MAP4K5 (NM_006575) Human Untagged Clone

CAT#: SC323591

MAP4K5 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP4K5
• Synonyms: GCKR; KHS; KHS1; MAPKKKK5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006575, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGCCCCGCTGCGGCCTGCCGCGGACATCCTGAGGCGGAACCCGCAGCAGGACTACGAACTCGTCC
  AGAGGGTCGGCAGCGGCACCTACGGGGACGTCTATAAGGCCAGAAATGTACACACAGGAGAGCTGGCTGC
  AGTAAAAATCATTAAATTGGAGCCTGGAGATGATTTTTCTTTGATTCAACAAGAAATATTTATGGTTAAA
  GAATGTAAACATTGTAACATCGTTGCCTACTTTGGGAGTTATCTTAGTCGGGAAAAACTATGGATTTGTA
  TGGAATACTGTGGTGGCGGATCACTTCAAGATATTTACCATGTTACTGGACCATTATCAGAATTGCAAAT
  AGCCTATGTATGCAGAGAAACCTTACAGGGTCTTGCCTATTTGCATACTAAAGGCAAAATGCATAGAGAT
  ATCAAAGGTGCTAATATTTTATTGACAGACCATGGCGATGTAAAATTAGCTGACTTTGGTGTGGCTGCAA
  AAATAACAGCTACCATTGCAAAACGAAAATCTTTCATTGGCACCCCTTACTGGATGGCCCCAGAAGTTGC
  AGCAGTAGAGAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCAGTAGGAATAACAGCAATTGAA
  CTTGGAGAACTTCAGCCACCTATGTTTGATCTCCACCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTA
  ATTTTCAGCCTCCAAAACTAAAGGACAAAACAAAATGGTCATCAACATTCCATAATTTTGTCAAAATAGC
  ACTAACCAAAAACCCAAAAAAAAGACCAACTGCTGAAAGACTTCTGACTCACACTTTTGTTGCACAGCCA
  GGTCTCTCTAGAGCCCTAGCAGTTGAACTGTTAGACAAAGTGAACAATCCAGATAACCACGCACATTACA
  CTGAAGCAGATGACGATGACTTTGAGCCCCATGCAATCATTCGTCATACCATTAGATCTACAAACAGGAA
  TGCCAGAGCTGAACGGACAGCTTCAGAAATAAATTTTGACAAATTACAATTTGAACCTCCTCTGAGAAAA
  GAAACAGAAGCACGAGATGAAATGGGATTGTCATCAGACCCAAATTTCATGTTACAGTGGAATCCTTTTG
  TTGATGGTGCAAATACTGGCAAATCAACCTCAAAACGTGCAATACCACCTCCCCTACCTCCTAAGCCAAG
  GATAAGCAGTTACCCTGAAGACAACTTTCCGGATGAAGAAAAAGCATCAACCATAAAACATTGTCCTGAT
  TCAGAAAGCAGAGCTCCCCAAATTCTCAGAAGACAGAGTAGCCCAAGTTGTGGGCCTGTGGCAGAGACTT
  CTTCTATTGGAAATGGTGATGGTATTTCAAAACTGATGAGTGAAAATACAGAAGGATCAGCACAAGCACC
  ACAGTTACCACGAAAAAAGGACAAACGAGACTTCCCTAAACCAGCCATCAATGGCCTTCCACCCACCCCA
  AAAGTTCTGATGGGAGCATGCTTTTCAAAAGTTTTTGATGGCTGTCCTTTGAAAATTAATTGTGCAACAT
  CCTGGATACATCCTGATACAAAAGATCAGTACATTATTTTTGGAACTGAAGATGGTATTTACACACTGAA
  TCTCAATGAGCTACATGAGGCAACGATGGAACAGTTATTTCCACGGAAGTGTACTTGGCTGTATGTTATC
  AATAATACTTTAATGTCATTATCAGAAGGAAAAACCTTTCAGCTCTACTCTCACAATCTTATAGCTTTGT
  TTGAACATGCCAAAAAACCAGGATTAGCTGCCCATATTCAAACTCACAGGTTTCCAGACCGAATACTACC
  AAGAAAATTCGCTTTAACAACAAAGATTCCTGATACAAAAGGCTGCCACAAATGTTGCATAGTCAGAAAC
