LCK (NM_005356) Human Untagged Clone

CAT#: SC323616

LCK (untagged)-Kinase deficient mutant (K273M) of Human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LCK
• Synonyms: IMD22; LSK; p56lck; pp58lck; YT16
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005356, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCTGTGGCTGCAGCTCACACCCGGAAGATGACTGGATGGAAAACATCGATGTGTGTGAGAACTGCC
  ATTATCCCATAGTCCCACTGGATGGCAAGGGCACGCTGCTCATCCGAAATGGCTCTGAGGTGCGGGACCC
  ACTGGTTACCTACGAAGGCTCCAATCCGCCGGCTTCCCCACTGCAAGACAACCTGGTTATCGCTCTGCAC
  AGCTATGAGCCCTCTCACGACGGAGATCTGGGCTTTGAGAAGGGGGAACAGCTCCGCATCCTGGAGCAGA
  GCGGCGAGTGGTGGAAGGCGCAGTCCCTGACCACGGGCCAGGAAGGCTTCATCCCCTTCAATTTTGTGGC
  CAAAGCGAACAGCCTGGAGCCCGAACCCTGGTTCTTCAAGAACCTGAGCCGCAAGGACGCGGAGCGGCAG
  CTCCTGGCGCCCGGGAACACTCACGGCTCCTTCCTCATCCGGGAGAGCGAGAGCACCGCGGGATCGTTTT
  CACTGTCGGTCCGGGACTTCGACCAGAACCAGGGAGAGGTGGTGAAACATTACAAGATCCGTAATCTGGA
  CAACGGTGGCTTCTACATCTCCCCTCGAATCACTTTTCCCGGCCTGCATGAACTGGTCCGCCATTACACC
  AATGCTTCAGATGGGCTGTGCACACGGTTGAGCCGCCCCTGCCAGACCCAGAAGCCCCAGAAGCCGTGGT
  GGGAGGACGAGTGGGAGGTTCCCAGGGAGACGCTGAAGCTGGTGGAGCGGCTGGGGGCTGGACAGTTCGG
  GGAGGTGTGGATGGGGTACTACAACGGGCACACGAAGGTGGCGGTGAAGAGCCTGAAGCAGGGCAGCATG
  TCCCCGGACGCCTTCCTGGCCGAGGCCAACCTCATGAAGCAGCTGCAACACCAGCGGCTGGTTCGGCTCT
  ACGCTGTGGTCACCCAGGAGCCCATCTACATCATCACTGAATACATGGAGAATGGGAGTCTAGTGGATTT
  TCTCAAGACCCCTTCAGGCATCAAGTTGACCATCAACAAACTCCTGGACATGGCAGCCCAAATTGCAGAA
  GGCATGGCATTCATTGAAGAGCGGAATTATATTCATCGTGACCTTCGGGCTGCCAACATTCTGGTGTCTG
  ACACCCTGAGCTGCAAGATTGCAGACTTTGGCCTAGCACGCCTCATTGAGGACAACGAGTACACAGCCAG
  GGAGGGGGCCAAGTTTCCCATTAAGTGGACAGCGCCAGAAGCCATTAACTACGGGACATTCACCATCAAG
  TCAGATGTGTGGTCTTTTGGGATCCTGCTGACGGAAATTGTCACCCACGGCCGCATCCCTTACCCAGGGA
  TGACCAACCCGGAGGTGATTCAGAACCTGGAGCGAGGCTACCGCATGGTGCGCCCTGACAACTGTCCAGA
  GGAGCTGTACCAACTCATGAGGCTGTGCTGGAAGGAGCGCCCAGAGGACCGGCCCACCTTTGACTACCTG
  CGCAGTGTGCTGGAGGACTTCTTCACGGCCACAGAGGGCCAGTACCAGCCTCAGCCTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005356 unedited
  CCCGCCCGTCCAGCACTGGGCGGTAGGCGCTGTACGGCTGGGAGGTTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGACGGCGAA
  GGGAGCTGAGACTGTCCAGGCAGCCAGGCTAGGCCAGGAGGACCATGTGAATGGGGCCAGAGGGCTCCCG
  GGCTGGGCAGGGACCATGGGCTGTGGCTGCAGCTCACACCCGGAAGATGACTGGATGGAAAACATCGATG
  TGTGTGAGAACTGCCATTATCCCATAGTCCCACTGGATGGCAAGGCACGCTGCTCATCCGAAATGGCTCT
  GAGGTGCGGGGACCCACTTGGTTACCTACCGGAAGGCCTCCCATTTCCGCCCGGCTTTCCCCACTGTGCA
  AGGGTGACCCCCAGGCCAGCAGGGGCCTGGAAAAACCAAGGCCCTGCCGGATCCCTGGCCTGGTTGCCCT
  CCCCCCCTCTTCCCCCACCCTACTTTTTTCCCCGTTCCTGGCTTTTCTTGTGCCCCCCCCCCCTGGTACC
  TCCAAGGCTTCTGCCCCGATCCCAGTCTGGTTTCCCCCGGTACCCCCTTTTGTTTTTCCAAAAAACCTGG
  TTTTATCTCTGCACAGCGTTGAAACCCCCTCCCCACAAGAAAATTGGGGGTTTAGAAAAGGGGGAAAAGC
  CCGCATTCCCCGGGACGCGAATTTTTTTTTTAAATTTTGTTTGGGGAAACCCCGGGGGGGGAGCGGATTT
  TCCCGACCCCAACCCCCCTGGGGGGGCCTTTATCAACCTTTGGGGGGGCCCCCCTTTTGACAAAAATTTT
  AGGCTCCCTTATTTTTCTGAACCCCGGGTTGGGGGGAGCTGGGTTATAGGGGGGTAGGAGGTTTTTTAGA
  AGGAGGTCTATACTACTCACTTATCACCCACTCTCCCCACCCTCGTGGTGTCAGAAGGCGATTGGTGGAG
  AGCCCTATTTCCCGAGCCCGAAGGTATGCCTTTTATGTTGTGCACAGAGACAATCCGTGTACAGCGACGC
  TCTTTTATACAAACGTATCAT
  
  Kinase domain sequence

  >SC323616 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GATGCWGCGCCTGRTACTGKGRCGGCTGGGGCTGGMAGTTCGGGGAGGTGTGGATGGGGTACTACAACGG
  GCACACGAAGGTGGCGGTGATGAGCCTGAAGCAGGGCAGCATGTCCCCGGACGCCTTCCTGGCCGAGGCC
  AACCTCATGAAGCAGCTGCAACACCAGCGGCTGGTTCGGCTCTACGCTGKGGTCACCCAGGAGCCCATCT
  ACATCATCACTGAATACATGGARAATGGGAGTCTAGTGGATTTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_005356
• Insert Size: 2740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005356.2, NP_005347.2
• RefSeq Size: 2032 bp
• RefSeq ORF: 1530 bp
• Locus ID: 3932
• Cytogenetics: 1p35.2
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway
• Gene Summary: 'This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]'
  Transcript Variant: This variant (2) is transcribed from the distal type II promoter and contains a distinct 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a).