LIM kinase 2 (LIMK2) (NM_016733) Human Untagged Clone

CAT#: SC323676

LIMK2 (untagged)-Kinase deficient mutant (K360M) of Human LIM domain kinase 2 (LIMK2), transcript variant 2b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIMK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323676 sequence for NM_016733 edited (data generated by NextGen Sequencing)
  ATGGGGAGTTACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCA
  GAATGCCAGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCC
  AAGGACTACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGG
  CCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGCTGC
  AAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTACTGT
  GGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAGTCT
  GTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGC
  AGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAA
  GTGAAAGAGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGG
  GACCGCATCCTGGAGATCAATGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTGGAG
  GATGCAATTAGCCAGACGAGCCAGACACTTCAGCTGTTGATTGAACATGACCCCGTCTCC
  CAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACATGCAGAATGCCGGA
  CACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGGAGA
  CGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAG
  CCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTAT
  TCACAGCAGATCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTC
  TTTGGGCAGGCTATCAAGGTGACACACAAAGCCACGGGCAAAGTGATGGTCATGAAAGAG
  TTAATTCGATGTGATGAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATGCGC
  AGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAAGGATAAGAAGCTG
  AACCTCCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATGGAT
  CCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTAT
  TTGCACTCTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTG
  GACAAGACTGTGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAA
  AGGGCCCCCATGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGACCGCAAG
  AAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGGCCCCTGAGATGCTGAACGGAAAG
  AGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTGTGAGATCATTGGG
  CAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTGAAG
  CTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCC
  ATCTGCTGCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCCTTT
  GAGGCCCTCTCCCTGTACCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAGGAG
  TTGGACCACACTGTGAGCATGCAGTACGGCCTGACCCGGGACTCACCTCCCTAG
  
  Clone variation with respect to NM_016733.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_016733 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGCGCGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAAATGAGAACATA
  CAGCCGAGAATACTGCCGAAGCTGAGACTGACTACTGTGCATTAGGAAAGACCTGGAGTCAGGACTTTGG
  TGGGATTTGGAGCTCCGAGGCAGTAATAACTGAACAAGCAGCCCTGTCCCCTAGGCTGCAGAAGCTTGAA
  TGCATCCTCTCCCAGAACCTGCCACAGGAAACTGGGGGCTTTGTCAGGTGAAGATGTCTGGAGGGTGTCC
  AGGCTGTGGGGACCACATTTGCTCCAAGCCAGATATGGGTACAGGACTGGTCAACGAAACCTGGGCACGG
  CTCTTGCTTCGGGGTTCAGAATGCCAGGATTCCCTCACAACTGGGTACTATGAGAAGGATGGGAAGCTTT
  ACTGCCCAGGACTACTGGGGGAAGTTTGGGGAGTCGGCCTGGGTGCTCCTCTGATGACGGGCCTTTTTGG
  TGGCCTGGGGGAGTCAGTTACCCCCCAAGGCTTTCCTGTTGACCTGCAGGAATATGGGAGAGGGAATGCT
  TTGATGGGTGCACATGCCACTCTCTCTGTGGAAGTCCAAGAGTGTGGCGTGCCACTGTTTGAGAAACTCC
  CAGTTTGTTCGAGACCTCCCTATTGTACCTAATCTAGCGCGCCACTTAGACGACGCGGCTTCTGGGTCGG
  GGAAGCGTTGCCATCACCCCACATTGACCTGAAGTTAGCAGTAACAATTCCACTGAGCTCCTGGACCATC
  GTATGACCCCTGGCCACTCTGAGAAGAGACTCT
  
  Kinase domain sequence

  >SC323676 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGAWCTTCGACTGTGACTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTG
  ACACACAAAGCCACGGGCAAAGTGATGGTCATGATGGAGTTAATTCGATGTGATGAGGAGACCCAGAAAA
  CTTTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCT
  GTACAAGGATAAGAAGCTGAACCTGCTGACAGAGTACATTGAGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_016733
• Insert Size: 4130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016733.2, NP_057952.1
• RefSeq Size: 3848 bp
• RefSeq ORF: 1854 bp
• Locus ID: 3985
• Cytogenetics: 22q12.2
• Domains: pkinase, TyrKc, PDZ, LIM, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: 'There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2b) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (2b) is shorter and has a distinct C-terminus compared to isoform 1.