.2
656 a=>t
>OriGene 5' read for mutant NM_032409 unedited
CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCGGTGGTGGCG
GCAGCGGCGGCTGCGGGGGCACCGGGCCGCGGCGCCACCATGGCGGTGCGACAGGCGCTGGGCCGCGGCC
TGCAGCTGGGTCGAGCGCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGCGGCC
GGGCCCGGCGGCGGGCTGTGTCCCGCGGGGGAGCGTCCAGGCTGGGCCGCAAGGAACCGGGCGGCGGGAG
CCTTCCGCAAGGGTCCGGGCTCTCGGCTTTCCCTACCCTGCTCTCCGCTTTCTTTCCGCCAATCTCGTGT
GGCCGGCCTGGGCGGGCGCCGGGTTGCAACCGCCCGTTTCTTGGTGGGCGGGCCTTGGGGGCTGGCGCGG
GCCCCTGGGCGGCGGGGCATTTTTTTTGGGCCCTTCGGGCCTAGGGGTGGGGCCCCATTCGGGGAAAAAA
CAGGGCGGAAAGCCCGGGGGGCGGGCCCGGGCCGTGGCAGGGAATTCCACCCATTTTTATACCGAAAAAA
CCACCCGGGGCCAAACCCTTGAAACCAAAACCTTTTGGAGGGTTCTGGGGGGAAGGATTAGTTAGCGGGT
CTCCAATGAAGGCGCCTCACATAACTAGGGAAACCACATCGCGAATC
>SC323548 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TGRGTGMAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCWGWAG
CGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCATGATGATGTGGAACATCTCGGCAGGTTCCTCCA
GCGAAGCCATCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTA
TGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACT
Restriction Sites Please inquire     
ACCN NM_032409
ORF Size 1746 bp
Insert Size 2710
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Reference Data
RefSeq NM_032409.1, NP_115785.1
RefSeq Size 2700
RefSeq ORF 1746
Locus ID 65018
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Parkinson's disease
Gene Summary This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.