ICK (NM_016513) Human Untagged Clone

CAT#: SC323626

ICK (untagged)-Kinase deficient mutant (K33M) of Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ICK
• Synonyms: ECO; EJM10; LCK2; MRK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323626 sequence for NM_016513 edited (data generated by NextGen Sequencing)
  ATGAATAGATACACAACAATCAGGCAGCTCGGGGATGGAACCTACGGTTCCGTCCTGCTG
  GGAAGAAGCATTGAGTCTGGGGAGCTGATCGCTATTAAAAAAATGAAAAGAAAATTTTAT
  TCCTGGGAGGAATGCATGAACCTTCGGGAGGTTAAGTCTTTAAAGAAGCTCAACCATGCC
  AATGTAGTCAAATTAAAAGAAGTTATCAGGGAAAATGATCATCTTTATTTTATCTTCGAG
  TACATGAAGGAAAATCTTTACCAGCTCATTAAAGAGAGAAATAAGTTGTTTCCTGAGTCT
  GCTATAAGGAATATCATGTATCAGATATTACAAGGACTCGCATTTATTCACAAACACGGC
  TTCTTTCATCGAGACTTAAAGCCTGAGAACCTCCTCTGCATGGGACCAGAACTTGTGAAA
  ATTGCAGACTTTGGTTTGGCCCGAGAAATACGATCAAAACCTCCATATACAGATTATGTA
  TCTACCAGATGGTACAGGGCTCCAGAAGTACTCCTGAGGTCTACCAACTACAGCTCCCCC
  ATTGACGTCTGGGCGGTGGGCTGCATCATGGCAGAAGTTTACACCCTCAGGCCACTCTTC
  CCTGGAGCCAGTGAAATTGACACAATATTCAAAATTTGCCAAGTGCTGGGGACACCAAAA
  AAGACTGACTGGCCTGAAGGCTATCAACTTTCAAGTGCAATGAACTTCCGTTGGCCACAG
  TGTGTACCCAATAACTTAAAGACCTTGATTCCCAATGCTAGCAGTGAAGCAGTCCAGCTC
  CTGAGAGACATGCTTCAGTGGGATCCCAAGAAACGACCAACAGCTAGTCAGGCACTTCGA
  TATCCTTACTTCCAAGTTGGACACCCACTAGGCAGCACCACACAAAACCTTCAGGATTCA
  GAAAAACCACAGAAAGGCATCCTGGAAAAGGCAGGCCCACCTCCTTATATTAAGCCAGTC
  CCACCTGCCCAGCCACCAGCCAAGCCACACACACGAATTTCTTCACGACAGCATCAAGCC
  AGCCAGCCCCCTCTGCATCTCACGTACCCCTACAAAGCAGAGGTCTCCAGGACAGATCAC
  CCAAGCCATCTCCAGGAGGACAAGCCAAGCCCGTTGCTTTTCCCATCCCTCCACAACAAG
  CATCCACAGTCGAAAATCACAGCTGGCCTGGAGCACAAAAATGGTGAGATAAAGCCAAAG
  AGTAGGAGAAGGTGGGGTCTTATTTCCAGGTCAACAAAGGATTCAGATGATTGGGCTGAC
  TTGGATGACTTGGATTTCAGTCCATCCCTCAGCAGGATTGACCTGAAAAACAAGAAAAGA
  CAGAGTGATGACACTCTCTGCAGGTTTGAGAGTGTTTTGGACCTGAAGCCCTCTGAGCCT
  GTGGGCACAGGAAACAGTGCCCCCACCCAGACGTCATATCAGCGGCGAGACACGCCCACC
  CTGAGATCTGCAGCCAAGCAGCACTATTTGAAGCACTCTCGATACTTGCCTGGGATCAGT
  ATAAGAAATGGCATACTCTCGAATCCAGGCAAGGAATTTATTCCACCTAATCCATGGTCT
  AGTTCTGGCTTGTCTGGAAAATCTTCAGGGACAATGTCAGTAATCAGCAAAGTAAATTCA
  GTTGGTTCCAGCTCTACAAGTTCTAGTGGACTGACTGGAAACTATGTCCCTTCCTTTCTG
  AAAAAAGAAATCGGTTCTGCTATGCAGAGGGTACACCTAGCACCTATTCCAGACCCTTCC
  CCTGGTTATTCCTCCCTGAAGGCCATGAGACCTCATCCTGGGCGACCATTCTTCCACACC
  CAGCCTAGAAGCACTCCTGGGTTGATACCACGGCCTCCAGCCGCCCAGCCAGTGCATGGC
  CGGACAGACTGGGCTTCCAAGTACGCATCTCGGCGATGA
  
  Clone variation with respect to NM_016513.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_016513 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGTGGAAAACGGCCGGG
  TTCCTGCTGCGCTACCATGCGCCGTGCGGCCCGTGCGACTCGCCGGACCTCGCGGGCGTCCCTGTACGGA
  GCCCTCGGCCGGTCCTAGCAGGGATTGTCCCCATTTCCAGCTCCGGAGCGGGCGGCTGCGCCCCGCTCGT
  CGAGGAGCTGCGCTCACCTCAGGGGCGGGCCCCCCGCCTGCGTTCGCGGCGCCAGCAGAAGACTGGTTGT
  TTGGAAATATGTATTTGGGAGACCAGTCACGTCCTTATTGAAATACCTTGTGCTGGGTGCTTGCCATCGA
  AAAATTCTGGTACCACTTCTGGGGGGAGGACTGCTACCACTGCAGAACTGGACCACTTCGGCCTTGAGAT
  GAGGGTCCGGCCTGGAGCAAGGACACCATGAATTGGTTACACACATCAGCAGCCTGGGGAAGGGAACTCA
  CGGTTCCGTTCTCGCGGGAAAAACCTTGAATCCGGGGACGCTTATCGATATAAGAAAAGGAAGAAAATTT
  TTTCGGGAGAGATGCTGACCTTCGGAAGTTAAGCTTTAAGAACTCAACAGCCATGTGTTCATTAGGAGTT
  TCCGGAAGTATCTTTTTTATTCCGTACTGAGGATCTTCCGCCTATAAGGAATACTGTTTCGTCGCTAAGA
  ATCGTTCAATTCCAGCGCTATTCAACCGGG
  
  Kinase domain sequence

  >SC323626 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CKAMGCACATGATAGATACACAACAATCAGGCAGCTCGGGGATGGAACCTACGGTTCCGTCCTGCTGGGA
  AGAAGCATTGAGTCTGGGGAGCTGATCGCTATTATGAAAATGAAAAGAAAATTTTATTCCTGGGAGGAAT
  GCATGAACCTTCGGGAGGTTAAGTCTTTAAAGAAGCTCAACCATGCCAATGTAGTCAAATTAAAAGAAGT
  TATCAGGGAAAATGATCATCTTTATTTTATCTTCGAGTACATGAA
  
• Restriction Sites: Please inquire
• ACCN: NM_016513
• ORF Size: 1899 bp
• Insert Size: 3800
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_016513.3, NP_057597.2
• RefSeq Size: 6228
• RefSeq ORF: 1899
• Locus ID: 22858
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) has an additional exon in the 5' UTR, as compared to variant 1. Variants 1 and 2 encode the same protein.