  CCTTACACGGGACATAAATACCTCTGTGGAGCTTTACAGTCTGGAATTGTTTTACTTCAGTGGTATGAGC
  CAATGCAGAAATTCATGTTGATAAAGCACTTTGATTTTCCTTTGCCAAGTCCTTTGAATGTTTTTGAAAT
  GCTGGTGATACCTGAACAGGAATACCCTATGGTCTGTGTAGCTATTAGCAAAGGCACTGAATCGAATCAG
  GTAGTTCAGTTTGAGACAATCAATTTGAACTCTGCATCTTCATGGTTTACAGAAATTGGTGCAGGCAGCC
  AGCAGTTAGATTCCATTCATGTAACACAGTTGGAGAGAGATACCGTTTTAGTGTGTTTAGACAAATTTGT
  GAAAATTGTAAATCTACAAGGAAAATTAAAATCAAGTAAGAAACTGGCCTCTGAGTTAAGTTTTGATTTT
  CGCATTGAATCTGTAGTATGCCTTCAAGACAGTGTGTTGGCTTTCTGGAAACATGGGATGCAGGGTAAAA
  GCTTCAAGTCAGATGAGGTTACCCAGGAGATTTCAGATGAAACAAGAGTTTTCCGCTTATTAGGATCAGA
  CAGGGTTGTCGTTTTGGAAAGTAGGCCAACAGAAAATCCTACTGCACACAGCAATCTCTACATCTTGGCT
  GGACATGAAAATAGTTACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_006575 unedited
  CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGTGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGGCGGCGACTGT
  ACGCGCCTCCGCCGCCCCCGAGAGGACGCGCCGTGCAGCGGCTGAGTGGCGGCGGCGGCGACGGCAAACC
  CGGAGCTGCCGGCCGGCGCGCGGGAGGAGGACGCGGGTGCGGTCTAGGAAACGGAGCTGCGGGCGGAGGC
  TCCATGTTGGGAAGCGGCGCCGTTCGTGCTTGTTAGCGGGAATCCGGGAGCCGCGGGGTGAGCTGGCCGG
  GGGGCCCGGGCCCTAAGTGAAGATGGAAGGCCCCCGCTGGCGGCCCTGCCGGCGGACATCCTGAGGCGGA
  CCCCGCAGCAGGACTTACGACCTCGTCCAGGAGGGTCGGCACCGGCACCTAACGGGGACGTCCTATAAGG
  CCGAAATGTACACACAGGAGAGCCTGGCTTGAATAATGAATCATTAAATTGAACCTGGAAGGTTATTTTC
  TTGAATTACACAGAATTTTTATGGTAAGAATGTACCATTGAACATCGGTGCATCTTGGGGAGTACTTATC
  GGAAACCTGGATGTAGGATACGGGGGGGTCTCAATTTCCAGTTCTGACATTCGATGCAATTCCTAGTTCG
  AAACTTAAGGTCTGCAATTGCTCACGCACCGTGAATCAGGTCTTTTTTTGGCACTCGGCGATAATAACAA
  TAGGTCAAATCCACTTGAAAAAATT
  
  Kinase domain sequence

  >SC323591 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ARTCAGAGGGTCGGAGCGGCACCTACGGGGACGTCTATAAGGCCAGAAATGTACACACAGGAGAGCTGGC
  TGCAGTAATGATCATTAAATTGGAGCCTGGAGATGATTTTTCTTTGATTCAACAAGAAATATTTATGGTT
  AAAGAATGTAAACATTGTAACATCGTTGCCTACTTTGGGAGTTATCTTAGTCGGGAAAAACTATGGATTT
  GTATGGAATACTGTGGTGGCGGATCACTTCAAGATATTTACCATG
  
• Restriction Sites: Please inquire
• ACCN: NM_006575
• ORF Size: 2541 bp
• Insert Size: 4310
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_006575.3, NP_006566.2
• RefSeq Size: 4373
• RefSeq ORF: 2541
• Locus ID: 11183
• Domains: pkinase, CNH
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform.