MAP4K3 (NM_003618) Human Untagged Clone

CAT#: SC323563

MAP4K3 (untagged)-Kinase deficient mutant (K45M) of Human mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3)


  "NM_003618" in other vectors (7)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MAP4K3
Synonyms GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003618, the custom clone sequence may differ by one or more nucleotides


ATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGACTTCGAGCTGATTCAGCGCATCGGCA
GCGGCACCTACGGCGACGTCTACAAGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAAT
AAAATTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATTATGATGAAAGACTGTAAACAC
CCAAATATTGTTGCTTATTTTGGAAGCTATCTCAGGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTG
GAGGTGGTTCTTTACAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATATGTTAG
CAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAAAAATGCACAGAGATATAAAGGGAGCT
AACATTCTATTAACGGATAATGGTCATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTA
CAATTGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAGTTGCAGCTGTTGAGAG
GAAGGGGGGTTACAATCAACTCTGTGATCTCTGGGCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTT
CAGCCTCCTATGTTTGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTCAGCCTC
CTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCACTTTGTGAAAATGGCACTTACCAAAAA
TCCGAAAAAAAGACCTACTGCTGAAAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCT
TTGGCAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCATGATTTCGATGATGATG
ATCCTGAGCCTCTTGTTGCTGTACCACATAGAATTCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAAC
ACGCTCAGAGATAACCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCACATCAT
GAACTTCCCGACAGTGATGGTTTTTTGGACAGTTCAGAAGAAATATACTACACTGCAAGATCTAATCTGG
ATCTGCAACTGGAATATGGACAAGGACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTCTTCTCAA
GTCTGTTGAAGAAGAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGATGAT
GAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCCACCAAAGCCTAAGTCTATCTTCA
TACCACAGGAAATGCATTCTACTGAGGATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTCAGGGAG
CCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACACAAACCTGTTGCC
TTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTGAACGAGATGGCTCATTATGTCAACAACAGAATG
AACATAGAGGCACAAACCTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCTTCC
TCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTAATGGGTGTCCCTTGAAAATTCAC
TGTGCATCATCATGGATAAACCCAGATACAAGAGATCAGTACTTGATATTTGGTGCCGAAGAAGGGATTT
ATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAAGGTGTACATGGTT
GTATGTAATGAACAATTGCTTGCTATCAATATCTGGTAAAGCTTCTCAGCTTTATTCCCATAATTTACCA
GGGCTTTTTGATTATGCAAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAAACTCCCTGACA
GAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACCAAATGGTGCCAGAAGTGTTGTGT
TGTAAGAAATCCTTACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAA
TGGGTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCATGTCCACTTAGAA
TGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCCTTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGA
CTTCAACCAAGTGGTTCGATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGAATCAGAT
ACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATACCATCCTTGTATGCTTGGACTGTT
GTATAAAAATAGTAAATCTCCAAGGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGA
TTTCCAGATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACATGGAATGCAAGGT
AGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTTCAGATAGCACAAGAATTTTCAGGCTGCTTGGAT
CTGACAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGTACATCCT
GGCGGGTCATGAAAACAGTTACTGA


>OriGene 5' read for mutant NM_003618 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCGGCGGCGGC
GGCGGCGGCCGCAGCGTGGAGCCGGGTGATTGTGACCGCCGGGGGAGCCGGGAGCCGGCCGCGGCGCTCT
CTCTCCGTGTGGCCCCCTGAGCGGCCCCCCTCCCCTGCCCGGGAGGGAGGCGGGGGGCACCTGGGGCCCG
CCATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGAGACTTCGAGCTGATTCAGCGCATCGGGC
AGCCGGCCCCTACGGCGACGTCTTACAAGGCCACGGAAGGTTACAACGGGGGAATTTACCAGCAATTATG
GGTAATAAAAAATTGGACCCAGGAAAAAAACTTTGCATTGGTGCACCAGGAAATTTATCATGAAGGAAGA
AATTGTAACCCCCCCAATTTTTGTTGTTTAATTTTGGAAACCATTCCCCAGGGCAAAAAAAAGCTTTGGA
ATTTGGCAGGGGGTTTTTTGGGGGGGGGGGTTTTTTAACAGGGAATTTTTCCCCGGGATGGGCCCCCAAA
TTTCCGATCGTTTTGAAAAGGAAGGGGGGTTTTACATCCCCTCTGTGGTCTTCCTGGGGACCGGGGGGAA
AACTGTGCCCAAAAAATTTGGAAAACCTTCCCCCCCCCCGTTTGTGGACCTCTACCCCACAAGACGACTT
ATTTTTTCATTGGAACTACCCTTTTTCCCCCCCTATCAAATAGCGAATAACAATAAAGAGGACACATTTT
TTTATCCATATCGGTAGAAAGCGGGTATCAAAATATATCCCAAAAAGAACACAGCGTT
>SC323563 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CMTTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCGGCGGCGGCGGCGG
CGGCCGCAGCGTGGAGCCGGGTGATTGTGACCGCCGGGGGAGCCGGGAGCCGGCCGCGGCGCTCTCTCTC
CGTGTGGCCCCCTGAGCGGCCCCCCTCCCCTGCCCGGGAGGGAGG
Restriction Sites Please inquire     
ACCN NM_003618
ORF Size 2685 bp
Insert Size 4600
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Reference Data
RefSeq NM_003618.2, NP_003609.2
RefSeq Size 4141
RefSeq ORF 2685
Locus ID 8491
Domains pkinase, CNH, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways MAPK signaling pathway
Gene Summary This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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