LATS2 (NM_014572) Human Untagged Clone

CAT#: SC323550

LATS2 (untagged)-Kinase deficient mutant (K697M) of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LATS2
• Synonyms: KPM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323550 sequence for NM_014572 edited (data generated by NextGen Sequencing)
  ATGAGGCCAAAGACTTTTCCTGCCACGACTTATTCTGGAAATAGCCGGCAGCGACTGCAA
  GAGATTCGTGAGGGGTTAAAACAGCCATCCAAGTCTTCGGTTCAGGGGCTACCCGCAGGA
  CCAAACAGTGACACTTCCCTGGATGCCAAAGTCCTGGGGAGCAAAGATGCCACCAGGCAG
  CAGCAGCAGATGAGAGCCACCCCAAAGTTCGGACCTTATCAGAAAGCCTTGAGGGAAATC
  AGATATTCCTTGTTGCCTTTTGCTAATGAATCGGGCACCTCTGCAGCTGCAGAAGTGAAC
  CGGCAAATGCTGCAGGAACTGGTGAACGCAGGATGCGACCAGGAGATGGCTGGCCGAGCT
  CTCAAGCAGACTGGCAGCAGGAGCATCGAGGCCGCCCTGGAGTACATCAGCAAGATGGGC
  TACCTGGACCCGAGGAATGAGCAGATTGTGCGGGTCATTAAGCAGACCTCCCCAGGAAAG
  GGGCTCATGCCAACCCCAGTGACGCGGAGGCCCAGCTTCGAAGGAACCGGCGATTCGTTT
  GCGTCCTACCACCAGCTGAGCGGTACCCCCTACGAGGGCCCAAGCTTCGGCGCTGACGGC
  CCCACGGCGCTGGAGGAGATGCCGCGGCCGTACGTGGACTACCTTTTCCCCGGAGTCGGC
  CCCCACGGGCCCGGCCACCAGCACCAGCACCCACCCAAGGGCTACGGTGCCAGCGTAGAG
  GCAGCAGGGGCACACTTCCCGCTGCAGGGCGCGCACTACGGGCGGCCGCACCTGCTGGTG
  CCTGGGGAACCCCTGGGCTACGGAGTGCAGCGCAGCCCCTCCTTCCAGAGCAAGACGCCG
  CCGGAGACCGGGGGTTACGCCAGCCTGCCCACGAAGGGCCAGGGAGGACCGCCAGGCGCC
  GGCCTCGCTTTCCCACCCCCTGCCGCCGGGCTCTACGTGCCGCACCCACACCACAAGCAG
  GCCGGTCCCGCGGCCCACCAGCTGCATGTGCTGGGCTCCCGCAGCCAGGTGTTCGCCAGC
  GACAGCCCCCCGCAGAGCCTGCTCACTCCCTCGCGGAACAGCCTCAACGTGGACCTGTAT
  GAATTGGGCAGCACCTCCGTCCAGCAGTGGCCGGCTGCCACCCTGGCCCGCCGGGACTCC
  CTGCAGAAGCCGGGCCTGGAGGCGCCGCCGCGCGCGCACGTGGCCTTCCGGCCTGACTGC
  CCAGTGCCCAGCAGGACCAACTCCTTCAACAGCCACCAGCCGCGGCCCGGTCCGCCTGGC
  AAGGCCGAGCCCTCCCTGCCCGCCCCCAACACCGTGACGGCTGTCACGGCCGCGCACATC
  TTGCACCCGGTGAAGAGCGTGCGTGTGCTGAGGCCGGAGCCGCAGACGGCTGTGGGGCCC
  TCGCACCCCGCCTGGGTGCCCGCGCCTGCCCCGGCCCCCGCCCCCGCCCCCGCCCCGGCT
  GCGGAGGGCTTGGACGCCAAGGAGGAGCATGCCCTGGCGCTGGGCGGCGCAGGCGCCTTC
  CCGCTGGACGTGGAGTACGGAGGCCCAGACCGGAGGTGCCCGCCTCCGCCCTACCCGAAG
  CACCTGCTGCTGCGCAGCAAGTCGGAGCAGTACGACCTGGACAGCCTGTGCGCAGGCATG
  GAGCAGAGCCTCCGTGCGGGCCCCAACGAGCCCGAGGGCGGCGACAAGAGCCGCAAAAGC
  GCCAAGGGGGACAAAGGCGGAAAGGATAAAAAGCAGATTCAGACCTCTCCCGTTCCCGTC
  CGCAAAAACAGCAGAGACGAAGAGAAGAGAGAGTCACGCATCAAGAGCTACTCGCCATAC
  GCCTTTAAGTTCTTCATGGAGCAGCACGTGGAGAATGTCATCAAAACCTACCAGCAGAAG
  GTTAACCGGAGGCTGCAGCTGGAGCAAGAAATGGCCAAAGCTGGACTCTGTGAAGCTGAG
  CAGGAGCAGATGCGGAAGATCCTCTACCAGAAAGAGTCTAATTACAACAGGTTAAAGAGG
  GCCAAGATGGACAAGTCTATGTTTGTCAAGATCAAAACCCTGGGGATCGGTGCCTTTGGA
  GAAGTGTGCCTTGCTTGTAAGGTGGACACTCACGCCCTGTACGCCATGAAGACCCTAAGG
  AAAAAGGATGTCCTGAACCGGAATCAGGTGGCCCACGTCAAGGCCGAGAGGGACATCCTG
  GCCGAGGCAGACAATGAGTGGGTGGTCAAACTCTACTACTCCTTCCAAGACAAAGACAGC
  CTGTACTTTGTGATGGACTACATCCCTGGTGGGGACATGATGAGCCTGCTGATCCGGATG
  GAGGTCTTCCCTGAGCACCTGGCCCGGTTCTACATCGCAGAGCTGACTTTGGCCATTGAG
  AGTGTCCACAAGATGGGCTTCATCCACCGAGACATCAAGCCTGATAACATTTTGATAGAT
  CTGGATGGTCACATTAAACTCACAGATTTCGGCCTCTGCACTGGGTTCAGGTGGACTCAC
  AATTCCAAATATTACCAGAAAGGGAGCCATGTCAGACAGGACAGCATGGAGCCCAGCGAC
  CTCTGGGATGATGTGTCTAACTGTCGGTGTGGGGACAGGCTGAAGACCCTAGAGCAGAGG
  GCGCGGAAGCAGCACCAGAGGTGCCTGGCACATTCACTGGTGGGGACTCCAAACTACATC
  GCACCCGAGGTGCTCCTCCGCAAAGGGTACACTCAACTCTGTGACTGGTGGAGTGTTGGA
  GTGATTCTCTTCGAGATGCTGGTGGGGCAGCCGCCCTTTTTGGCACCTACTCCCACAGAA
  ACCCAGCTGAAGGTGATCAACTGGGAGAACACGCTCCACATTCCAGCCCAGGTGAAGCTG
  AGCCCTGAGGCCAGGGACCTCATCACCAAGCTGTGCTGCTCCGCAGACCACCGCCTGGGG
  CGGAATGGGGCCGATGACCTGAAGGCCCACCCCTTCTTCAGCGCCATTGACTTCTCCAGT
  GACATCCGGAAGCAGCCAGCCCCCTACGTTCCCACCATCAGCCACCCCATGGACACCTCG
  AATTTCGACCCCGTAGATGAAGAAAGCCCTTGGAACGATGCCAGCGAAGGTAGCACCAAG
  GCCTGGGACACACTCACCTCGCCCAATAACAAGCATCCTGAGCACGCATTTTACGAATTC
  ACCTTCCGAAGGTTCTTTGATGACAATGGCTACCCCTTTCGATGCCCAAAGCCTTCAGGA
  GCAGAAGCTTCACAGGCTGAGAGCTCAGATTTAGAAAGCTCTGATCTGGTGGATCAGACT
  GAAGGCTGCCAGCCTGTGTACGTGTAG
  
