DYRK1B (NM_004714) Human Untagged Clone

CAT#: SC323632

DYRK1B (untagged)-Kinase deficient mutant (K140M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DYRK1B
• Synonyms: AOMS3; MIRK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323632 sequence for NM_004714 edited (data generated by NextGen Sequencing)
  ATGGCCGTCCCACCGGGCCATGGTCCCTTCTCTGGCTTCCCAGGGCCCCAGGAGCACACG
  CAGGTATTGCCTGATGTGCGGCTACTGCCTCGGAGGCTGCCCCTGGCCTTCCGGGATGCA
  ACCTCAGCCCCGCTGCGTAAGCTCTCTGTGGACCTCATCAAGACCTACAAGCACATCAAT
  GAGGTATACTATGCGAAGAAGAAGCGGCGGGCCCAGCAGGCGCCACCCCAGGATTCGAGC
  AACAAGAAGGAGAAGAAGGTCCTGAACCATGGTTATGATGACGACAACCATGACTACATC
  GTGCGCAGTGGCGAGCGCTGGCTGGAGCGCTACGAAATTGACTCGCTCATTGGCAAAGGC
  TCCTTTGGCCAGGTGGTGAAAGCCTATGATCATCAGACCCAGGAGCTTGTGGCCATCATG
  ATCATCAAGAACAAAAAGGCTTTCCTGAACCAGGCCCAGATTGAGCTGCGGCTGCTGGAG
  CTGATGAACCAGCATGACACGGAGATGAAGTACTATATAGTACACCTGAAGCGGCACTTC
  ATGTTCCGGAACCACCTGTGCCTGGTATTTGAGCTGCTGTCCTACAACCTGTACGACCTC
  CTGCGCAACACCCACTTCCGCGGCGTCTCGCTGAACCTGACCCGGAAGCTGGCGCAGCAG
  CTCTGCACGGCACTGCTCTTTCTGGCCACGCCTGAGCTCAGCATCATTCACTGCGACCTC
  AAGCCCGAAAACATCTTGCTGTGCAACCCCAAGCGCAGCGCCATCAAGATTGTGGACTTC
  GGCAGCTCCTGCCAGCTTGGCCAGAGGATCTACCAGTATATCCAGAGCCGCTTCTACCGC
  TCACCTGAGGTGCTCCTGGGCACACCCTACGACCTGGCCATTGACATGTGGTCCCTGGGC
  TGCATCCTTGTGGAGATGCACACCGGAGAGCCCCTCTTCAGTGGCTCCAATGAGGTCGAC
  CAGATGAACCGCATTGTGGAGGTGCTGGGCATCCCACCGGCCGCCATGCTGGACCAGGCG
  CCCAAGGCTCGCAAGTACTTTGAACGGCTGCCTGGGGGTGGCTGGACCCTACGAAGGACG
  AAAGAACTCAGGAAGGATTACCAGGGCCCCGGGACACGGCGGCTGCAGGAGGTGCTGGGC
  GTGCAGACGGGCGGGCCCGGGGGCCGGCGGGCGGGGGAGCCGGGCCACAGCCCCGCCGAC
  TACCTCCGCTTCCAGGACCTGGTGCTGCGCATGCTGGAGTATGAGCCCGCCGCCCGCATC
  AGCCCCCTGGGGGCTCTGCAGCACGGCTTCTTCCGCCGCACGGCCGACGAGGCCACCAAC
  ACGGGCCCGGCAGGCAGCAGTGCCTCCACCTCGCCCGCGCCCCTCGACACCTGCCCCTCT
  TCCAGCACCGCCAGCTCCATCTCCAGTTCTGGAGGCTCCAGTGGCTCCTCCAGTGACAAC
  CGGACCTACCGCTACAGCAACCGATATTGTGGGGGCCCTGGGCCCCCTATCACAGACTGT
  GAGATGAACAGCCCCCAGGTCCCACCCTCCCAGCCGCTGCGGCCCTGGGCAGGGGGTGAT
  GTGCCCCACAAGACACATCAAGCCCCTGCCTCTGCCTCGTCACTGCCTGGGACCGGGGCC
  CAGTTACCCCCCCAGCCCCGATACCTTGGTCGTCCCCCATCACCAACCTCACCACCACCC
  CCGGAGCTGATGGATGTGAGCCTGGTGGGCGGCCCTGCTGACTGCTCCCCACCTCACCCA
  GCGCCTGCCCCCCAGCACCCGGCTGCCTCAGCCCTCCGGACTCGGATGACTGGAGGTCGT
  CCACCCCTCCCGCCTCCTGATGACCCTGCCACTCTGGGGCCTCACCTGGGCCTCCGTGGT
  GTACCCCAGAGCACAGCAGCCAGCTCGTGA
  
