NLK (NM_016231) Human Untagged Clone

CAT#: SC323592

NLK (untagged)-Kinase deficient mutant (K155M) of Human nemo-like kinase (NLK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NLK
• Synonyms: DKFZp761G1211; FLJ21033
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016231, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTCTTTGTGGCGCAAGAGCCAACGCAAAAATGATGGCGGCTTACAATGGCGGTACATCTGCAGCAG
  CAGCAGGTCACCACCACCACCATCACCACCACCTTCCACACCTCCCTCCTCCTCACCTGCACCACCACCA
  CCACCCTCAACACCATCTTCATCCGGGGTCGGCTGCCGCTGTACACCCTGTACAGCAGCACACCTCTTCG
  GCAGCTGCGGCAGCCGCAGCAGCGGCTGCAGCTGCAGCCATGTTAAACCCTGGGCAACAACAGCCATATT
  TCCCATCACCGGCACCGGGGCAGGCTCCTGGACCAGCTGCAGCAGCCCCAGCTCAGGTACAGGCTGCCGC
  AGCTGCTACAGTTAAGGCGCACCATCATCAGCACTCGCATCATCCACAGCAGCAGCTGGATATTGAGCCG
  GATAGACCTATTGGATATGGAGCCTTTGGTGTTGTCTGGTCAGTAACAGATCCAAGAGATGGAAAGAGAG
  TAGCGCTCAAAAAGATGCCCAACGTCTTCCAGAATCTGGTCTCTTGCAAAAGGGTCTTCCGGGAATTGAA
  GATGTTGTGTTTTTTTAAGCATGATAATGTACTCTCTGCCCTTGACATACTCCAACCTCCACACATTGAC
  TATTTTGAAGAAATATATGTTGTCACAGAATTGATGCAGAGTGACCTACATAAAATTATCGTCTCTCCTC
  AACCACTCAGCTCAGATCATGTCAAAGTTTTTCTTTATCAGATTTTGCGAGGTTTGAAATATCTCCATTC
  AGCTGGCATTTTACATCGAGACATTAAGCCAGGGAATCTCCTTGTGAACAGCAACTGTGTTCTAAAGATT
  TGTGATTTTGGATTGGCCAGAGTGGAAGAATTAGATGAATCCCGTCATATGACTCAGGAAGTTGTTACTC
  AGTATTATCGGGCTCCAGAAATCCTGATGGGCAGCCGTCATTACAGCAATGCTATTGACATCTGGTCTGT
  GGGATGTATCTTTGCAGAACTACTAGGACGAAGAATATTGTTTCAGGCACAGAGTCCCATTCAGCAGTTG
  GATTTGATCACGGATCTGTTGGGCACACCATCACTGGAAGCAATGAGGACAGCTTGTGAAGGCGCTAAGG
  CACATATACTCAGGGGTCCTCATAAACAGCCATCTCTTCCTGTACTCTATACCCTGTCTAGCCAGGCTAC
  ACATGAAGCTGTTCATCTCCTTTGCAGGATGTTGGTCTTTGATCCATCCAAAAGAATATCCGCTAAGGAT
  GCCTTAGCCCACCCCTACCTAGATGAAGGGCGACTACGATATCACACATGTATGTGTAAATGTTGCTTTT
  CCACCTCCACTGGAAGAGTTTATACCAGTGACTTTGAGCCTGTCACCAATCCCAAATTTGATGACACTTT
  CGAGAAGAACCTCAGTTCTGTCCGACAGGTTAAAGAAATTATTCATCAGTTCATTTTGGAACAGCAGAAA
  GGAAACAGAGTGCCTCTCTGCATCAACCCTCAGTCTGCTGCTTTTAAGAGCTTTATTAGTTCCACTGTTG
  CTCAGCCATCTGAGATGCCCCCATCTCCTCTGGTGTGGGAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_016231 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGTCCTTTTTTC
  CTTTTTCTTTTCTTTTTTCCCTTTTTTTTCCTTTTGGCAACCCACACCCTTCCACACACGCTCACCCCAA
  AATTAAACACCAAGATCCTCTAACTTGTTTGGATTGACTGATGAAGACATAAAGCTCTATGTTTTTTGAG
  GTGGAGTGAGTGGTTTTTCTTCATTTTTAAATGGCCAAATGACAGCTTGACCCAGTTTGCTTTCCAATCA
  AAGGGCATTTTATTTTGAATGTCTCTTTGTGGCGCAAGAGCCACCGCAAAAATGATGGGCGGCTTACAAT
  GGCGGTCATCTGCAGCAGCAGCAGGTACCACCACCACCATACCACCACCTTCCAACTCCTTCTCCTAACT
  GAACACCACCACACCTAAAACATTTTATCGGGGTGGCTGCGCTTACCCCGGAAAGAGCAACCTTGGAGTG
  GGAACCAAAACGCTGATTGCACATTAACCTGGAACACACCAATTCCACCGCCGGGCAGGCCTGACATCGC
  AACCCATTAGACGGCGCGACTCATAAGGCCACATACGCTGCACTCAGACGGTTGCGCGACCTGATAGCTT
  GGTCGGTCAATCAAGAAAGCCGAGACCTCTCATGTTGAAGTCGAGAAAGTGTTACAGCCCCCCCCCCCCC
  CCTTAAAAA
  
  Kinase domain sequence

  >SC323592 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTCMAGCAGCAGCTGGWATTGAGCCGGATAGACCTATTGGATATGGAGCCTTTGGTGTTGTCTGGTCAGT
  AACAGATCCAAGAGATGGAAAGAGAGTAGCGCTCATGAAGATGCCCAACGTCTTCCAGAATCTGGTCTCT
  TGCAAAAGGGTCTTCCGGGAATTGAAGATGTTGTGTTTTTTTAAGCATGATAATGTACTCTCTGCCCTTG
  ACATACTCCAACCTCCACACATTGACTATTTTGAAGAAATATATG
  
• Restriction Sites: Please inquire
• ACCN: NM_016231
• ORF Size: 3555 bp
• Insert Size: 3800
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_016231.2, NP_057315.2
• RefSeq Size: 3555
• RefSeq ORF: 3555
• Locus ID: 51701
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Adherens junction, MAPK signaling pathway, Wnt signaling pathway