TESK2 (NM_007170) Human Untagged Clone

CAT#: SC323613

TESK2 (untagged)-Kinase deficient mutant (K87M) of Human testis-specific kinase 2 (TESK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TESK2
• Synonyms: MGC29168; OTTMUSP00000010191; testis-specific kinase 2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007170, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCGGAGCAAACGGAATTCAATTGCAGGATTTCCTCCACGTGTGGAGCGTCTTGAAGAGTTTGAAG
  GAGGTGGTGGAGGAGAAGGAAATGTGAGCCAGGTGGGAAGAGTTTGGCCATCTTCGTATCGAGCTCTTAT
  AAGTGCCTTTTCCAGACTGACGCGTTTGGATGATTTCACCTGTGAAAAAATAGGGTCTGGCTTCTTTTCT
  GAAGTGTTCAAGGTACGACACCGAGCTTCTGGTCAGGTGATGGCTCTTAAGATGAACACATTGAGCAGTA
  ACCGGGCAAACATGCTGAAAGAAGTACAGCTCATGAATAGACTCTCCCATCCCAACATCCTTAGGTTCAT
  GGGTGTATGTGTTCATCAAGGACAATTGCATGCACTTACAGAGTATATCAACTCCGGGAACCTGGAACAG
  TTGCTAGACAGTAACCTGCATTTGCCTTGGACTGTGAGGGTAAAACTGGCCTATGACATAGCAGTGGGCC
  TCAGCTACCTTCACTTCAAAGGCATTTTTCATCGGGACCTCACATCTAAGAACTGCCTGATAAAGAGGGA
  TGAGAATGGTTACTCTGCAGTGGTAGCTGACTTTGGCCTGGCTGAGAAGATCCCCGATGTCAGCATGGGG
  AGTGAGAAGCTGGCCGTGGTGGGTTCCCCATTCTGGATGGCACCTGAGGTTCTCCGAGATGAGCCCTATA
  ATGAAAAGGCAGATGTGTTCTCTTATGGTATCATCCTCTGCGAGATCATCGCCCGCATCCAGGCCGATCC
  GGACTATCTTCCCCGCACAGAGAATTTCGGGCTGGACTATGATGCTTTCCAGCACATGGTGGGAGACTGT
  CCCCCAGATTTTCTGCAACTTACTTTCAACTGCTGTAACATGGATCCCAAACTGCGCCCATCTTTTGTGG
  AGATTGGGAAGACCCTGGAGGAAATTCTGAGCCGCCTACAGGAAGAAGAGCAGGAGAGGGATAGGAAGCT
  GCAGCCCACAGCCAGGGGACTCTTGGAGAAAGCACCTGGGGTGAAGCGACTAAGCTCACTGGATGACAAG
  ATCCCCCACAAGTCACCATGCCCAAGACGTACCATCTGGCTGTCTCGAAGCCAGTCAGATATCTTTTCCC
  GTAAGCCCCCACGTACAGTGAGTGTCTTGGACCCATACTACCGGCCACGAGATGGTGCTGCCCGCACCCC
  CAAAGTCAACCCTTTTAGTGCTCGCCAGGACCTCATGGGGGGCAAGATCAAGTTTTTTGACCTGCCCAGC
  AAGTCTGTCATCTCTCTGGTATTTGACCTGGATGCACCAGGGCCCGGAACTATGCCCCTGGCTGACTGGC
  AGGAGCCCCTGGCCCCACCTATTCGCCGGTGGCGTTCCTTGCCTGGTTCGCCTGAGTTCTTGCATCAAGA
  GGCTTGTCCATTTGTGGGCCGGGAAGAATCGCTATCTGATGGGCCCCCACCACGCCTAAGTAGTCTCAAG
  TACAGAGTTAAAGAGATCCCACCATTCCGGGCATCTGCCCTACCAGCTGCTCAAGCCCATGAGGCTATGG
  ACTGCTCCATTCTCCAGGAAGAAAATGGTTTTGGGTCCAGGCCCCAGGGGACCAGTCCATGCCCTGCGGG
  TGCTTCTGAGGAGATGGAGGTAGAAGAAAGGCCAGCAGGCTCAACTCCAGCCACCTTCTCCACCTCAGGC
  ATAGGCCTGCAAACCCAGGGAAAGCAGGATGGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_007170 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCCTGTTGGCTTCGC
  TGGTCGGATCGTCCTCCTGGCCCCGCCAAACAGGCGGGGGGAGCGGCCCCGACTGTGGGGCCATGGCCGT
  AGTCTCCTCGTTCTCCGCCGCCGCTAGCCTACCTGAGTCTCCGGCTTCTGCGCTAGGGGCTCCTACCGCC
  TCCGCAGGCTAGGAGCCGCTGCCCCACGAGCTGTGACGGTTACTAGCTCCTCTTGCCGCCGCCCCCCCCT
  TGCCCCCCCGCCCCGCGGGCTGCTCGTTTGCAAAAGCGACCTAGAAGAATAAGAATTCATAAACTTTTTA
  AGTGTGGGATTTCCTTCGTAAGCAAAAAAAAAAGTTGATTTATTTAAACCTTGGGATGGAGATCAAAACG
  GAATTTCATTTGCCGGGATTTCCTCCCCCTTTGAGGACCCTCTTGTGGAAAAATTTAAAGGAGGGGGGGG
  GGGGAAAAAGAAAATTGCACCCGGGGGGGGAGAAACATTTGAAATTTTTTTTAAAAAGCGGGGCAAAAAG
  GGGGGCAAAAAAAAAAAACCCCTTTAAAAATTTCTCCCCCAGAAAAAAAGAGGGGCCCCCCCCCCTTTTT
  TTCACGGGTTCAAAGGGGAAAATCCCCCCCCTCCCCCCGAGGCGGCGGGGTCTTTTTTTTTAAAAAAAAA
  TAGGGCGGCGGGTGGCGGGGAAACCTGGGGGGAACCACCCCCTGAGCGTGGGGGGGATTTACTCTCCCGA
  TGTTATAGAGCATGGGGGAGTGTTTTCTTTTCCCAGGAACTCTTTCTCACATGGTTGGTAGTAATCTCAC
  GCGTGTATCAGAAGAACATATCACCCACCCCTATTTGCGTTGTGCGGAGTAGGGGAGGATATAGTCTACG
  CTAATATAACTACGCGATCATTCATGTCTCCGTTTCATATTAGCGATATACTCTTATCTGAGAGCCGCTC
  ACAGCATTCATA
  
