BMPR1A (NM_004329) Human Untagged Clone

CAT#: SC323635

BMPR1A (untagged)-Kinase deficient mutant (K261M) of Human bone morphogenetic protein receptor, type IA (BMPR1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BMPR1A
• Synonyms: 10q23del; ACVRLK3; ALK3; CD292; SKR5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004329, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTCAGCTATACATTTACATCAGATTATTGGGAGCCTATTTGTTCATCATTTCTCGTGTTCAAGGAC
  AGAATCTGGATAGTATGCTTCATGGCACTGGGATGAAATCAGACTCCGACCAGAAAAAGTCAGAAAATGG
  AGTAACCTTAGCACCAGAGGATACCTTGCCTTTTTTAAAGTGCTATTGCTCAGGGCACTGTCCAGATGAT
  GCTATTAATAACACATGCATAACTAATGGACATTGCTTTGCCATCATAGAAGAAGATGACCAGGGAGAAA
  CCACATTAGCTTCAGGGTGTATGAAATATGAAGGATCTGATTTTCAGTGCAAAGATTCTCCAAAAGCCCA
  GCTACGCCGGACAATAGAATGTTGTCGGACCAATTTATGTAACCAGTATTTGCAACCCACACTGCCCCCT
  GTTGTCATAGGTCCGTTTTTTGATGGCAGCATTCGATGGCTGGTTTTGCTCATTTCTATGGCTGTCTGCA
  TAATTGCTATGATCATCTTCTCCAGCTGCTTTTGTTACAAACATTATTGCAAGAGCATCTCAAGCAGACG
  TCGTTACAATCGTGATTTGGAACAGGATGAAGCATTTATTCCAGTTGGAGAATCACTAAAAGACCTTATT
  GACCAGTCACAAAGTTCTGGTAGTGGGTCTGGACTACCTTTATTGGTTCAGCGAACTATTGCCAAACAGA
  TTCAGATGGTCCGGCAAGTTGGTAAAGGCCGATATGGAGAAGTATGGATGGGCAAATGGCGTGGCGAAAA
  AGTGGCGGTGAAAGTATTCTTTACCACTGAAGAAGCCAGCTGGTTTCGAGAAACAGAAATCTACCAAACT
  GTGCTAATGCGCCATGAAAACATACTTGGTTTCATAGCGGCAGACATTAAAGGTACAGGTTCCTGGACTC
  AGCTCTATTTGATTACTGATTACCATGAAAATGGATCTCTCTATGACTTCCTGAAATGTGCTACACTGGA
  CACCAGAGCCCTGCTTAAATTGGCTTATTCAGCTGCCTGTGGTCTGTGCCACCTGCACACAGAAATTTAT
  GGCACCCAAGGAAAGCCCGCAATTGCTCATCGAGACCTAAAGAGCAAAAACATCCTCATCAAGAAAAATG
  GGAGTTGCTGCATTGCTGACCTGGGCCTTGCTGTTAAATTCAACAGTGACACAAATGAAGTTGATGTGCC
  CTTGAATACCAGGGTGGGCACCAAACGCTACATGGCTCCCGAAGTGCTGGACGAAAGCCTGAACAAAAAC
  CACTTCCAGCCCTACATCATGGCTGACATCTACAGCTTCGGCCTAATCATTTGGGAGATGGCTCGTCGTT
  GTATCACAGGAGGGATCGTGGAAGAATACCAATTGCCATATTACAACATGGTACCGAGTGATCCGTCATA
  CGAAGATATGCGTGAGGTTGTGTGTGTCAAACGTTTGCGGCCAATTGTGTCTAATCGGTGGAACAGTGAT
  GAATGTCTACGAGCAGTTTTGAAGCTAATGTCAGAATGCTGGGCCCACAATCCAGCCTCCAGACTCACAG
  CATTGAGAATTAAGAAGACGCTTGCCAAGATGGTTGAATCCCAAGATGTAAAAATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004329 unedited
  TCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGAAGGCTTAGGCTCG
  GCGCGTCCGTCCGCGCGCGGCGAAGATCGCACGGCCCGATCGAGGGGCGACCGGGTCGGGGCCGCTGCAC
  GCCAAGGGCGAAGGCCGATCCGGGCCCCACTTCGCCCCGGCGGCTCGCCGCGCCCACCCGCTCCGCGCCG
  AGGGCTGGAGGATGCGTTCCCTGGGGTCCGGACTTATGAAAATATGCATCAGTTTAATACTGTCTGTGGA
  ATTCATGAGATGGAAGCATAGGTCAAAGCTGTTTGGAGAAAATCAGAGGTACAGTTTTTATCTAGCCACA
  TCTTTGGAGGGAGTCGTAAGAAAAGCAGGTGGAAGTTGAGGTCATTGTCAGTTGCTGGCGATCTTTTACA
  AGAAAATCCTCACTGGATGGATAGTCATTTTAAATGGTGGAGGTAGCAGAAACAATATTAAAGGGTGACG
  TACAAGGAAACATACCAATTGACCATGGACTACGCTATCCTTTTCATACGATTATGGGAACCCAAATGGT
  CTCACATTCTCTCGTGTACAGGACAGACTCGGGAGTGTGGGCCACGGGCACTGGAGAGAATTCAGATCGC
  ACCGGAAGTTCAAATGGGTATACCTTACCCAGGAACCTGGCTTTTTAATGGCATGGCAGGCCTGCAATGG
  CTATACACAGTCACATGGCATGCGTTGCTCTAAGAGTGCCGGAACCATCTCGGTGAGTAAGATGATATGC
  GAGTACGCGCGAATCCGATTAGTTGACAGATGAAGCATGAGCCAGGCCAGGTAAAGGCCG
  
  Kinase domain sequence

  >SC323635 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GWTGGMTMGCGACTATTGCYAACAGATTCAGATGGTCCGGCAAKTTGGTAAAGGCCGATATGGAGAAGTA
  TGGATGGGSAAATGGCGTGGCGAAAAAGTGGCGGTGATGGTATTCTTTACCACTGAAGAAGCCAGCTGGT
  TTCGAGAAACAGAAATCTACCAAACTGTGCTAATGCGCCATGAAAACATACTTGGTTTCATAGCGGCAGA
  CATTAAAGGTACAGGTTCCTGGACTCAGCTCTATTTGATTACTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_004329
• Insert Size: 3370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004329.2, NP_004320.2
• RefSeq Size: 3631 bp
• RefSeq ORF: 1599 bp
• Locus ID: 657
• Cytogenetics: 10q23.2
• Domains: Activin_recp, pkinase, TyrKc, S_TKc, GS
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, TGF-beta signaling pathway
• Gene Summary: 'The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. [provided by RefSeq, Jul 2008]'