LIM kinase 2 (LIMK2) (NM_016733) Human Untagged Clone

CAT#: SC323676

LIMK2 (untagged)-Kinase deficient mutant (K360M) of Human LIM domain kinase 2 (LIMK2), transcript variant 2b


  "NM_016733" in other vectors (5)

Reconstitution Protocol

USD 1,050.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol LIMK2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323676 sequence for NM_016733 edited (data generated by NextGen Sequencing)
ATGGGGAGTTACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCA
GAATGCCAGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCC
AAGGACTACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGG
CCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGCTGC
AAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTACTGT
GGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAGTCT
GTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGC
AGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAA
GTGAAAGAGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGG
GACCGCATCCTGGAGATCAATGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTGGAG
GATGCAATTAGCCAGACGAGCCAGACACTTCAGCTGTTGATTGAACATGACCCCGTCTCC
CAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACATGCAGAATGCCGGA
CACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGGAGA
CGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAG
CCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTAT
TCACAGCAGATCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTC
TTTGGGCAGGCTATCAAGGTGACACACAAAGCCACGGGCAAAGTGATGGTCATGAAAGAG
TTAATTCGATGTGATGAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATGCGC
AGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAAGGATAAGAAGCTG
AACCTCCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATGGAT
CCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTAT
TTGCACTCTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTG
GACAAGACTGTGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAA
AGGGCCCCCATGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGACCGCAAG
AAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGGCCCCTGAGATGCTGAACGGAAAG
AGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTGTGAGATCATTGGG
CAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTGAAG
CTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCC
ATCTGCTGCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCCTTT
GAGGCCCTCTCCCTGTACCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAGGAG
TTGGACCACACTGTGAGCATGCAGTACGGCCTGACCCGGGACTCACCTCCCTAG

Clone variation with respect to NM_016733.2
>OriGene 5' read for mutant NM_016733 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGCGCGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAAATGAGAACATA
CAGCCGAGAATACTGCCGAAGCTGAGACTGACTACTGTGCATTAGGAAAGACCTGGAGTCAGGACTTTGG
TGGGATTTGGAGCTCCGAGGCAGTAATAACTGAACAAGCAGCCCTGTCCCCTAGGCTGCAGAAGCTTGAA
TGCATCCTCTCCCAGAACCTGCCACAGGAAACTGGGGGCTTTGTCAGGTGAAGATGTCTGGAGGGTGTCC
AGGCTGTGGGGACCACATTTGCTCCAAGCCAGATATGGGTACAGGACTGGTCAACGAAACCTGGGCACGG
CTCTTGCTTCGGGGTTCAGAATGCCAGGATTCCCTCACAACTGGGTACTATGAGAAGGATGGGAAGCTTT
ACTGCCCAGGACTACTGGGGGAAGTTTGGGGAGTCGGCCTGGGTGCTCCTCTGATGACGGGCCTTTTTGG
TGGCCTGGGGGAGTCAGTTACCCCCCAAGGCTTTCCTGTTGACCTGCAGGAATATGGGAGAGGGAATGCT
TTGATGGGTGCACATGCCACTCTCTCTGTGGAAGTCCAAGAGTGTGGCGTGCCACTGTTTGAGAAACTCC
CAGTTTGTTCGAGACCTCCCTATTGTACCTAATCTAGCGCGCCACTTAGACGACGCGGCTTCTGGGTCGG
GGAAGCGTTGCCATCACCCCACATTGACCTGAAGTTAGCAGTAACAATTCCACTGAGCTCCTGGACCATC
GTATGACCCCTGGCCACTCTGAGAAGAGACTCT
>SC323676 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TGAWCTTCGACTGTGACTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTG
ACACACAAAGCCACGGGCAAAGTGATGGTCATGATGGAGTTAATTCGATGTGATGAGGAGACCCAGAAAA
CTTTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCT
GTACAAGGATAAGAAGCTGAACCTGCTGACAGAGTACATTGAGGG
Restriction Sites Please inquire     
ACCN NM_016733
Insert Size 4130 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016733.2, NP_057952.1
RefSeq Size 3848 bp
RefSeq ORF 1854 bp
Locus ID 3985
Cytogenetics 22q12.2
Domains pkinase, TyrKc, PDZ, LIM, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
Gene Summary 'There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2b) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (2b) is shorter and has a distinct C-terminus compared to isoform 1.

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