  Kinase domain sequence

  >SC323613 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TCTTCGTAATACGGTCTGGCTTCTTTTCTGAGTGTTCAAGGTACGACACCGAGCTTCTGGTCAGGTGATG
  GCTCTTATGATGAACACATTGAGCAGTAACCGGGCAAACATGCTGAAAGAAGTACAGCTCATGAATAGAC
  TCTCCCATCCCAACATCCTTAGGTTCATGGGTGTATGTGTTCATCAAGGACAATTGCATGCACTTACAGA
  GTATATCAACTCCGGGAACCTGGAACAGTTGCTAGACAGTAACCT
  
• Restriction Sites: Please inquire
• ACCN: NM_007170
• ORF Size: 1668 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_007170.1, NP_009101.1
• RefSeq Size: 3016
• RefSeq ORF: 1668
• Locus ID: 10420
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene product is a serine/threonine protein kinase that contains an N-terminal protein kinase domain that is structurally similar to the kinase domains of testis-specific protein kinase-1 and the LIM motif-containing protein kinases (LIMKs). Its overall structure is most related to the former, indicating that it belongs to the TESK subgroup of the LIMK/TESK family of protein kinases. This gene is predominantly expressed in testis and prostate. The developmental expression pattern of the rat gene in testis suggests an important role for this gene in meitoic stages and/or early stages of spermiogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
  Transcript Variant: This variant (1) encodes the longer isoform (1).