  Clone variation with respect to NM_004714.1
  419 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004714 unedited
  GCGCCCGTTTGAGCAATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAGGGCGTGGG
  GGGGCCGGTTTGTTGTGGTCGCCATTTTGCTGGTTGCATTACTGGGTAATCGGGGCCCTGGCTTGCCGCG
  TCCGCCGGATACCCTCAGCCAGTGGGCAGGTCTGAGCTCGGGCTCCCCGAGCAGTTTGAGTCCCCTTGCC
  CGCTCCTTCAGGTCTCAGCGGCGGTGGCAGCCGAGGTGCAGGGATGCAAGAAGCGCCCCCCCGGCCGGGC
  TCCCGCTCCAGGCCTCGCTCCCCTGCGGCCCTCTGAGCCCAACCCATTGCCCGGTCCCCACCCGGGCAAT
  TGTTCCCTTTCTCTGCCTCCCCAGGGCCCAGGAACCACCCCCCGGGATTTTGCCTGATGTGCGGCTACGG
  CCTCGAAGGCTGCCCCTGCCCTTCGGGTTCCAACCTCGCCCCCGTTCCGTAGCCTTCTGGGGGACCTTAT
  CGACCTTACGCCCACATCTGAGAGTGTACTCTGCGCAAAAAAAACCCGGGGCCCCGCGGGCCCCACCCCG
  ATTTTCGCCCCACGGGAGGGAGAGAGGGCCGGAATGGGTTTTATGAGGACAACACGAGATATCTTGCGCA
  CAGTGCGCACCGCTTCTGAACTCTAAAAATTGCTCTCTTGTGGAAAGGCTTTTTGCGCAGGTGGAAGACT
  CTATATTCTACCACGAGCTGTGGCCTCTGATGTTATAACACAGCGTCTCATAACGCCCATAATCGCCGCT
  GCTGGCGCCTGACACTGCACGCAGTGAAGACTTCTGTAGCTTAACAGCGCTATGCTGGACACCTGCCGCG
  ATTGATAGCGTCGCACATGTGAGACTCGGCGAACCACTTC
  
  Kinase domain sequence

  >SC323632 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AGGCGRCGCTGGCTGGRCGCTACGAAATTGACTCGCTCATTGGCAAAGGCTCCTTTGGCCAGGTGGTGAA
  AGCCTATGATCATCAGACCCAGGAGCTTGTGGCCATCATGATCATCAAGAACAAAAAGGCTTTCCTGAAC
  CAGGCCCAGATTGAGCTGCGGCTGCTGGAGCTGATGAACCAGCATGACACGGAGATGAAGTACTATATAG
  TACACCTGAAGCGGCACTTCATGTTCCGGAACCACCTGTGCCTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_004714
• ORF Size: 1890 bp
• Insert Size: 2487
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_004714.1, NP_004705.1
• RefSeq Size: 2540
• RefSeq ORF: 1890
• Locus ID: 9149
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Gene Summary: This gene encodes a member of a family of nuclear-localized protein kinases. The encoded protein participates in the regulation of the cell cycle. Expression of this gene may be altered in tumor cells, and mutations in this gene were found to cause abdominal obesity-metabolic syndrome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
  Transcript Variant: This variant (1, also known as 'a') represents the longest transcript and encodes the longest isoform (p69, also known as isoform a).