JNK1 (MAPK8) (NM_139049) Human Untagged Clone

CAT#: SC323625

MAPK8 (untagged)-Kinase deficient mutant (K55M) of Human mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPK8
• Synonyms: JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139049, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCAGAAGCAAGCGTGACAACAATTTTTATAGTGTAGAGATTGGAGATTCTACATTCACAGTCCTGA
  AACGATATCAGAATTTAAAACCTATAGGCTCAGGAGCTCAAGGAATAGTATGCGCAGCTTATGATGCCAT
  TCTTGAAAGAAATGTTGCAATCAAGAAGCTAAGCCGACCATTTCAGAATCAGACTCATGCCAAGCGGGCC
  TACAGAGAGCTAGTTCTTATGAAATGTGTTAATCACAAAAATATAATTGGCCTTTTGAATGTTTTCACAC
  CACAGAAATCCCTAGAAGAATTTCAAGATGTTTACATAGTCATGGAGCTCATGGATGCAAATCTTTGCCA
  AGTGATTCAGATGGAGCTAGATCATGAAAGAATGTCCTACCTTCTCTATCAGATGCTGTGTGGAATCAAG
  CACCTTCATTCTGCTGGAATTATTCATCGGGACTTAAAGCCCAGTAATATAGTAGTAAAATCTGATTGCA
  CTTTGAAGATTCTTGACTTCGGTCTGGCCAGGACTGCAGGAACGAGTTTTATGATGACGCCTTATGTAGT
  GACTCGCTACTACAGAGCACCCGAGGTCATCCTTGGCATGGGCTACAAGGAAAACGTGGATTTATGGTCT
  GTGGGGTGCATTATGGGAGAAATGGTTTGCCACAAAATCCTCTTTCCAGGAAGGGACTATATTGATCAGT
  GGAATAAAGTTATTGAACAGCTTGGAACACCATGTCCTGAATTCATGAAGAAACTGCAACCAACAGTAAG
  GACTTACGTTGAAAACAGACCTAAATATGCTGGATATAGCTTTGAGAAACTCTTCCCTGATGTCCTTTTC
  CCAGCTGACTCAGAACACAACAAACTTAAAGCCAGTCAGGCAAGGGATTTGTTATCCAAAATGCTGGTAA
  TAGATGCATCTAAAAGGATCTCTGTAGATGAAGCTCTCCAACACCCGTACATCAATGTCTGGTATGATCC
  TTCTGAAGCAGAAGCTCCACCACCAAAGATCCCTGACAAGCAGTTAGATGAAAGGGAACACACAATAGAA
  GAGTGGAAAGAATTGATATATAAGGAAGTTATGGACTTGGAGGAGAGAACCAAGAATGGAGTTATACGGG
  GGCAGCCCTCTCCTTTAGGTGCAGCAGTGATCAATGGCTCTCAGCATCCATCATCATCGTCGTCTGTCAA
  TGATGTGTCTTCAATGTCAACAGATCCGACTTTGGCCTCTGATACAGACAGCAGTCTAGAAGCAGCAGCT
  GGGCCTCTGGGCTGCTGTAGATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_139049 unedited
  ACCGCACGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACGACGCGGCTT
  GGATTGCGGAGCCGCGAGCAGCGCTGGGTAACGGCCGCGGCGACCACCCCGGACGGCCCCTGTCCCCGCT
  GGCGGGCTTCCCTGTCGCCGTTCGCTGCGCTGCCGGCTTCTTGGTGAATTTTTGGATGAAGCCATTAAAT
  TAATTGCTTGCCATCATGAGCAGAAGCAAGCGTGACAACAATTTTTTATAGTGTAGAGATTGGAGATTTT
  TCTTTTCACAGTCCTGAACCAATATCAGAAATTTAAAACCTATAGGCTCAGGAGCTTCAAGGGAAAAGTA
  GGCCCCACCTTAAGAATGCCCTTCCTTGAAAGAAATGTTGCAATCCTGAAACCTTAACCCAACCCATTTT
  CAAATCCAAATTCTGGCCCACCGGGCCCTTACAGAAGAGATGGTTTTTTTTTGAAGGGTGTTAATTCCCC
  AAAATTTTATTTGGCCCTTTTGAAAGGGTTTTCCCCCCCCCAAAAATCCCTAAAAAAATTTTCGAAATTT
  TTCCATACTCGGGGGCCCTGGGGGGGCCAATTTTTTCCCAGGGTTTTAAGAGGGCCCCTTCTTTAGAAAA
  AAATGCTCCACCTTTTTTTTCCAGATGGGGGGGGGGAACCCCCCCCTTTTTTGTGGGGGGTTTTTTTTCG
  GGGTCTATACCCCCCATATTTTTGTAAAAACTTGTCGCCCTTTAAAAATTCCTATTGCGGGCGCGCCGCT
  GCGCGCACCTTTTTTTTAACGCCTCTTTTGAGTCGCCTCCCTACACCCCCGCGATATTCTCTGGGTGTAC
  AAGAACTAAATTTATATTGTGGGGGGGGTCATAAGAAAATAATCCCAAGCCTCCTCTTAGGCGAGGACAT
  TTCTCATAAATTAATAGCATATATACGCATACCCCACAAGAACGTAGCCGT
  
  Kinase domain sequence

  >SC323625 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CKCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACGACGCGGCTTGGATTG
  CGGAGCCGCGAGCAGCGCTGGGTAACGGCCGCGGCGACCACCCCGGACGGCCCCTGTCCCCGCTGGCGGG
  CTTCCCTGTCGCCGTTCGCTGCGCTGCCGGCTTCTTGGTGAATTT
  
• Restriction Sites: Please inquire
• ACCN: NM_139049
• Insert Size: 6100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139049.1, NP_620637.1
• RefSeq Size: 1412 bp
• RefSeq ORF: 1284 bp
• Locus ID: 5599
• Cytogenetics: 10q11.22
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]'
  Transcript Variant: This variant (JNK1-a2) encodes the longer of the two JNK1 alpha isoforms (JNK1 alpha2). The JNK1-a2 variant differs from the JNK1-b2 variant in the use of an alternate internal coding exon of the same length. Thus, JNK1 alpha2 isoform is the same length as JNK1 beta2 isoform, with a few aa difference in an internal protein segment. Variants JNK1-a2, 14, 15 and 17 all encode isoform alpha2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.