Protein Kinase D2 (PRKD2) (NM_016457) Human Untagged Clone

CAT#: SC323600

PRKD2 (untagged)-Kinase deficient mutant (K580M) of Human protein kinase D2 (PRKD2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKD2
• Synonyms: HSPC187; nPKC-D2; PKD2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323600 sequence for NM_016457 edited (data generated by NextGen Sequencing)
  ATGGCCACCGCCCCCTCTTATCCCGCCGGGCTCCCTGGCTCTCCCGGGCCGGGGTCTCCT
  CCGCCCCCCGGCGGCCTAGAGCTGCAGTCGCCGCCACCGCTACTGCCCCAGATCCCGGCC
  CCGGGTTCCGGGGTCTCCTTTCACATCCAGATCGGGCTGACCCGCGAGTTCGTGCTGTTG
  CCCGCCGCCTCCGAGCTGGCTCATGTGAAGCAGCTGGCCTGTTCCATCGTGGACCAGAAG
  TTCCCTGAGTGTGGCTTCTACGGCCTTTACGACAAGATCCTGCTTTTCAAACATGACCCC
  ACGTCGGCCAACCTCCTGCAGCTGGTGCGCTCGTCCGGAGACATCCAGGAGGGCGACCTG
  GTGGAGGTGGTGCTGTCGGCCTCGGCCACCTTCGAGGACTTCCAGATCCGCCCGCACGCC
  CTCACGGTGCACTCCTATCGGGCGCCTGCCTTCTGTGATCACTGCGGGGAGATGCTCTTC
  GGCCTAGTGCGCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAACTACCACAAGCGCTGT
  GCCTTCAGCATCCCCAACAACTGTAGTGGGGCCCGCAAACGGCGCCTGTCATCCACGTCT
  CTGGCCAGTGGCCACTCGGTGCGCCTCGGCACCTCCGAGTCCCTGCCCTGCACGGCTGAA
  GAGCTGAGCCGTAGCACCACCGAACTCCTGCCTCGCCGTCCCCCGTCATCCTCTTCCTCC
  TCTTCTGCCTCATCGTATACGGGCCGCCCCATTGAGCTGGACAAGATGCTGCTCTCCAAG
  GTCAAGGTGCCGCACACCTTCCTCATCCACAGCTATACACGGCCCACCGTTTGCCAGGCT
  TGCAAGAAACTCCTCAAGGGCCTCTTCCGGCAGGGCCTGCAATGCAAAGACTGCAAGTTT
  AACTGTCACAAACGCTGCGCCACCCGCGTCCCTAATGACTGCCTGGGGGAGGCCCTTATC
  AATGGAGATGTGCCGATGGAGGAGGCCACCGATTTCAGCGAGGCTGACAAGAGCGCCCTC
  ATGGATGAGTCAGAGGACTCCGGTGTCATCCCTGGCTCCCACTCAGAGAATGCGCTCCAC
  GCCAGTGAGGAGGAGGAAGGCGAGGGAGGCAAGGCCCAGAGCTCCCTGGGGTACATCCCC
  CTAATGAGGGTGGTGCAATCGGTGCGACACACGACGCGGAAATCCAGCACCACGCTGCGG
  GAGGGTTGGGTGGTTCATTACAGCAACAAGGACACGCTGAGAAAGCGGCACTATTGGCGC
  CTGGACTGCAAGTGTATCACGCTCTTCCAGAACAACACGACCAACAGATACTATAAGGAA
  ATTCCGCTGTCAGAAATCCTCACGGTGGAGTCCGCCCAGAACTTCAGCCTTGTGCCGCCG
  GGCACCAACCCACACTGCTTTGAGATCGTCACTGCCAATGCCACCTACTTCGTGGGCGAG
  ATGCCTGGCGGGACTCCGGGTGGGCCAAGTGGGCAGGGGGCTGAGGCCGCCCGGGGCTGG
  GAGACAGCCATCCGCCAGGCCCTGATGCCCGTCATCCTTCAGGACGCACCCAGCGCCCCA
  GGCCACGCGCCCCACAGACAAGCTTCTCTGAGCATCTCTGTGTCCAACAGTCAGATCCAA
  GAGAATGTGGACATTGCCACTGTCTACCAGATCTTCCCTGACGAAGTGCTGGGCTCAGGG
  CAGTTTGGAGTGGTCTATGGAGGAAAACACCGGAAGACAGGCCGGGACGTGGCAGTTAAG
  GTCATTGACAAACTGCGCTTCCCTACCAAGCAGGAGAGCCAGCTCCGGAATGAAGTGGCC
  ATTCTGCAGAGCCTGCGGCATCCCGGGATCGTGAACCTGGAGTGCATGTTCGAGACGCCT
  GAGAAAGTGTTTGTGGTGATGGAGAAGCTGCATGGGGACATGTTGGAGATGATCCTGTCC
  AGTGAGAAGGGCCGGCTGCCTGAGCGCCTCACCAAGTTCCTCATCACCCAGATCCTGGTG
  GCTTTGAGACACCTTCACTTCAAGAACATTGTCCACTGTGACTTGAAACCAGAAAACGTG
  TTGCTGGCATCAGCAGACCCATTTCCTCAGGTGAAGCTGTGTGACTTTGGCTTTGCTCGC
  ATCATCGGCGAGAAGTCGTTCCGCCGCTCAGTGGTGGGCACGCCGGCCTACCTGGCACCC
  GAGGTGCTGCTCAACCAGGGCTACAACCGCTCGCTGGACATGTGGTCAGTGGGCGTGATC
  ATGTACGTCAGCCTCAGCGGCACCTTCCCTTTCAACGAGGATGAGGACATCAATGACCAG
  ATCCAGAACGCCGCCTTCATGTACCCGGCCAGCCCCTGGAGCCACATCTCAGCTGGAGCC
  ATTGACCTCATCAACAACCTGCTGCAGGTGAAGATGCGCAAACGCTACAGCGTGGACAAA
  TCTCTCAGCCACCCCTGGTTACAGGAGTACCAGACGTGGCTGGACCTCCGAGAGCTGGAG
  GGGAAGATGGGAGAGCGATACATCACGCATGAGAGTGACGACGCGCGCTGGGAGCAGTTT
  GCAGCAGAGCATCCGCTGCCTGGGTCTGGGCTGCCCACGGACAGGGATCTCGGTGGGGCC
  TGTCCACCACAGGACCACGACATGCAGGGGCTGGCGGAGCGCATCAGTGTTCTCTGA
  