  Clone variation with respect to NM_014572.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014572 unedited
  ACGCCCGCACAGCAAAGGGCGGTAGGCGTTACGGTGGGAGGACATATAAGCAGAGCACATAAAGGCGACA
  CCATAGAATACAAGCAACTCGAACTCAAAGCAGCGGCCGCGAATACGGCACGAGGAACAAAATCAAAAGG
  AAATAATAATGAAAGAATGACTACAACAAACTGATACTATTGACAGTATTTTTTTTTTAAATAATAAAAC
  ACTTTTAAGAAGATTGTATTTATGGTAAAAGGAAACTGGACTAACAATGAGGCCAAAGACTATTCCTGCC
  ACGACTTATTCTGGAAATAGCCGGCAGCGACTGCAAGAGATTCGTGAGGGGTTAAAGCAGCCATCCAAGA
  CTTCGGTTCAGGGGCTACCCGCAGGACCAAACAGTGACACTTCCCCTGGATGCCAAAGTCCTGGGGAGCA
  AAGATGCCACCCAGGCAGCAGCAGCAGATGAGAGCCACCCCCAAAGTTCGGACCTTATCAGAAGCCTTGA
  GGAAATCAGATATTCTTGTTGCCTTTTGCTAATGATCGGGCACTTCTGCAGCTGCAGAAGTTGAACCGCA
  ATGCTGCAGACCTGTGACCGCAGAATGCGACCAGAGAATGCCTGCAAGCTTCTCAGGCAAACTGCAGCAG
  GAGCATCAAGCCGCCTGGAAGTCACTCAGCAGAATGGCCTACCTGAACTAGGAATTGACCAATTGGCCGG
  TTCATTACCAAACCTCCAGGAAGGCTCTAGTGCCACCATGGACCGAGGCCACTCTCAAGGAACCGGCATT
  CTTTGGTCTCAACCACTGAACGGTACCCTCAAAGGGCAAGCTTTGGCTGTAAGGCCCAAGGCTTGAGAAT
  TGCTGCGTCATTGGTAATCTTTCCGAATGGCCGAAGGCGGCAAGACAGAACTCGAGGGTCATGGTGCACG
  TTAGAGCACCAGGCACATCG
  
  Kinase domain sequence

  >SC323550 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AAWGGMAGTCTATGTTTGTCAGATCAAAACCCTGGGGATCGGTGCCTTTGGAGAAGTGTGCCTTGCTTGT
  AAGGTGGACACTCACGCCCTGTACGCCATGATGACCCTAAGGAAAAAGGATGTCCTGAACCGGAATCAGG
  TGGCCCACGTCAAGGCCGAGAGGGACATCCTGGCCGAGGCAGACAATGAGTGGGTGGTCAAACTCTACTA
  CTCCTTCCAAGACAAAGACAGCCTGTACTTTGTGATGGACTACAT
  
• Restriction Sites: Please inquire
• ACCN: NM_014572
• ORF Size: 3267 bp
• Insert Size: 4200
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_014572.1, NP_055387.1
• RefSeq Size: 4098
• RefSeq ORF: 3267
• Locus ID: 26524
• Domains: UBA, pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008]