PKC zeta (PRKCZ) (NM_002744) Human Untagged Clone

CAT#: SC323556

PRKCZ (untagged)-Kinase deficient mutant (K281M) of Human protein kinase C, zeta (PRKCZ), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKCZ
• Synonyms: PKC-ZETA; PKC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323556 sequence for NM_002744 edited (data generated by NextGen Sequencing)
  ATGCCCAGCAGGACCGGCCCCAAGATGGAAGGGAGCGGCGGCCGCGTCCGCCTCAAGGCG
  CATTACGGGGGGGACATCTTCATCACCAGCGTGGACGCCGCCACGACCTTCGAGGAGCTC
  TGTGAGGAAGTGAGAGACATGTGTCGTCTGCACCAGCAGCACCCGCTCACCCTCAAGTGG
  GTGGACAGCGAAGGTGACCCTTGCACGGTGTCCTCCCAGATGGAGCTGGAAGAGGCTTTC
  CGCCTGGCCCGTCAGTGCAGGGACGAAGGCCTCATCATTCATGTTTTCCCGAGCACCCCT
  GAGCAGCCTGGCCTGCCATGTCCGGGAGAAGACAAATCTATCTACCGCCGGGGAGCCAGA
  AGATGGAGGAAGCTGTACCGTGCCAACGGCCACCTCTTCCAAGCCAAGCGCTTTAACAGG
  AGAGCGTACTGCGGTCAGTGCAGCGAGAGGATATGGGGCCTCGCGAGGCAAGGCTACAGG
  TGCATCAACTGCAAACTGCTGGTCCATAAGCGCTGCCACGGCCTCGTCCCGCTGACCTGC
  AGGAAGCATATGGATTCTGTCATGCCTTCCCAAGAGCCTCCAGTAGACGACAAGAACGAG
  GACGCCGACCTTCCTTCCGAGGAGACAGATGGAATTGCTTACATTTCCTCATCCCGGAAG
  CATGACAGCATTAAAGACGACTCGGAGGACCTTAAGCCAGTTATCGATGGGATGGATGGA
  ATCAAAATCTCTCAGGGGCTTGGGCTGCAGGACTTTGACCTAATCAGAGTCATCGGGCGC
  GGGAGCTACGCCAAGGTTCTCCTGGTGCGGTTGAAGAAGAATGACCAAATTTACGCCATG
  ATGGTGGTGAAGAAAGAGCTGGTGCATGATGACGAGGATATTGACTGGGTACAGACAGAG
  AAGCACGTGTTTGAGCAGGCATCCAGCAACCCCTTCCTGGTCGGATTACACTCCTGCTTC
  CAGACGACAAGTCGGTTGTTCCTGGTCATTGAGTACGTCAACGGCGGGGACCTGATGTTC
  CACATGCAGAGGCAGAGGAAGCTCCCTGAGGAGCACGCCAGGTTCTACGCGGCCGAGATC
  TGCATCGCCCTCAACTTCCTGCACGAGAGGGGGATCATCTACAGGGACCTGAAGCTGGAC
  AACGTCCTCCTGGATGCGGACGGGCACATCAAGCTCACAGACTACGGCATGTGCAAGGAA
  GGCCTGGGCCCTGGTGACACAACGAGCACTTTCTGCGGAACCCCGAATTACATCGCCCCC
  GAAATCCTGCGGGGAGAGGAGTACGGGTTCAGCGTGGACTGGTGGGCGCTGGGAGTCCTC
  ATGTTTGAGATGATGGCCGGGCGCTCCCCGTTCGACATCATCACCGACAACCCGGACATG
  AACACAGAGGACTACCTTTTCCAAGTGATCCTGGAGAAGCCCATCCGGATCCCCCGGTTC
  CTGTCCGTCAAAGCCTCCCATGTTTTAAAAGGATTTTTAAATAAGGACCCCAAAGAGAGG
  CTCGGCTGCCGGCCACAGACTGGATTTTCTGACATCAAGTCCCACGCGTTCTTCCGCAGC
  ATAGACTGGGACTTGCTGGAGAAGAAGCAGGCGCTCCCTCCATTCCAGCCACAGATCACA
  GACGACTACGGTCTGGACAACTTTGACACACAGTTCACCAGCGAGCCCGTGCAGCTGACC
  CCAGACGATGAGGATGCCATAAAGAGGATCGACCAGTCAGAGTTCGAAGGCTTTGAGTAT
  ATCAACCCATTATTGCTGTCCACCGAGGAGTCGGTGTGA
  