  Clone variation with respect to NM_016457.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_016457 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCTCCCCCCATGGC
  CACCGCCCCCTCTTATCCCGCCGGGCTCCCTGGCTCTCCCGGGCCGGGGTCTCCTCCGCCCCCCGGCGGC
  CTAGAGCTGCAGTCGCCGCCACCGCTACTGCCCCAGATCCCGGCCCCGGGTTCCGGGGTCTCCTTTCACA
  TCCAGATCGGGCTGACCCGCGAGTTCGTGCTGTTGCCCGCCGCCTCCGAGCTGGGCTCATGTGAAGCAGC
  TGGCCTGTTCCCATCGTGGACCCAAAAGTTCCCTGAAGTGTGGCTTTCTACGGCCTTTACAACAAGATTC
  CTGGCTTTTTCAAACATGGACCCCACTTCGGCCAACCTCCGGCACTGGGGCCCCCTCGTCCGGAAACTTC
  CAGGAGGGCAAACTGGGTGGGAGGGGGGGGCTGTCGGCCCTCGGCCCCCTTCAGGGACTCCCAAATCCCC
  CCCCCCCCCCTCAGGGGGCTTTCTTATCGGGGCCCCGGCCTTTGGGGTTTCTGGGCGGGGGAAACGCTTT
  TTGGCCCATTTGCCCCGGGCCCTAATGTCCATTCTTTCCGCTCAAAAACTCCAAACGCCTTGGGCTTTCA
  TCCCCCACAACTGTGGTGGGGGGGCCCAAAAGGGGCCCGTGATCTCCTTTTTGTGGCCGGGGGCTCGTGG
  GGCCCCCTGGCCTCTAGAACCTCGCGCGCAGCGCGGGTAAAATATTTACCATAACCCACAAAACTCTGCG
  TGCCGGCCCCCCCAGACTCTTTCTCCTTTTGTGTGACATATAATAAGCCGCCCCACTATAGTGAGAAGAA
  AGATGGTGTCTCTGAGCAGGAGGTGGACGCACTCTTTTCTACTAGATATTATAGATCACAGTGTT
  
  Kinase domain sequence

  >SC323600 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGMTTGCACTGTCTACCAGATCTTCCCTGACGAAGTGCTGGGCTCAGGGCAGTTTGGAGTGGTCTATGGA
  GGAAAACACCGGAAGACAGGCCGGGACGTGGCAGTTATGGTCATTGACAAACTGCGCTTCCCTACCAAGC
  AGGAGAGCCAGCTCCGGAATGAAGTGGCCATTCTGCAGAGCCTGCGGCATCCCGGGATCGTGAACCTGGA
  GTGCATGTTCGAGACGCCTGAGAAAGTGTTTGTGGTGATGGAGAA
  
• Restriction Sites: Please inquire
• ACCN: NM_016457
• ORF Size: 921 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_016457.1, NP_057541.1
• RefSeq Size: 921
• RefSeq ORF: 921
• Locus ID: 25865
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same isoform (A).