CAMK2G (NM_172170) Human Untagged Clone

CAT#: SC323611

CAMK2G (untagged)-Kinase deficient mutant (K43M) of Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3


  "NM_172170" in other vectors (8)

Reconstitution Protocol

USD 880.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CAMK2G
Synonyms CAMK; CAMK-II; CAMKG
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_172170, the custom clone sequence may differ by one or more nucleotides


ATGGCCACCACCGCCACCTGCACCCGTTTCACCGACGACTACCAGCTCTTCGAGGAGCTTGGCAAGGGTG
CTTTCTCTGTGGTCCGCAGGTGTGTGAAGAAAACCTCCACGCAGGAGTACGCAGCAAAAATCATCAATAC
CAAGAAGTTGTCTGCCCGGGATCACCAGAAACTAGAACGTGAGGCTCGGATATGTCGACTTCTGAAACAT
CCAAACATCGTGCGCCTCCATGACAGTATTTCTGAAGAAGGGTTTCACTACCTCGTGTTTGACCTTGTTA
CCGGCGGGGAGCTGTTTGAAGACATTGTGGCCAGAGAGTACTACAGTGAAGCAGATGCCAGCCACTGTAT
ACATCAGATTCTGGAGAGTGTTAACCACATCCACCAGCATGACATCGTCCACAGGGACCTGAAGCCTGAG
AACCTGCTGCTGGCGAGTAAATGCAAGGGTGCCGCCGTCAAGCTGGCTGATTTTGGCCTAGCCATCGAAG
TACAGGGAGAGCAGCAGGCTTGGTTTGGTTTTGCTGGCACCCCAGGTTACTTGTCCCCTGAGGTCTTGAG
GAAAGATCCCTATGGAAAACCTGTGGATATCTGGGCCTGCGGGGTCATCCTGTATATCCTCCTGGTGGGC
TATCCTCCCTTCTGGGATGAGGATCAGCACAAGCTGTATCAGCAGATCAAGGCTGGAGCCTATGATTTCC
CATCACCAGAATGGGACACGGTAACTCCTGAAGCCAAGAACTTGATCAACCAGATGCTGACCATAAACCC
AGCAAAGCGCATCACGGCTGACCAGGCTCTCAAGCACCCGTGGGTCTGTCAACGATCCACGGTGGCATCC
ATGATGCATCGTCAGGAGACTGTGGAGTGTTTGCGCAAGTTCAATGCCCGGAGAAAACTGAAGGGTGCCA
TCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGG
CGGTGTCAAGCCACAGAGCAACAACAAAAACAGTCTCGTAAGCCCAGCCCAAGAGCCCGCGCCCTTGCAG
ACGGCCATGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCT
GCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCT
GATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTT
GAGCCTGAGGCCCTTGGTAACCTCGTGGAGGGGATGGATTTCCATAAGTTTTACTTTGAGAATCTCCTGT
CCAAGAACAGCAAGCCTATCCATACCACCATCCTAAACCCACACGTCCACGTGATTGGGGAGGACGCAGC
GTGCATCGCCTACATCCGCCTCACCCAGTACATCGACGGGCAGGGTCGGCCTCGCACCAGCCAGTCAGAA
GAGACCCGGGTCTGGCACCGTCGGGATGGCAAGTGGCTCAATGTCCACTATCACTGCTCAGGGGCCCCTG
CCGCACCGCTGCAGTGA


>OriGene 5' read for mutant NM_172170 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGGCGCGGGCT
GCACCGCCGCGTCCGCCCGCCCGCCAGCATGGCCACCACCGCCACCTGCACCCGTTTCACCGACGACTAC
CAGCTCTTCGAGGAGCTTGGCAAGGGTGCTTTCTCTGTGGTCCGCAGGTGTGTGAAGAAAACCTCCACGC
AGGAGTACGCAGCAATGATCATCAATACCAAGAAGTTGTCTGCCCGGGATCACCCAGAAAACTAGAACGG
GGAGGCTCGGATATGTCGACTTCTGAAAACATCCAAAACATCGGTGCCGCCTCCCATGAACAGTTATTTC
TTGAAGAAGGGTTTCACTACCTTGTTGTTTTGACCTTGTTACCGGCGGGGAAGCTTGTTTGGAAGACTTT
GTGGCCAGAGAGTACTACAGTGAACAGGATGCCAGCCCTGGTATACTTCGATTCTGGGAGAGTGTTAACC
CCTTCCCCCAGATTGACTTTGTCCCAGGGACCTGACCTTGAGAACGGGTTGCGGGCGATTAAAGGCAGGG
GTGCGCCCTAAGCTGGCTGATTTGGCCTACCCTCAATTACGGGGAGAGCACGCGCTTGTTGGTTGTGTGG
CACCAGTCTACTTGCCCGAGTGTGTGGAGAGCTCCTGGGAACCCTGTGAATCTGGCTCGCGGCATCTCTG
TAACCCCGTGGCGACATCTCTGGGAGAGATACACACACGTTCACTAACGGGGCCCTATCTCCCCCATGAG
CAGTATCTGGACGCAATGTTACACGGGCTATCAGACATCTGTCCACTTTAACCTGATGTAA
>SC323611 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AYRTTMMRMAAWGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACMRK
CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGGCGCGGGCTGCACC
GCCGCGTCCGCCCGCCCGCCAGCATGGCCACCACCGCCACCTGCACCCGTTTCACCGACGACTACCAGCT
CTTCRAGGAGCTTGGCAWGGGTGCTTTCTCTGTGGTCCGCAGGTG
Restriction Sites Please inquire     
ACCN NM_172170
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172170.2, NP_751910.1
RefSeq Size 3712 bp
RefSeq ORF 1557 bp
Locus ID 818
Cytogenetics 10q22.2
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway
Gene Summary 'The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined.[provided by RefSeq, Mar 2011]'
Transcript Variant: This variant (3) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (3), that is missing an internal segment compared to isoform 1. Variants 3 and 50 both encode the same isoform (3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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