MAP4K3 (NM_003618) Human Untagged Clone

CAT#: SC323563

MAP4K3 (untagged)-Kinase deficient mutant (K45M) of Human mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP4K3
• Synonyms: GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003618, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGACTTCGAGCTGATTCAGCGCATCGGCA
  GCGGCACCTACGGCGACGTCTACAAGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAAT
  AAAATTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATTATGATGAAAGACTGTAAACAC
  CCAAATATTGTTGCTTATTTTGGAAGCTATCTCAGGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTG
  GAGGTGGTTCTTTACAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATATGTTAG
  CAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAAAAATGCACAGAGATATAAAGGGAGCT
  AACATTCTATTAACGGATAATGGTCATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTA
  CAATTGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAGTTGCAGCTGTTGAGAG
  GAAGGGGGGTTACAATCAACTCTGTGATCTCTGGGCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTT
  CAGCCTCCTATGTTTGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTCAGCCTC
  CTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCACTTTGTGAAAATGGCACTTACCAAAAA
  TCCGAAAAAAAGACCTACTGCTGAAAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCT
  TTGGCAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCATGATTTCGATGATGATG
  ATCCTGAGCCTCTTGTTGCTGTACCACATAGAATTCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAAC
  ACGCTCAGAGATAACCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCACATCAT
  GAACTTCCCGACAGTGATGGTTTTTTGGACAGTTCAGAAGAAATATACTACACTGCAAGATCTAATCTGG
  ATCTGCAACTGGAATATGGACAAGGACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTCTTCTCAA
  GTCTGTTGAAGAAGAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGATGAT
  GAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCCACCAAAGCCTAAGTCTATCTTCA
  TACCACAGGAAATGCATTCTACTGAGGATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTCAGGGAG
  CCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACACAAACCTGTTGCC
  TTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTGAACGAGATGGCTCATTATGTCAACAACAGAATG
  AACATAGAGGCACAAACCTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCTTCC
  TCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTAATGGGTGTCCCTTGAAAATTCAC
  TGTGCATCATCATGGATAAACCCAGATACAAGAGATCAGTACTTGATATTTGGTGCCGAAGAAGGGATTT
  ATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAAGGTGTACATGGTT
  GTATGTAATGAACAATTGCTTGCTATCAATATCTGGTAAAGCTTCTCAGCTTTATTCCCATAATTTACCA
  GGGCTTTTTGATTATGCAAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAAACTCCCTGACA
  GAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACCAAATGGTGCCAGAAGTGTTGTGT
  TGTAAGAAATCCTTACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAA
  TGGGTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCATGTCCACTTAGAA
  TGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCCTTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGA
  CTTCAACCAAGTGGTTCGATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGAATCAGAT
  ACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATACCATCCTTGTATGCTTGGACTGTT
  GTATAAAAATAGTAAATCTCCAAGGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGA
  TTTCCAGATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACATGGAATGCAAGGT
  AGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTTCAGATAGCACAAGAATTTTCAGGCTGCTTGGAT
  CTGACAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGTACATCCT
  GGCGGGTCATGAAAACAGTTACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003618 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCGGCGGCGGC
  GGCGGCGGCCGCAGCGTGGAGCCGGGTGATTGTGACCGCCGGGGGAGCCGGGAGCCGGCCGCGGCGCTCT
  CTCTCCGTGTGGCCCCCTGAGCGGCCCCCCTCCCCTGCCCGGGAGGGAGGCGGGGGGCACCTGGGGCCCG
  CCATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGAGACTTCGAGCTGATTCAGCGCATCGGGC
  AGCCGGCCCCTACGGCGACGTCTTACAAGGCCACGGAAGGTTACAACGGGGGAATTTACCAGCAATTATG
  GGTAATAAAAAATTGGACCCAGGAAAAAAACTTTGCATTGGTGCACCAGGAAATTTATCATGAAGGAAGA
  AATTGTAACCCCCCCAATTTTTGTTGTTTAATTTTGGAAACCATTCCCCAGGGCAAAAAAAAGCTTTGGA
  ATTTGGCAGGGGGTTTTTTGGGGGGGGGGGTTTTTTAACAGGGAATTTTTCCCCGGGATGGGCCCCCAAA
  TTTCCGATCGTTTTGAAAAGGAAGGGGGGTTTTACATCCCCTCTGTGGTCTTCCTGGGGACCGGGGGGAA
  AACTGTGCCCAAAAAATTTGGAAAACCTTCCCCCCCCCCGTTTGTGGACCTCTACCCCACAAGACGACTT
  ATTTTTTCATTGGAACTACCCTTTTTCCCCCCCTATCAAATAGCGAATAACAATAAAGAGGACACATTTT
  TTTATCCATATCGGTAGAAAGCGGGTATCAAAATATATCCCAAAAAGAACACAGCGTT
  
  Kinase domain sequence

  >SC323563 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CMTTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCGGCGGCGGCGGCGG
  CGGCCGCAGCGTGGAGCCGGGTGATTGTGACCGCCGGGGGAGCCGGGAGCCGGCCGCGGCGCTCTCTCTC
  CGTGTGGCCCCCTGAGCGGCCCCCCTCCCCTGCCCGGGAGGGAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_003618
• ORF Size: 2685 bp
• Insert Size: 4600
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003618.2, NP_003609.2
• RefSeq Size: 4141
• RefSeq ORF: 2685
• Locus ID: 8491
• Domains: pkinase, CNH, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.