  Clone variation with respect to NM_002744.4
  264 t=>c;842 a=>t;843 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002744 unedited
  ACGCCGTTGAGCAATGGTCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCATGGCCGGAGC
  TCCCGGGGCGCAGCGCTGACGGCGGCGGGGGGAGCGCGCCATGCCCAGCAGGACCGGCCCCAAGATGGAA
  GGGAGCGGCGGCCGCGTCCGCCTCAAGGCGCATTACGGGGGGGACATCTTCATCACCAGCGTGGACGCCG
  CCACGACCTTCGAGGAGCTCTGTGAGGAAGTGAGAGACATGTGTCGTCTGCACCCAGCAGCACCCCGCTC
  ACCCTCAAGTGGGGTGGGACAGCGGAAGGGTGACCCTTGCACGGTGTCCCTCCCCAGATGGGAGGCTTGG
  AGAGGGCTTTTCCGCCCTGCCCCGTCCAGTGGCAGGGGACGGAGGGCCTTCATCCATTCCAGGTTTTTCC
  GGAGCCACCCCCTGAACCAGCCCGGCCCTGCATGTGTCCGGGAAAAAAACAATTTCATTTCTACCGCCGG
  GGGACCCGAAGAAATGGAGGAACCTTTACCGTGCAACCGGCCCCCCTTTCCAAGCCAGCCCCTTTAACAG
  AGAGAGCACCCCGGGGCACTGCAAACCGAGGGGATAGGGGGCTCCGCGAAACACGGGGCTACAAGTTAAC
  TCACCCCACACATCTTTTGGGTTACATAACGCCGTGCACAGCGCTCCTCTCCCTGATATCCTGGGAGAAC
  CATAGAGTATTGTGCTGCCGCCTCCAGAGCCCCTCTGTACACCAAAAACGAAGACGCACCTCTTTCTCCA
  GAGAAACAGAGGAGCTTCATATATCTCCTCCGCGAGACGCGAGCCCTCTTAACGCACGCTCGAGCACTCA
  CCTTCTTCGTGGATGGAGCATATCTCTGCGCTGCTGCGCAGTATCTATCAAGATTTAGCGAGCTGTATCA
  GAATGTCTGTCAGCGGTGTAGCAGAAGGCAC
  
  Kinase domain sequence

  >SC323556 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CGGGCTGAGCTGAGGACTTTGACCTAATCAGAGTCATCGGGCGCGGGAGCTACGCCAAGGTTCTCCTGGT
  GCGGTTGAAGAAGAATGACCAAATTTACGCCATGATGGTGGTGAATGACCAAATTTACGCCATGATGGTG
  GTGAAGAAGAATGACCAAATTTACGCCATGATGGTGGTGAAGAAAGAGCTGGTGCATGATGACGAGGATA
  TTGACTGGGTACAGACAGAGAAGCACGTGTTTGAGCAGGCATCCA
  
• Restriction Sites: Please inquire
• ACCN: NM_002744
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002744.3, NP_002735.3
• RefSeq Size: 2326 bp
• RefSeq ORF: 1779 bp
• Locus ID: 5590
• Cytogenetics: 1p36.33
• Domains: PB1, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis, Insulin signaling pathway, Tight junction, Type II diabetes mellitus
• Gene Summary: 'Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).