TXK (NM_003328) Human Untagged Clone

CAT#: SC323664

TXK (untagged)-Kinase deficient mutant (K299M) of Human TXK tyrosine kinase (TXK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TXK
• Synonyms: BTKL; PSCTK5; PTK4; RLK; TKL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003328, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCCTTTCCTCCTATAACACCATCCAGTCGGTTTTCTGTTGCTGCTGTTGCTGTTCAGTGCAGAAGC
  GACAAATGAGAACACAGATAAGCCTGAGCACAGATGAAGAGCTTCCAGAAAAATACACCCAGCGTCGCAG
  GCCGTGGCTCAGCCAATTGTCAAATAAGAAGCAATCCAACACGGGCCGTGTGCAGCCGTCAAAACGAAAG
  CCACTGCCTCCCCTCCCACCCTCTGAGGTTGCTGAAGAGAAGATCCAAGTCAAGGCACTTTATGATTTTC
  TGCCCAGAGAACCCTGTAATTTAGCCTTAAGGAGAGCAGAAGAATACCTGATACTGGAGAAATACAATCC
  TCACTGGTGGAAGGCAAGAGACCGTTTGGGGAATGAAGGCTTAATCCCAAGCAACTATGTGACTGAAAAC
  AAAATAACTAATTTAGAAATATATGAGTGGTACCATAGAAACATTACCAGAAATCAGGCAGAACATCTAT
  TGAGACAAGAGTCTAAAGAAGGTGCATTTATTGTCAGAGATTCAAGACATTTAGGATCCTACACAATTTC
  CGTATTTATGGGAGCTAGAAGAAGTACGGAGGCTGCCATAAAACATTATCAGATAAAAAAGAATGACTCA
  GGACAGTGGTATGTGGCTGAAAGACACGCCTTTCAATCAATCCCTGAGTTAATCTGGTATCACCAGCACA
  ATGCAGCCGGTCTCATGACTCGTCTCCGATATCCAGTTGGGCTGATGGGCAGTTGTTTACCAGCCACAGC
  TGGGTTTAGCTACGAAAAGTGGGAGATAGATCCATCTGAGTTGGCTTTTATAAAGGAGATTGGAAGCGGT
  CAGTTTGGAGTGGTCCATTTAGGTGAATGGCGGTCACATATCCAGGTAGCTATCAAGGCCATCAATGAAG
  GCTCCATGTCTGAAGAGGATTTCATTGAAGAGGCCAAAGTGATGATGAAATTATCTCATTCAAAGCTAGT
  GCAACTTTATGGAGTCTGTATACAGCGGAAGCCCCTTTACATTGTGACAGAGTTCATGGAAAATGGCTGC
  CTGCTTAACTATCTCAGGGAGAATAAAGGAAAGCTTAGGAAGGAAATGCTACTGAGTGTATGCCAGGATA
  TATGTGAAGGAATGGAATATCTGGAGAGGAATGGCTATATTCATAGGGATTTGGCGGCAAGGAATTGTTT
  GGTCAGTTCAACATGCATAGTAAAAATTTCAGACTTTGGAATGACAAGGTACGTTTTGGATGATGAGTAT
  GTCAGTTCTTTTGGAGCCAAGTTCCCAATCAAGTGGTCCCCTCCTGAAGTTTTTCTTTTCAATAAGTACA
  GCAGTAAATCTGATGTCTGGTCATTTGGAGTTTTAATGTGGGAAGTTTTTACAGAAGGAAAAATGCCTTT
  TGAAAATAAGTCAAATTTGCAAGTCGTGGAAGCTATTTCTGAAGGCTTCAGGCTATATCGCCCTCACCTG
  GCACCAATGTCCATATATGAAGTCATGTACAGCTGCTGGCATGAGAAACCTGAAGGCCGCCCTACATTTG
  CCGAGCTGCTGCGGGCTGTCACAGAGATTGCGGAAACCTGGTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_003328
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003328.1, NP_003319.1
• RefSeq Size: 2564 bp
• RefSeq ORF: 1584 bp
• Locus ID: 7294
• Cytogenetics: 4p12
• Domains: pkinase, SH2, SH3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: ''